LOCUS       V009260                 6234 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009260
VERSION     V009260
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 6234)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             1793..2452
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     promoter        3171..3199
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3207..3223
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3242..4357
                     /gene="rppA"
                     /label="1,3,6,8-tetrahydroxynaphthalene synthase"
                     /note="1,3,6,8-tetrahydroxynaphthalene synthase from
                     Streptomyces griseus. Accession#: Q54240"
     regulatory      4404..4550
                     /label="rrnBT1T2"
                     /note="rrnBT1T2"
                     /regulatory_class="terminator"
     protein_bind    complement(4557..4578)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4986..5777)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttgact
     3001 gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc
     3061 aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt
     3121 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta
     3181 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3241 aatggcgacc ctgtgccgac cggccatcgc tgtgcccgag cacgtcatca cgatgcagca
     3301 gaccctggac ctggcccggg agacccatgc cgggcacccg cagcgcgacc tcgtcctgag
     3361 gctcatccag aacaccggcg tccagacccg gcacctcgtg cagcccatcg agaagaccct
     3421 ggcgcacccc ggattcgagg tgcgcaacca ggtgtacgag gccgaggcca agacccgggt
     3481 ccccgaagtc gtccggcggg cgctcgccaa cgccgagacc gagccgtccg agatcgacct
     3541 gatcgtctac gtctcctgca cgggtttcat gatgccctcg ctgaccgcgt ggatcatcaa
     3601 cagcatgggc ttccggcccg agacccgcca actgcccatc gcccagctcg gctgtgcggc
     3661 gggcggcgcg gcgatcaacc gcgcgcacga cttctgcgtg gcctaccccg actccaacgt
     3721 cctcatcgtg tcctgcgagt tctgctcgct gtgctaccag cccaccgaca tcggggtcgg
     3781 ttccctgctc tccaacggac tcttcggcga cgcgctctcc gcggccgtcg tacggggaca
     3841 gggcggcacc ggcatgcgcc tggagcgcaa cggctcccac ctggtgcccg acaccgagga
     3901 ctggatctcc tacgcggtcc gcgacaccgg gttccacttc cagctggaca agcgggtccc
     3961 gggcaccatg gagatgctcg ccccggtgct cctggacctg gtcgacctgc acggctggtc
     4021 cgtcccgaac atggacttct tcatcgtcca cgcgggcgga ccgcgcatcc tggacgacct
     4081 ctgccacttc ctcgacctgc cgcccgagat gttccgctac agccgggcca ccctcaccga
     4141 acgcggcaac atcgcgagct ccgtcgtctt cgacgcgctg gcgcgcctct tcgacgacgg
     4201 cggcgccgcc gagtccgcgc aggggctcat cgccggcttc ggtcccggca tcaccgccga
     4261 ggtggccgtg gggagttggg ccaaggaagg cctcggggcg gacgtcggac gcgacctcga
     4321 cgagctggag ctgaccgccg gcgttgcgct gtccggctaa tggtacccgg ggatcctcta
     4381 gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc
     4441 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     4501 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc tgcctaatga
     4561 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4621 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4681 cgcatgcata aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     4741 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     4801 ttgcccatgg gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     4861 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     4921 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     4981 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     5041 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     5101 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     5161 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     5221 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     5281 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     5341 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     5401 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     5461 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5521 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5581 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     5641 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     5701 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     5761 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
     5821 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
     5881 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc
     5941 cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg
     6001 ttttcccttg tccagatagc ccagtagctg acattcatcc caggtggcac ttttcgggga
     6061 aatgtgcgcg cccgcgttcc tgctggcgct gggcctgttt ctggcgctgg acttcccgct
     6121 gttccgtcag cagcttttcg cccacggcct tgatgatcgc ggcggccttg gcctgcatat
     6181 cccgattcaa cggccccagg gcgtccagaa cgggcttcag gcgctcccga aggt
//
