LOCUS       40924_6054        5859 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBBR1MCS-6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Meng J, Wang H, Liu X, Lin J, Pang X, Lin J.
  TITLE     Construction of small plasmid vectors for use in genetic improvement
            of the extremely acidophilic Acidithiobacillus caldus
  JOURNAL   Microbiol. Res. 168 (8), 469-476 (2013)
  PUBMED    23639949
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Meng J, Wang H, Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2013) State Key Laboratory of Microbial 
            Technology, Shandong University, Shanda Nanlu 27#, Jinan, Shandong 
            250100, China
REFERENCE   3  (bases 1 to 5859)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5859)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol. 
            Res."; date: "2013"; volume: "168"; issue: "8"; pages: "469-476"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2013) State Key Laboratory of Microbial Technology, Shandong
            University, Shanda Nanlu 27#, Jinan, Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3215..3322
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3335..3353)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3374..3390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3398..3414)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3422..3452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3467..3488)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             3682..4485
                     /codon_start=1
                     /gene="strA"
                     /product="Sm resistance protein A"
                     /label=strA
                     /protein_id="AGT62528.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3682..4485
                     /gene="strA"
                     /label=strA
     CDS             4485..5321
                     /codon_start=1
                     /gene="strB"
                     /product="Sm resistance protein B"
                     /label=strB
                     /protein_id="AGT62529.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            4485..5321
                     /gene="strB"
                     /label=strB
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
     3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcatt
     3601 catgtttgac agcttatcat ccataaggtt taatgcggta gtttatcaca gttaaattgc
     3661 taacgcagtc aggcaccgtg tatgaatcga actaatattt tttttggtga atcgcattct
     3721 gactggttgc ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg
     3781 catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac
     3841 cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag
     3901 gaacaggagg gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg
     3961 tctggagcgg atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac
     4021 agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc
     4081 gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt
     4141 acgccgctgc acgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa
     4201 gagcgcaccg atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac
     4261 cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc
     4321 tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa
     4381 gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa
     4441 cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg
     4501 tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc
     4561 tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta
     4621 tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg
     4681 gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc
     4741 gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg
     4801 aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga
     4861 tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc
     4921 aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac
     4981 tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc
     5041 tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct
     5101 acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg
     5161 acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt
     5221 atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta
     5281 tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct
     5341 atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg
     5401 attccgcccc ttcccgcaaa cgaccccaaa caagatcttg atcccctgcg ccatcagatc
     5461 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     5521 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
     5581 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
     5641 atagcccagt agctgacatt catcccaggt ggcacttttc ggggaaatgt gcgcgcccgc
     5701 gttcctgctg gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct
     5761 tttcgcccac ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc
     5821 ccagggcgtc cagaacgggc ttcaggcgct cccgaaggt
//
