LOCUS       Exported                6199 bp DNA     circular SYN 10-OCT-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pBBR1MCS2-Tac-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1792..1810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1840..1856
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1910..1987)
                     /label=lacI promoter
     promoter        2033..2061
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             2108..2815
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      2852..2899
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2941..2957)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2990..3008)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3018..3034)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3746..4405)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(4406..5177)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
     1861 gcttgatatc gaattcgagc tcggtacctc gcgaatgcat ctagatcaca ttcaccaccc
     1921 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     1981 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa atttgacaat
     2041 taatcatcgg ctcgtataat gtgtggaatg tctcatgagc ggatatttca cacaggaaac
     2101 agtattcatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     2161 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     2221 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     2281 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     2341 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     2401 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     2461 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     2521 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     2581 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     2641 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     2701 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     2761 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaatt
     2821 aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     2881 ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacacatcg
     2941 gatccactag ttctagagcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     3001 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3061 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3121 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg
     3181 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3241 accaataggc cgtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
     3301 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
     3361 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     3421 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     3481 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     3541 tgatcattta ttctgcctcc cagagcctga taaaaacggt gaatccgtta gcgaggtgcc
     3601 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
     3661 gcagacaagg tatagggcgg cgaggcggct acagccgata gtctggaaca gcgcacttac
     3721 gggttgctgc gcaacccaag tgctaccggc gcggcagcgt gacccgtgtc ggcggctcca
     3781 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc
     3841 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc
     3901 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca
     3961 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat
     4021 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg
     4081 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg
     4141 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc
     4201 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg
     4261 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg
     4321 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc
     4381 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtaggggtgc cgcacggttg
     4441 cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt
     4501 aaaagtggca gtcaattaca gattttcttt aacctacgca atgagctatt gcggggggtg
     4561 ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt gatttttaag tctttcgcat
     4621 ttcgccctat atctagttct ttggtgccca aagaagggca cccctgcggg gttcccccac
     4681 gccttcggcg cggctccccc tccggcaaaa agtggcccct ccggggcttg ttgatcgact
     4741 gcgcggcctt cggccttgcc caaggtggcg ctgccccctt ggaacccccg cactcgccgc
     4801 cgtgaggctc ggggggcagg cgggcgggct tcgcccttcg actgccccca ctcgcatagg
     4861 cttgggtcgt tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga
     4921 cccgccttcc acttggtgtc caaccggcaa gcgaagcgcg caggccgcag gccggaggct
     4981 tttccccaga gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg cagttggagc
     5041 cggtgggtat gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt caagctcgtg
     5101 ggcaggcgca gcctgtccat cagcttgtcc agcagggttg tccacgggcc gagcgaagcg
     5161 agccagccgg tggccgctcg cggccatcgt ccacatatcc acgggctggc aagggagcgc
     5221 agcgaccgcg cagggcgaag cccggagagc aagcccgtag ggcgccgcag ccgccgtagg
     5281 cggtcacgac tttgcgaagc aaagtctagt gagtatactc aagcattgag tggcccgccg
     5341 gaggcaccgc cttgcgctgc ccccgtcgag ccggttggac accaaaaggg aggggcaggc
     5401 atggcggcat acgcgatcat gcgatgcaag aagctggcga aaatgggcaa cgtggcggcc
     5461 agtctcaagc acgcctaccg cgagcgcgag acgcccaacg ctgacgccag caggacgcca
     5521 gagaacgagc actgggcggc cagcagcacc gatgaagcga tgggccgact gcgcgagttg
     5581 ctgccagaga agcggcgcaa ggacgctgtg ttggcggtcg agtacgtcat gacggccagc
     5641 ccggaatggt ggaagtcggc cagccaagaa cagcaggcgg cgttcttcga gaaggcgcac
     5701 aagtggctgg cggacaagta cggggcggat cgcatcgtga cggccagcat ccaccgtgac
     5761 gaaaccagcc cgcacatgac cgcgttcgtg gtgccgctga cgcaggacgg caggctgtcg
     5821 gccaaggagt tcatcggcaa caaagcgcag atgacccgcg accagaccac gtttgcggcc
     5881 gctgtggccg atctagggct gcaacggggc atcgagggca gcaaggcacg tcacacgcgc
     5941 attcaggcgt tctacgaggc cctggagcgg ccaccagtgg gccacgtcac catcagcccg
     6001 caagcggtcg agccacgcgc ctatgcaccg cagggattgg ccgaaaagct gggaatctca
     6061 aagcgcgttg agacgccgga agccgtggcc gaccggctga caaaagcggt tcggcagggg
     6121 tatgagcctg ccctacaggc cgccgcagga gcgcgtgaga tgcgcaagaa ggccgatcaa
     6181 gcccaagaga cggcccgag
//
