LOCUS       V009258                 6156 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009258
VERSION     V009258
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Repurposing type III polyketide synthase as a malonyl-CoA biosensor
            for metabolic engineering in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   30232266
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Novel Enzyme-coupled Malonyl-CoA Biosensor based on Type III
            Polyketide Synthase and Use of the same (KR 10-2018-0066323)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6156)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   4  (bases 1 to 6156)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6156)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..31
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    39..55
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             76..1548
                     /gene="zwf"
                     /label="Glucose-6-phosphate 1-dehydrogenase"
                     /note="Glucose-6-phosphate 1-dehydrogenase from Escherichia
                     coli (strain K12). Accession#: P0AC53"
     misc_feature    1554..1609
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      1812..1898
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1990..2017
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    complement(2038..2059)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2173..2805)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2806..2908)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(3058..4077)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label="mob"
                     /protein_id="AYD60185.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(3058..4077)
                     /gene="mob"
                     /label="mob"
     rep_origin      4301..5072
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             5073..5732
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 gttgacaatt aatcatccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agaccatggc ggtaacgcaa acagcccagg cctgtgacct ggtcattttc
      121 ggcgcgaaag gcgaccttgc gcgtcgtaaa ttgctgcctt ccctgtatca actggaaaaa
      181 gccggtcagc tcaacccgga cacccggatt atcggcgtag ggcgtgctga ctgggataaa
      241 gcggcgtata ccaaagttgt ccgcgaggcg ctcgaaactt tcatgaaaga aaccattgat
      301 gaaggtttat gggacaccct gagtgcacgt ctggattttt gtaatctcga tgtcaatgac
      361 actgctgcat tcaaccgtct cggcgcgatg ctggatcaaa aaaatcgtat caccattaac
      421 tactttgcca tgccgcccag cacttttggc gcaatttgca aagggcttgg cgaggcaaaa
      481 ctgaatgcta aaccggcacg cgtagtcatg gagaaaccgc tggggacgtc gctggcgacc
      541 tcgcaggaaa tcaatgatca ggttggcgaa tacttcgagg agtgccaggt ttaccgtatc
      601 gaccactatc ttggtaaaga aacggtgctg aacctgttgg cgctgcgttt tgctaactcc
      661 ctgtttgtga ataactggga caatcgcacc attgatcatg ttgagattac cgtggcagaa
      721 gaagtgggga tcgaagggcg ctggggctat tttgataaag ccggtcagat gcgcgatatg
      781 atccaaaacc acctgctgca aattctctgc atgattgcga tgtctccgcc atctgacctg
      841 agcgcagaca gcatccgcga tgaaaaagtg aaagtactga agtccctgcg ccgcatcgac
      901 cgctccaacg tacgcgaaaa aaccgtacgt gggcaatata ctgcgggctt cgcccagggc
      961 aaaaaagtgc cgggatatct ggaagaagag ggcgcgaaca agagcagcaa tacagaaacc
     1021 ttcgtggcga tccgcgtcga cattgataac tggcgctggg ccggtgtgcc attctacctg
     1081 cgtactggta aacgtctgcc gaccaaatgt tctgaagtcg tggtctattt caaaacacct
     1141 gaactgaatc tgtttaaaga gtcgtggcag gatctgccgc agaataaact gactatccgt
     1201 ctgcaacctg atgaaggcgt ggatatccag gtactgaata aagttcctgg ccttgaccac
     1261 aaacataacc tgcaaatcac caagctggat ctgagctatt cagaaacctt taatcagacg
     1321 catctggcgg atgcctatga acgtctgctg ctggaaacca tgcgtggtat tcaggcactg
     1381 tttgttcgtc gcgacgaagt ggaagaagcc tggaaatggg tagactccat tactgaggcg
     1441 tgggcgatgg acaatgatgc gccgaaaccg tatcaggccg gaacctgggg acccgttgcc
     1501 tcggtggcga tgattacccg tgatggtcgt tcctggaatg agtttgagta atggatccga
     1561 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gctgttttgg
     1621 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     1681 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     1741 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     1801 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1861 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gtcgggagcg gatttgaacg
     1921 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     1981 aaattaagca gaaggccatc ctgacggatg gccttttact agtgcctggg gtgcctaatg
     2041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2161 gcgcatgcat aaaaactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2221 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2281 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2341 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     2401 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2461 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2521 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2581 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2641 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2701 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2761 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2821 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2881 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     2941 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     3001 aggtatttat tcgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3061 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcgcccgcg
     3121 ttcctgctgg cgctgggcct gtttctggcg ctggacttcc cgctgttccg tcagcagctt
     3181 ttcgcccacg gccttgatga tcgcggcggc cttggcctgc atatcccgat tcaacggccc
     3241 cagggcgtcc agaacgggct tcaggcgctc ccgaaggtct cgggccgtct cttgggcttg
     3301 atcggccttc ttgcgcatct cacgcgctcc tgcggcggcc tgtagggcag gctcataccc
     3361 ctgccgaacc gcttttgtca gccggtcggc cacggcttcc ggcgtctcaa cgcgctttga
     3421 gattcccagc ttttcggcca atccctgcgg tgcataggcg cgtggctcga ccgcttgcgg
     3481 gctgatggtg acgtggccca ctggtggccg ctccagggcc tcgtagaacg cctgaatgcg
     3541 cgtgtgacgt gccttgctgc cctcgatgcc ccgttgcagc cctagatcgg ccacagcggc
     3601 cgcaaacgtg gtctggtcgc gggtcatctg cgctttgttg ccgatgaact ccttggccga
     3661 cagcctgccg tcctgcgtca gcggcaccac gaacgcggtc atgtgcgggc tggtttcgtc
     3721 acggtggatg ctggccgtca cgatgcgatc cgccccgtac ttgtccgcca gccacttgtg
     3781 cgccttctcg aagaacgccg cctgctgttc ttggctggcc gacttccacc attccgggct
     3841 ggccgtcatg acgtactcga ccgccaacac agcgtccttg cgccgcttct ctggcagcaa
     3901 ctcgcgcagt cggcccatcg cttcatcggt gctgctggcc gcccagtgct cgttctctgg
     3961 cgtcctgctg gcgtcagcgt tgggcgtctc gcgctcgcgg taggcgtgct tgagactggc
     4021 cgccacgttg cccattttcg ccagcttctt gcatcgcatg atcgcgtatg ccgccatgcc
     4081 tgcccctccc ttttggtgtc caaccggctc gacgggggca gcgcaaggcg gtgcctccgg
     4141 cgggccactc aatgcttgag tatactcact agactttgct tcgcaaagtc gtgaccgcct
     4201 acggcggctg cggcgcccta cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg
     4261 ctcccttgcc agcccgtgga tatgtggacg atggccgcga gcggccaccg gctggctcgc
     4321 ttcgctcggc ccgtggacaa ccctgctgga caagctgatg gacaggctgc gcctgcccac
     4381 gagcttgacc acagggattg cccaccggct acccagcctt cgaccacata cccaccggct
     4441 ccaactgcgc ggcctgcggc cttgccccat caattttttt aattttctct ggggaaaagc
     4501 ctccggcctg cggcctgcgc gcttcgcttg ccggttggac accaagtgga aggcgggtca
     4561 aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc gcctggaacg acccaagcct
     4621 atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct gccccccgag cctcacggcg
     4681 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
     4741 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
     4801 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
     4861 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
     4921 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
     4981 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
     5041 ccgtgcggca cccctaccgc atggagataa gcatggccac gcagtccaga gaaatcggca
     5101 ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga acgcaaagcg catgaggcgt
     5161 gggccgggct tattgcgagg aaacccacgg cggcaatgct gctgcatcac ctcgtggcgc
     5221 agatgggcca ccagaacgcc gtggtggtca gccagaagac actttccaag ctcatcggac
     5281 gttctttgcg gacggtccaa tacgcagtca aggacttggt ggccgagcgc tggatctccg
     5341 tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt ggtcaatgac cgcgtggcgt
     5401 ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag tgccgccgtg gtggttgatc
     5461 acgacgacca ggacgaatcg ctgttggggc atggcgacct gcgccgcatc ccgaccctgt
     5521 atccgggcga gcagcaacta ccgaccggcc ccggcgagga gccgcccagc cagcccggca
     5581 ttccgggcat ggaaccagac ctgccagcct tgaccgaaac ggaggaatgg gaacggcgcg
     5641 ggcagcagcg cctgccgatg cccgatgagc cgtgttttct ggacgatggc gagccgttgg
     5701 agccgccgac acgggtcacg ctgccgcgcc ggtagcactt gggttgcgca gcaacccgta
     5761 agtgcgctgt tccagactat cggctgtagc cgcctcgccg ccctatacct tgtctgcctc
     5821 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     5881 acctcgctaa cggattcacc gtttttatca ggctctggga ggcagaataa atgatcatat
     5941 cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc
     6001 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
     6061 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
     6121 attcatccgc ttattatcac ttattcaggc gtagca
//
