LOCUS       62056_3695       10947 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        1792..1810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1877..3316
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             3332..4252
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4304..5383
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5401..6381
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             6563..7672
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     promoter        complement(7742..7760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(8496..9155)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(9156..9925)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
     1861 gcttgatatc gaattcatga ctaaaaaaat ttcattcatt attaacggcc aggttgaaat
     1921 ctttcccgaa agtgatgatt tagtgcaatc cattaatttt ggtgataata gtgtttacct
     1981 gccaatattg aatgactctc atgtaaaaaa cattattgat tgtaatggaa ataacgaatt
     2041 acggttgcat aacattgtca attttctcta tacggtaggg caaagatgga aaaatgaaga
     2101 atactcaaga cgcaggacat acattcgtga cttaaaaaaa tatatgggat attcagaaga
     2161 aatggctaag ctagaggcca attggatatc tatgatttta tgttctaaag gcggccttta
     2221 tgatgttgta gaaaatgaac ttggttctcg ccatatcatg gatgaatggc tacctcagga
     2281 tgaaagttat gttcgggctt ttccgaaagg taaatctgta catctgttgg caggtaatgt
     2341 tccattatct gggatcatgt ctatattacg cgcaatttta actaagaatc agtgtattat
     2401 aaaaacatcg tcaaccgatc cttttaccgc taatgcatta gcgttaagtt ttattgatgt
     2461 agaccctaat catccgataa cgcgctcttt atctgttata tattggcccc accaaggtga
     2521 tacatcactc gcaaaagaaa ttatgcgaca tgcggatgtt attgtcgctt ggggagggcc
     2581 agatgcgatt aattgggcgg tagagcatgc gccatcttat gctgatgtga ttaaatttgg
     2641 ttctaaaaag agtctttgca ttatcgataa tcctgttgat ttgacgtccg cagcgacagg
     2701 tgcggctcat gatgtttgtt tttacgatca gcgagcttgt ttttctgccc aaaacatata
     2761 ttacatggga aatcattatg aggaatttaa gttagcgttg atagaaaaac ttaatctata
     2821 tgcgcatata ttaccgaatg ccaaaaaaga ttttgatgaa aaggcggcct attctttagt
     2881 tcaaaaagaa agcttgtttg ctggattaaa agtagaggtg gatattcatc aacgttggat
     2941 gattattgag tcaaatgcag gtgtggaatt taatcaacca cttggcagat gtgtgtacct
     3001 tcatcacgtc gataatattg agcaaatatt gccttatgtt caaaaaaata agacgcaaac
     3061 catatctatt tttccttggg agtcatcatt taaatatcga gatgcgttag cattaaaagg
     3121 tgcggaaagg attgtagaag caggaatgaa taacatattt cgagttggtg gatctcatga
     3181 cggaatgaga ccgttgcaac gattagtgac atatatttct catgaaaggc catctaacta
     3241 tacggctaag gatgttgcgg ttgaaataga acagactcga ttcctggaag aagataagtt
     3301 ccttgtattt gtcccataat aggtaaaaag tatggaaaat gaatcaaaat ataaaaccat
     3361 cgaccacgtt atttgtgttg aaggaaataa aaaaattcat gtttgggaaa cgctgccaga
     3421 agaaaacagc ccaaagagaa agaatgccat tattattgcg tctggttttg cccgcaggat
     3481 ggatcatttt gctggtctgg cggaatattt atcgcggaat ggatttcatg tgatccgcta
     3541 tgattcgctt caccacgttg gattgagttc agggacaatt gatgaattta caatgtctat
     3601 aggaaagcag agcttgttag cagtggttga ttggttaact acacgaaaaa taaataactt
     3661 cggtatgttg gcttcaagct tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa
     3721 tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta agatattctc ttgaaagagc
     3781 tttagggttt gattatctca gtctacccat taatgaattg ccggataatc tagattttga
     3841 aggccataaa ttgggtgctg aagtctttgc gagagattgt cttgattttg gttgggaaga
     3901 tttagcttct acaattaata acatgatgta tcttgatata ccgtttattg cttttactgc
     3961 aaataacgat aattgggtca agcaagatga agttatcaca ttgttatcaa atattcgtag
     4021 taatcgatgc aagatatatt ctttgttagg aagttcgcat gacttgagtg aaaatttagt
     4081 ggtcctgcgc aatttttatc aatcggttac gaaagccgct atcgcgatgg ataatgatca
     4141 tctggatatt gatgttgata ttactgaacc gtcatttgaa catttaacta ttgcgacagt
     4201 caatgaacgc cgaatgagaa ttgagattga aaatcaagca atttctctgt cttaaaatct
     4261 attgagatat tctatcactc aaatagcaat ataaggactc tctatgaaat ttggaaactt
     4321 tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa
     4381 attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt
     4441 cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac
     4501 taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca
     4561 acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg
     4621 ccgagggctt tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc
     4681 cttagcggaa tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc
     4741 tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg
     4801 tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt
     4861 tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga
     4921 gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt
     4981 atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt
     5041 tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca
     5101 aacaagaggt tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa
     5161 agatactaat cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga
     5221 atgtattgac ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg
     5281 atttgaagct aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga
     5341 tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta tattagctaa ggagaaagaa
     5401 atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca agaacaaagt
     5461 atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga acagatttta
     5521 gtgtatgaaa atcatttttc agataatggt gttgtcggcg ctcctctgac tgtttctggt
     5581 tttctgctcg gtttaacaga gaaaattaaa attggttcat taaatcacat cattacaact
     5641 catcatcctg tcgccatagc ggaggaagct tgcttattgg atcagttaag tgaagggaga
     5701 tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg
     5761 gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga tgctttaaca
     5821 acaggctatt gtaatccaga taacgatttt tatagcttcc ctaaaatatc tgtaaatccc
     5881 catgcttata cgccaggcgg acctcggaaa tatgtaacag caaccagtca tcatattgtt
     5941 gagtgggcgg ccaaaaaagg tattcctctc atctttaagt gggatgattc taatgatgtt
     6001 agatatgaat atgctgaaag atataaagcc gttgcggata aatatgacgt tgacctatca
     6061 gagatagacc atcagttaat gatattagtt aactataacg aagatagtaa taaagctaaa
     6121 caagagacgc gtgcatttat tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc
     6181 gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg gaaattatac ggagtgtata
     6241 actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa
     6301 ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata ttgttgatga taatattaag
     6361 aagtaccaca tggaatatac ctaatagatt tcgagttgca gcgaggcggc aagtgaacga
     6421 atccccagga gcatagataa ctatgtgact ggggtgagtg aaagcagcca acaaagcagc
     6481 agcttgaaag atgaagggta taaaagagta tgacagcagt gctgccatac tttctaatat
     6541 tatcttgagg agtaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag
     6601 ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca
     6661 ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg
     6721 agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga
     6781 catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa
     6841 cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg
     6901 tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg
     6961 tagttggttt gatcatcaaa tagaattagt caatttggga ccagatagat ttaatgctca
     7021 taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac
     7081 cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa
     7141 tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta
     7201 tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg
     7261 cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt
     7321 tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact
     7381 caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc
     7441 gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag
     7501 ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat
     7561 aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa
     7621 tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttgaattcc
     7681 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccaattcg
     7741 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7801 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7861 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7921 gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     7981 gctcattttt taaccaatag gccgactgcg atgagtggca gggcggggcg taattttttt
     8041 aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg
     8101 gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt
     8161 taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg
     8221 accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata
     8281 attgacgata tgatcattta ttctgcctcc cagagcctga taaaaacggt gaatccgtta
     8341 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     8401 acgcggggag gcagacaagg tatagggcgg cgaggcggct acagccgata gtctggaaca
     8461 gcgcacttac gggttgctgc gcaacccaag tgctaccggc gcggcagcgt gacccgtgtc
     8521 ggcggctcca acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc
     8581 tgctgcccgc gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg
     8641 cccggaatgc cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg
     8701 cccggataca gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg
     8761 tcgtcgtgat caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc
     8821 tggccccacg ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc
     8881 ttcacgacgg agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc
     8941 aaagaacgtc cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg
     9001 cccatctgcg ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc
     9061 ccggcccacg cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg
     9121 gcttgaatgc cgatttctct ggactgcgtg gccatgctta tctccatgcg gtagggtgcc
     9181 gcacggttgc ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga caacaattat
     9241 gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg
     9301 cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt
     9361 ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg
     9421 ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt
     9481 tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc
     9541 actcgccgcc gtgaggctcg gggggcaggc gggcgggctt cgccttcgac tgcccccact
     9601 cgcataggct tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg
     9661 agccttgacc cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc
     9721 cggaggcttt tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca
     9781 gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca
     9841 agctcgtggg caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga
     9901 gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa
     9961 gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc
    10021 gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg
    10081 gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag
    10141 gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg
    10201 tggcggccag tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca
    10261 ggacgccaga gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc
    10321 gcgagttgct gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga
    10381 cggccagccc ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga
    10441 aggcgcacaa gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc
    10501 accgtgacga aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca
    10561 ggctgtcggc caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt
    10621 ttgcggccgc tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc
    10681 acacgcgcat tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca
    10741 tcagcccgca agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg
    10801 gaatctcaaa gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc
    10861 ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg
    10921 ccgatcaagc ccaagagacg gcccgag
//
