LOCUS       V009259                 5913 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009259
VERSION     V009259
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Repurposing type III polyketide synthase as a malonyl-CoA biosensor
            for metabolic engineering in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   30232266
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Novel Enzyme-coupled Malonyl-CoA Biosensor based on Type III
            Polyketide Synthase and Use of the same (KR 10-2018-0066323)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5913)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   4  (bases 1 to 5913)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5913)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..31
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    39..55
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             76..1305
                     /gene="serA"
                     /label="D-3-phosphoglycerate dehydrogenase"
                     /note="D-3-phosphoglycerate dehydrogenase from Escherichia
                     coli (strain K12). Accession#: P0A9T0"
     misc_feature    1311..1366
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      1569..1655
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1747..1774
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    complement(1795..1816)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1930..2562)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2563..2665)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(2815..3834)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label="mob"
                     /protein_id="AYD60193.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(2815..3834)
                     /gene="mob"
                     /label="mob"
     rep_origin      4058..4829
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             4830..5489
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 gttgacaatt aatcatccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agaccatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
      121 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
      181 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
      241 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaaa
      301 ctggtcgcta ttggctgttt ctgtatcgga acaaaccagg ttgatctgga tgcggcggca
      361 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
      421 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
      481 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
      541 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
      601 tatgtttact tttatgatat tgaaaataaa ctgccgctgg gcaacgccac tcaggtacag
      661 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
      721 tccaccaaaa atatgatggg cgcgaaagaa atttcactaa tgaagcccgg ctcgctgctg
      781 attaatgctt cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
      841 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
      901 ccatttacct ctccgctgtg tgaattcgac aacgtccttc tgacgccaca cattggcggt
      961 tcgactcagg aagcgcagga gaatatcggc ctggaagttg cgggtaaatt gatcaagtat
     1021 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcac
     1081 ggtgggcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
     1141 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctgcaaac ttccgcccag
     1201 atgggttatg tggttattga tattgaagcc gacgaagacg ttgccgaaaa agcgctgcag
     1261 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatg gatccgagct
     1321 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg
     1381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     1441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     1501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1621 gtttgtcggt gaacgctctc ctgagtagga caaatccgtc gggagcggat ttgaacgttg
     1681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     1741 ttaagcagaa ggccatcctg acggatggcc ttttactagt gcctggggtg cctaatgagt
     1801 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1861 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1921 catgcataaa aactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1981 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     2041 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     2101 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     2161 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     2221 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     2281 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     2341 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     2401 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     2461 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     2521 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     2581 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     2641 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt
     2701 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     2761 tatttattcg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2821 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc gcccgcgttc
     2881 ctgctggcgc tgggcctgtt tctggcgctg gacttcccgc tgttccgtca gcagcttttc
     2941 gcccacggcc ttgatgatcg cggcggcctt ggcctgcata tcccgattca acggccccag
     3001 ggcgtccaga acgggcttca ggcgctcccg aaggtctcgg gccgtctctt gggcttgatc
     3061 ggccttcttg cgcatctcac gcgctcctgc ggcggcctgt agggcaggct catacccctg
     3121 ccgaaccgct tttgtcagcc ggtcggccac ggcttccggc gtctcaacgc gctttgagat
     3181 tcccagcttt tcggccaatc cctgcggtgc ataggcgcgt ggctcgaccg cttgcgggct
     3241 gatggtgacg tggcccactg gtggccgctc cagggcctcg tagaacgcct gaatgcgcgt
     3301 gtgacgtgcc ttgctgccct cgatgccccg ttgcagccct agatcggcca cagcggccgc
     3361 aaacgtggtc tggtcgcggg tcatctgcgc tttgttgccg atgaactcct tggccgacag
     3421 cctgccgtcc tgcgtcagcg gcaccacgaa cgcggtcatg tgcgggctgg tttcgtcacg
     3481 gtggatgctg gccgtcacga tgcgatccgc cccgtacttg tccgccagcc acttgtgcgc
     3541 cttctcgaag aacgccgcct gctgttcttg gctggccgac ttccaccatt ccgggctggc
     3601 cgtcatgacg tactcgaccg ccaacacagc gtccttgcgc cgcttctctg gcagcaactc
     3661 gcgcagtcgg cccatcgctt catcggtgct gctggccgcc cagtgctcgt tctctggcgt
     3721 cctgctggcg tcagcgttgg gcgtctcgcg ctcgcggtag gcgtgcttga gactggccgc
     3781 cacgttgccc attttcgcca gcttcttgca tcgcatgatc gcgtatgccg ccatgcctgc
     3841 ccctcccttt tggtgtccaa ccggctcgac gggggcagcg caaggcggtg cctccggcgg
     3901 gccactcaat gcttgagtat actcactaga ctttgcttcg caaagtcgtg accgcctacg
     3961 gcggctgcgg cgccctacgg gcttgctctc cgggcttcgc cctgcgcggt cgctgcgctc
     4021 ccttgccagc ccgtggatat gtggacgatg gccgcgagcg gccaccggct ggctcgcttc
     4081 gctcggcccg tggacaaccc tgctggacaa gctgatggac aggctgcgcc tgcccacgag
     4141 cttgaccaca gggattgccc accggctacc cagccttcga ccacataccc accggctcca
     4201 actgcgcggc ctgcggcctt gccccatcaa tttttttaat tttctctggg gaaaagcctc
     4261 cggcctgcgg cctgcgcgct tcgcttgccg gttggacacc aagtggaagg cgggtcaagg
     4321 ctcgcgcagc gaccgcgcag cggcttggcc ttgacgcgcc tggaacgacc caagcctatg
     4381 cgagtggggg cagtcgaagg gcgaagcccg cccgcctgcc ccccgagcct cacggcggcg
     4441 agtgcggggg ttccaagggg gcagcgccac cttgggcaag gccgaaggcc gcgcagtcga
     4501 tcaacaagcc ccggaggggc cactttttgc cggaggggga gccgcgccga aggcgtgggg
     4561 gaaccccgca ggggtgccct tctttgggca ccaaagaact agatataggg cgaaatgcga
     4621 aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca acagctcatt gcggcacccc
     4681 ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat tgactgccac ttttacgcaa
     4741 cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat tgcgcatggt gccgcaaccg
     4801 tgcggcaccc ctaccgcatg gagataagca tggccacgca gtccagagaa atcggcattc
     4861 aagccaagaa caagcccggt cactgggtgc aaacggaacg caaagcgcat gaggcgtggg
     4921 ccgggcttat tgcgaggaaa cccacggcgg caatgctgct gcatcacctc gtggcgcaga
     4981 tgggccacca gaacgccgtg gtggtcagcc agaagacact ttccaagctc atcggacgtt
     5041 ctttgcggac ggtccaatac gcagtcaagg acttggtggc cgagcgctgg atctccgtcg
     5101 tgaagctcaa cggccccggc accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg
     5161 gccagccccg cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg
     5221 acgaccagga cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg accctgtatc
     5281 cgggcgagca gcaactaccg accggccccg gcgaggagcc gcccagccag cccggcattc
     5341 cgggcatgga accagacctg ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc
     5401 agcagcgcct gccgatgccc gatgagccgt gttttctgga cgatggcgag ccgttggagc
     5461 cgccgacacg ggtcacgctg ccgcgccggt agcacttggg ttgcgcagca acccgtaagt
     5521 gcgctgttcc agactatcgg ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc
     5581 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     5641 tcgctaacgg attcaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt
     5701 caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca
     5761 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
     5821 tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt
     5881 catccgctta ttatcactta ttcaggcgta gca
//
