LOCUS       62056_3690        5635 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      2530..2624
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3184..3843)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(3844..4613)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa aaagaggaga
     1741 aatttcacac aggaaacagc tggatccatg cagaacctga acggtaaagt tgctttcgtt
     1801 accggtggtt ctcgtggtat cggtgctgct atcgttcgtc gtctggctgc tgacggtgct
     1861 gacatcgctt tcacctacgt ttctgcttct tctaaaaacg ttgctaccgc tctggttcag
     1921 gaactggaag ctaaaggtcg tcgtgctcgt gctatccagg ctgactctgc tgacccggct
     1981 caggttcgtc aggctgttga acaggctatc gttcagctgg gtccggttga cgttctggtt
     2041 aacaacgctg gtatcttcct ggctggtccg ctgggtgaag ttaccctgga cgactacgaa
     2101 cgtaccatga acatcaacgt tcgtgctccg ttcgttgcta tccaggctgc tcaggcttct
     2161 atgccggacg gtggtcgtat catcaacatc ggttcttgcc tggctgaacg tgctggtcgt
     2221 gctggtgtta ccctgtacgc tgcttctaaa tctgctctgc tgggtatgac ccgtggtctg
     2281 gctcgtgacc tgggtgctcg tggtatcacc gctaacgttg ttcacccggg tccgatcgac
     2341 accgacatga acccggctga cggtgaacgt tctggtgaac tggttgctgt tctgtctctg
     2401 ccgcactacg gtgaagttcg tgacatcgct ggtatggttg ctttcctggc tggtccggac
     2461 ggtcgttacg ttaccggtgc ttctctggct gttgacggtg gtttcgctgc ttagctcgag
     2521 taattttttg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     2581 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatcgttaa tattttgtta
     2641 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgactgcgat
     2701 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
     2761 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
     2821 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
     2881 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
     2941 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
     3001 gagcctgata aaaacggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     3061 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     3121 aggcggctac agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg
     3181 ctaccggcgc ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa
     3241 acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc
     3301 ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc
     3361 gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc
     3421 gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa
     3481 caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta
     3541 ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa
     3601 gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt
     3661 ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat
     3721 tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt
     3781 ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc
     3841 catgcttatc tccatgcggt agggtgccgc acggttgcgg caccatgcgc aatcagctgc
     3901 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
     3961 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
     4021 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
     4081 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
     4141 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     4201 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     4261 gcgggcttcg ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
     4321 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
     4381 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
     4441 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
     4501 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
     4561 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
     4621 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
     4681 gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag
     4741 tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc
     4801 gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga
     4861 tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag
     4921 cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc
     4981 agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac
     5041 gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc
     5101 caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg
     5161 gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg
     5221 ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa
     5281 gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa
     5341 cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg
     5401 gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat
     5461 gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc
     5521 gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc
     5581 gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgag
//
