LOCUS       Exported                4950 bp DNA     circular SYN 05-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4950)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4950)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(287..4950,1..286)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(24..1085)
                     /codon_start=1
                     /product="mob encodes the plasmid mobilization 
                     functionsthat allow conjugal delivery of the plasmids into 
                     a variety of bacteria from E. coli strains harboring the 
                     RK2 conjugal transfer functions."
                     /label=mob
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLDFGHEIIKK
                     DLHLDPFKLKMKF"
     promoter        complement(1153..1204)
                     /label=promoter for mob
     misc_feature    1164..1186
                     /label=RSA
                     /note="transfer origins (also called recombination site A 
                     [RSA]), is known as the specific site necessary for 
                     mobilization and recombination mediated by a Mob/Pre 
                     protein. "
     rep_origin      1309..2078
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2079..2738
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3448..3464
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3474..3492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3501..3608
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3621..3639)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3660..3676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3684..3700)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3708..3738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3753..3774)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4002..4073
                     /label=AmpR promoter
     CDS             4074..4931
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
       61 agatcctttt tgataatctc atgaccaaaa tccaggtggc acttttcggg gaaatgtgcg
      121 cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct ggacttcccg ctgttccgtc
      181 agcagctttt cgcccacggc cttgatgatc gcggcggcct tggcctgcat atcccgattc
      241 aacggcccca gggcgtccag aacgggcttc aggcgctccc gaaggtctcg ggccgtctct
      301 tgggcttgat cggccttctt gcgcatctca cgcgctcctg cggcggcctg tagggcaggc
      361 tcatacccct gccgaaccgc ttttgtcagc cggtcggcca cggcttccgg cgtctcaacg
      421 cgctttgaga ttcccagctt ttcggccaat ccctgcggtg cataggcgcg tggctcgacc
      481 gcttgcgggc tgatggtgac gtggcccact ggtggccgct ccagggcctc gtagaacgcc
      541 tgaatgcgcg tgtgacgtgc cttgctgccc tcgatgcccc gttgcagccc tagatcggcc
      601 acagcggccg caaacgtggt ctggtcgcgg gtcatctgcg ctttgttgcc gatgaactcc
      661 ttggccgaca gcctgccgtc ctgcgtcagc ggcaccacga acgcggtcat gtgcgggctg
      721 gtttcgtcac ggtggatgct ggccgtcacg atgcgatccg ccccgtactt gtccgccagc
      781 cacttgtgcg ccttctcgaa gaacgccgcc tgctgttctt ggctggccga cttccaccat
      841 tccgggctgg ccgtcatgac gtactcgacc gccaacacag cgtccttgcg ccgcttctct
      901 ggcagcaact cgcgcagtcg gcccatcgct tcatcggtgc tgctggccgc ccagtgctcg
      961 ttctctggcg tcctgctggc gtcagcgttg ggcgtctcgc gctcgcggta ggcgtgcttg
     1021 agactggccg ccacgttgcc cattttcgcc agcttcttgc atcgcatgat cgcgtatgcc
     1081 gccatgcctg cccctccctt ttggtgtcca accggctcga cgggggcagc gcaaggcggt
     1141 gcctccggcg ggccactcaa tgcttgagta tactcactag actttgcttc gcaaagtcgt
     1201 gaccgcctac ggcggctgcg gcgccctacg ggcttgctct ccgggcttcg ccctgcgcgg
     1261 tcgctgcgct cccttgccag cccgtggata tgtggacgat ggccgcgagc ggccaccggc
     1321 tggctcgctt cgctcggccc gtggacaacc ctgctggaca agctgatgga caggctgcgc
     1381 ctgcccacga gcttgaccac agggattgcc caccggctac ccagccttcg accacatacc
     1441 caccggctcc aactgcgcgg cctgcggcct tgccccatca atttttttaa ttttctctgg
     1501 ggaaaagcct ccggcctgcg gcctgcgcgc ttcgcttgcc ggttggacac caagtggaag
     1561 gcgggtcaag gctcgcgcag cgaccgcgca gcggcttggc cttgacgcgc ctggaacgac
     1621 ccaagcctat gcgagtgggg gcagtcgaag gcgaagcccg cccgcctgcc ccccgagcct
     1681 cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag gccgaaggcc
     1741 gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga gccgcgccga
     1801 aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact agatataggg
     1861 cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca acagctcatt
     1921 gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat tgactgccac
     1981 ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat tgcgcatggt
     2041 gccgcaaccg tgcggcaccc taccgcatgg agataagcat ggccacgcag tccagagaaa
     2101 tcggcattca agccaagaac aagcccggtc actgggtgca aacggaacgc aaagcgcatg
     2161 aggcgtgggc cgggcttatt gcgaggaaac ccacggcggc aatgctgctg catcacctcg
     2221 tggcgcagat gggccaccag aacgccgtgg tggtcagcca gaagacactt tccaagctca
     2281 tcggacgttc tttgcggacg gtccaatacg cagtcaagga cttggtggcc gagcgctgga
     2341 tctccgtcgt gaagctcaac ggccccggca ccgtgtcggc ctacgtggtc aatgaccgcg
     2401 tggcgtgggg ccagccccgc gaccagttgc gcctgtcggt gttcagtgcc gccgtggtgg
     2461 ttgatcacga cgaccaggac gaatcgctgt tggggcatgg cgacctgcgc cgcatcccga
     2521 ccctgtatcc gggcgagcag caactaccga ccggccccgg cgaggagccg cccagccagc
     2581 ccggcattcc gggcatggaa ccagacctgc cagccttgac cgaaacggag gaatgggaac
     2641 ggcgcgggca gcagcgcctg ccgatgcccg atgagccgtg ttttctggac gatggcgagc
     2701 cgttggagcc gccgacacgg gtcacgctgc cgcgccggta gcacttgggt tgcgcagcaa
     2761 cccgtaagtg cgctgttcca gactatcggc tgtagccgcc tcgccgccct ataccttgtc
     2821 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     2881 ggcggcacct cgctaacgga ttcaccgttt ttatcaggct ctgggaggca gaataaatga
     2941 tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg
     3001 agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat
     3061 aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt
     3121 tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac
     3181 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagtc ggcctattgg
     3241 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     3301 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     3361 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     3421 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     3481 actcactata gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat
     3541 cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     3601 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     3661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3841 ggccaacgcg cggggagagg cggtttgcgt attgggcgca tgcataaaaa ctgttgtaat
     3901 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     3961 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccattcaaat atgtatccgc
     4021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4921 ttaagcattg gtaactgtca gaccaagttt
//
