LOCUS       Exported                4811 bp DNA     circular SYN 05-AUG-2024
DEFINITION  Exported.
ACCESSION   V009255
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Yero D, Ferrer-Navarro M, Costenaro L, Planell R, Conchillo-Sole O, 
            Sole M, Roca I, Gibert I, Daura X.
  TITLE     A transport system associated with the intrinsic antimicrobial 
            multiresistance in Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Yero D, Gibert I.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Genetica Molecular Bacteriana, Institut de 
            Biotecnologia i de Biomedicina (IBB), Campus UAB, Bellaterra, 
            Barcelona 08191, Spain
REFERENCE   3  (bases 1 to 4811)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4811)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4811)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-DEC-2016) Genetica Molecular Bacteriana, Institut de 
            Biotecnologia i de Biomedicina (IBB), Campus UAB, Bellaterra, 
            Barcelona 08191, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        126..144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    153..260
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     CDS             402..1136
                     /gene="ermBP"
                     /label=rRNA adenine N-6-methyltransferase
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(1544..2548)
                     /codon_start=1
                     /product="mob encodes the plasmid mobilization functions
                     that allow conjugal delivery of the plasmids into a variety
                     of bacteria from E. coli strains harboring the RK2 conjugal
                     transfer functions."
                     /label=mob
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLAAL"
     promoter        complement(2616..2667)
                     /label=promoter for mob
     misc_feature    2627..2649
                     /label=RSA
                     /note="transfer origins (also called recombination site A 
                     [RSA]), is known as the specific site necessary for 
                     mobilization and recombination mediated by a Mob/Pre 
                     protein. "
     rep_origin      2772..3541
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3542..4201
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
       61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
      121 gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
      181 gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc
      241 tcgagggggg gcccggtacc gaaacgtaaa agaagttatg gaaataagac ttagaagcaa
      301 acttaagagt gtgttgatag tgcagtatct taaaattttg tataatagga attgaagtta
      361 aattagatgc taaaaatttg taattaagaa ggagtgatta catgaacaaa aatataaaat
      421 attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa caattgaatt
      481 taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta acgacgaaac
      541 tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta ttcaacttat
      601 cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat attctacagt
      661 ttcaattccc taacaaacag aggtataaaa ttgttgggag tattccttac catttaagca
      721 cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat ctgattgttg
      781 aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg ctcttgcaca
      841 ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat cctaaaccaa
      901 aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca gataaatatt
      961 ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt caactgttta
     1021 ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta agtaccgtta
     1081 cttatgagca agtattgtct atttttaata gttatctatt atttaacggg aggaaataat
     1141 tctatgagtc gcttttttaa atttggaaag ttacacgtta ctaaagggaa tggagataaa
     1201 ttattagata tactactgac agcttccaag aagctaaaga ggtccctagc gcctacgggg
     1261 aatttgtaag atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat
     1321 tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat
     1381 ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat
     1441 acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca
     1501 agccgagatc ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac gccgccaggt
     1561 ggcacttttc ggggaaatgt gcgcgcccgc gttcctgctg gcgctgggcc tgtttctggc
     1621 gctggacttc ccgctgttcc gtcagcagct tttcgcccac ggccttgatg atcgcggcgg
     1681 ccttggcctg catatcccga ttcaacggcc ccagggcgtc cagaacgggc ttcaggcgct
     1741 cccgaaggtc tcgggccgtc tcttgggctt gatcggcctt cttgcgcatc tcacgcgctc
     1801 ctgcggcggc ctgtagggca ggctcatacc cctgccgaac cgcttttgtc agccggtcgg
     1861 ccacggcttc cggcgtctca acgcgctttg agattcccag cttttcggcc aatccctgcg
     1921 gtgcataggc gcgtggctcg accgcttgcg ggctgatggt gacgtggccc actggtggcc
     1981 gctccagggc ctcgtagaac gcctgaatgc gcgtgtgacg tgccttgctg ccctcgatgc
     2041 cccgttgcag ccctagatcg gccacagcgg ccgcaaacgt ggtctggtcg cgggtcatct
     2101 gcgctttgtt gccgatgaac tccttggccg acagcctgcc gtcctgcgtc agcggcacca
     2161 cgaacgcggt catgtgcggg ctggtttcgt cacggtggat gctggccgtc acgatgcgat
     2221 ccgccccgta cttgtccgcc agccacttgt gcgccttctc gaagaacgcc gcctgctgtt
     2281 cttggctggc cgacttccac cattccgggc tggccgtcat gacgtactcg accgccaaca
     2341 cagcgtcctt gcgccgcttc tctggcagca actcgcgcag tcggcccatc gcttcatcgg
     2401 tgctgctggc cgcccagtgc tcgttctctg gcgtcctgct ggcgtcagcg ttgggcgtct
     2461 cgcgctcgcg gtaggcgtgc ttgagactgg ccgccacgtt gcccattttc gccagcttct
     2521 tgcatcgcat gatcgcgtat gccgccatgc ctgcccctcc cttttggtgt ccaaccggct
     2581 cgacgggggc agcgcaaggc ggtgcctccg gcgggccact caatgcttga gtatactcac
     2641 tagactttgc ttcgcaaagt cgtgaccgcc tacggcggct gcggcgccct acgggcttgc
     2701 tctccgggct tcgccctgcg cggtcgctgc gctcccttgc cagcccgtgg atatgtggac
     2761 gatggccgcg agcggccacc ggctggctcg cttcgctcgg cccgtggaca accctgctgg
     2821 acaagctgat ggacaggctg cgcctgccca cgagcttgac cacagggatt gcccaccggc
     2881 tacccagcct tcgaccacat acccaccggc tccaactgcg cggcctgcgg ccttgcccca
     2941 tcaatttttt taattttctc tggggaaaag cctccggcct gcggcctgcg cgcttcgctt
     3001 gccggttgga caccaagtgg aaggcgggtc aaggctcgcg cagcgaccgc gcagcggctt
     3061 ggccttgacg cgcctggaac gacccaagcc tatgcgagtg ggggcagtcg aaggcgaagc
     3121 ccgcccgcct gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc
     3181 caccttgggc aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt
     3241 tgccggaggg ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg
     3301 gcaccaaaga actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa
     3361 aggggggtac gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa
     3421 gaaaatctgt aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa
     3481 agttgcagct gattgcgcat ggtgccgcaa ccgtgcggca ccctaccgca tggagataag
     3541 catggccacg cagtccagag aaatcggcat tcaagccaag aacaagcccg gtcactgggt
     3601 gcaaacggaa cgcaaagcgc atgaggcgtg ggccgggctt attgcgagga aacccacggc
     3661 ggcaatgctg ctgcatcacc tcgtggcgca gatgggccac cagaacgccg tggtggtcag
     3721 ccagaagaca ctttccaagc tcatcggacg ttctttgcgg acggtccaat acgcagtcaa
     3781 ggacttggtg gccgagcgct ggatctccgt cgtgaagctc aacggccccg gcaccgtgtc
     3841 ggcctacgtg gtcaatgacc gcgtggcgtg gggccagccc cgcgaccagt tgcgcctgtc
     3901 ggtgttcagt gccgccgtgg tggttgatca cgacgaccag gacgaatcgc tgttggggca
     3961 tggcgacctg cgccgcatcc cgaccctgta tccgggcgag cagcaactac cgaccggccc
     4021 cggcgaggag ccgcccagcc agcccggcat tccgggcatg gaaccagacc tgccagcctt
     4081 gaccgaaacg gaggaatggg aacggcgcgg gcagcagcgc ctgccgatgc ccgatgagcc
     4141 gtgttttctg gacgatggcg agccgttgga gccgccgaca cgggtcacgc tgccgcgccg
     4201 gtagcacttg ggttgcgcag caacccgtaa gtgcgctgtt ccagactatc ggctgtagcc
     4261 gcctcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     4321 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcaccg tttttatcag
     4381 gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg
     4441 agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     4501 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
     4561 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
     4621 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     4681 actcatcgca gtcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4741 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
     4801 ggaagggcga t
//
