LOCUS       Exported                4771 bp DNA     circular SYN 31-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4771)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4771)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(229..4771,1..228)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(24..1027)
                     /codon_start=1
                     /product="mob encodes the plasmid mobilization functions
                     that allow conjugal delivery of the plasmids into a variety
                     of bacteria from E. coli strains harboring the RK2 conjugal
                     transfer functions."
                     /label=mob
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLRRC"
     promoter        complement(1095..1146)
                     /label=promoter for mob
     misc_feature    1106..1128
                     /label=RSA
                     /note="transfer origins (also called recombination site A 
                     [RSA]), is known as the specific site necessary for 
                     mobilization and recombination mediated by a Mob/Pre 
                     protein. "
     rep_origin      1251..2022
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2023..2682
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3394..3410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3420..3438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3447..3554
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3567..3585)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3606..3622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3630..3646)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3654..3684)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3699..3720)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3990..4018
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4207..4737
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ccgcggagtt gttcggtaaa ttgtcacaac gccgcaggtg gcacttttcg gggaaatgtg
       61 cgcgcccgcg ttcctgctgg cgctgggcct gtttctggcg ctggacttcc cgctgttccg
      121 tcagcagctt ttcgcccacg gccttgatga tcgcggcggc cttggcctgc atatcccgat
      181 tcaacggccc cagggcgtcc agaacgggct tcaggcgctc ccgaaggtct cgggccgtct
      241 cttgggcttg atcggccttc ttgcgcatct cacgcgctcc tgcggcggcc tgtagggcag
      301 gctcataccc ctgccgaacc gcttttgtca gccggtcggc cacggcttcc ggcgtctcaa
      361 cgcgctttga gattcccagc ttttcggcca atccctgcgg tgcataggcg cgtggctcga
      421 ccgcttgcgg gctgatggtg acgtggccca ctggtggccg ctccagggcc tcgtagaacg
      481 cctgaatgcg cgtgtgacgt gccttgctgc cctcgatgcc ccgttgcagc cctagatcgg
      541 ccacagcggc cgcaaacgtg gtctggtcgc gggtcatctg cgctttgttg ccgatgaact
      601 ccttggccga cagcctgccg tcctgcgtca gcggcaccac gaacgcggtc atgtgcgggc
      661 tggtttcgtc acggtggatg ctggccgtca cgatgcgatc cgccccgtac ttgtccgcca
      721 gccacttgtg cgccttctcg aagaacgccg cctgctgttc ttggctggcc gacttccacc
      781 attccgggct ggccgtcatg acgtactcga ccgccaacac agcgtccttg cgccgcttct
      841 ctggcagcaa ctcgcgcagt cggcccatcg cttcatcggt gctgctggcc gcccagtgct
      901 cgttctctgg cgtcctgctg gcgtcagcgt tgggcgtctc gcgctcgcgg taggcgtgct
      961 tgagactggc cgccacgttg cccattttcg ccagcttctt gcatcgcatg atcgcgtatg
     1021 ccgccatgcc tgcccctccc ttttggtgtc caaccggctc gacgggggca gcgcaaggcg
     1081 gtgcctccgg cgggccactc aatgcttgag tatactcact agactttgct tcgcaaagtc
     1141 gtgaccgcct acggcggctg cggcgcccta cgggcttgct ctccgggctt cgccctgcgc
     1201 ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg atggccgcga gcggccaccg
     1261 gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg gacaggctgc
     1321 gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt cgaccacata
     1381 cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt aattttctct
     1441 ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac accaagtgga
     1501 aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc gcctggaacg
     1561 acccaagcct atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct gccccccgag
     1621 cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag
     1681 gccgcgcagt cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc
     1741 cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata
     1801 gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc
     1861 attgcggcac cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc
     1921 cacttttacg caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat
     1981 ggtgccgcaa ccgtgcggca cccctaccgc atggagataa gcatggccac gcagtccaga
     2041 gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga acgcaaagcg
     2101 catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct gctgcatcac
     2161 ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac actttccaag
     2221 ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt ggccgagcgc
     2281 tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt ggtcaatgac
     2341 cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag tgccgccgtg
     2401 gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct gcgccgcatc
     2461 ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga gccgcccagc
     2521 cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac ggaggaatgg
     2581 gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct ggacgatggc
     2641 gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt gggttgcgca
     2701 gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg ccctatacct
     2761 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
     2821 agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga ggcagaataa
     2881 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
     2941 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
     3001 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
     3061 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag
     3121 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtacggcc
     3181 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3241 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     3301 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     3361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     3421 aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaacta
     3481 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
     3541 gggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     3601 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3661 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3721 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3781 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgcatgca taaaaactgt
     3841 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     3901 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggacgcacac
     3961 cgtggaaacg gatgaaggca cgaacccagt tgacataagc ctgttcggtt cgtaaactgt
     4021 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     4081 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
     4141 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     4201 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     4261 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
     4321 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc cacctactcc
     4381 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
     4441 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
     4501 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg agagcaccgg
     4561 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
     4621 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
     4681 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
     4741 caattcgttc aagccgagat cggcttcccg g
//
