LOCUS       V009261                 5833 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009261
VERSION     V009261
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Repurposing type III polyketide synthase as a malonyl-CoA biosensor
            for metabolic engineering in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   30232266
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Novel Enzyme-coupled Malonyl-CoA Biosensor based on Type III
            Polyketide Synthase and Use of the same (KR 10-2018-0066323)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5833)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   4  (bases 1 to 5833)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..30
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    38..54
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             77..1237
                     /gene="pgk"
                     /label="Phosphoglycerate kinase"
                     /note="Phosphoglycerate kinase from Escherichia coli
                     (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 /
                     WDCM 00012 / Crooks). Accession#: B1IT77"
     terminator      1489..1575
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1667..1694
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    complement(1715..1736)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1850..2482)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2483..2585)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(2735..3754)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label="mob"
                     /protein_id="AYD60206.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(2735..3754)
                     /gene="mob"
                     /label="mob"
     rep_origin      3978..4749
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             4750..5409
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gaattcatgt ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg
      121 tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc
      181 gcgtatccgt gcttctctgc cgaccatcga actggccctg aaacaaggcg caaaagtgat
      241 ggtaacttcc cacctgggtc gtcctaccga aggcgagtac aacgaagaat tctctctgct
      301 gccggttgtt aactacctga aagacaaact gtctaacccg gttcgtctgg ttaaagatta
      361 cctcgacggc gttgacgttg ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa
      421 caaaggcgag aagaaagacg acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt
      481 gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag gcttctactc acggtatcgg
      541 taaattcgct gacgttgcgt gcgcaggccc gctgctggca gctgaactgg acgcgctggg
      601 taaagcactg aaagaacctg ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc
      661 taccaaactg accgttctgg actccctgtc taaaatcgct gaccagctga ttgttggtgg
      721 tggtatcgct aacaccttta tcgcggcaca aggccacgat gtgggtaaat ccctgtacga
      781 agctgacctg gttgacgaag ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc
      841 tgatgttcgc gtagcaaccg agttctctga aactgcaccg gctaccctga aatctgttaa
      901 cgatgtgaaa gctgacgagc agatcctgga tatcggtgat gcttccgctc aggaactggc
      961 tgaaatcctg aagaatgcga aaaccattct gtggaacggt ccggttggcg tgttcgaatt
     1021 cccgaacttc cgcaaaggta ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt
     1081 ctccatcgct ggcggcggcg acactctggc agcaatcgac ctgttcggca ttgctgacaa
     1141 aatctcctac atctccactg gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact
     1201 gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa tggtacccgg ggatcctcta
     1261 gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc
     1321 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     1381 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     1441 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     1501 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1561 ctgagtagga caaatccgtc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     1621 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     1681 acggatggcc ttttactagt gcctggggtg cctaatgagt gagctaactc acattaattg
     1741 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg catgcataaa aactgttgta
     1861 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1921 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1981 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     2041 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     2101 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     2161 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     2221 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     2281 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     2341 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     2401 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2461 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     2521 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     2581 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     2641 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg aagacgaaag
     2701 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2761 tcaggtggca cttttcgggg aaatgtgcgc gcccgcgttc ctgctggcgc tgggcctgtt
     2821 tctggcgctg gacttcccgc tgttccgtca gcagcttttc gcccacggcc ttgatgatcg
     2881 cggcggcctt ggcctgcata tcccgattca acggccccag ggcgtccaga acgggcttca
     2941 ggcgctcccg aaggtctcgg gccgtctctt gggcttgatc ggccttcttg cgcatctcac
     3001 gcgctcctgc ggcggcctgt agggcaggct catacccctg ccgaaccgct tttgtcagcc
     3061 ggtcggccac ggcttccggc gtctcaacgc gctttgagat tcccagcttt tcggccaatc
     3121 cctgcggtgc ataggcgcgt ggctcgaccg cttgcgggct gatggtgacg tggcccactg
     3181 gtggccgctc cagggcctcg tagaacgcct gaatgcgcgt gtgacgtgcc ttgctgccct
     3241 cgatgccccg ttgcagccct agatcggcca cagcggccgc aaacgtggtc tggtcgcggg
     3301 tcatctgcgc tttgttgccg atgaactcct tggccgacag cctgccgtcc tgcgtcagcg
     3361 gcaccacgaa cgcggtcatg tgcgggctgg tttcgtcacg gtggatgctg gccgtcacga
     3421 tgcgatccgc cccgtacttg tccgccagcc acttgtgcgc cttctcgaag aacgccgcct
     3481 gctgttcttg gctggccgac ttccaccatt ccgggctggc cgtcatgacg tactcgaccg
     3541 ccaacacagc gtccttgcgc cgcttctctg gcagcaactc gcgcagtcgg cccatcgctt
     3601 catcggtgct gctggccgcc cagtgctcgt tctctggcgt cctgctggcg tcagcgttgg
     3661 gcgtctcgcg ctcgcggtag gcgtgcttga gactggccgc cacgttgccc attttcgcca
     3721 gcttcttgca tcgcatgatc gcgtatgccg ccatgcctgc ccctcccttt tggtgtccaa
     3781 ccggctcgac gggggcagcg caaggcggtg cctccggcgg gccactcaat gcttgagtat
     3841 actcactaga ctttgcttcg caaagtcgtg accgcctacg gcggctgcgg cgccctacgg
     3901 gcttgctctc cgggcttcgc cctgcgcggt cgctgcgctc ccttgccagc ccgtggatat
     3961 gtggacgatg gccgcgagcg gccaccggct ggctcgcttc gctcggcccg tggacaaccc
     4021 tgctggacaa gctgatggac aggctgcgcc tgcccacgag cttgaccaca gggattgccc
     4081 accggctacc cagccttcga ccacataccc accggctcca actgcgcggc ctgcggcctt
     4141 gccccatcaa tttttttaat tttctctggg gaaaagcctc cggcctgcgg cctgcgcgct
     4201 tcgcttgccg gttggacacc aagtggaagg cgggtcaagg ctcgcgcagc gaccgcgcag
     4261 cggcttggcc ttgacgcgcc tggaacgacc caagcctatg cgagtggggg cagtcgaagg
     4321 gcgaagcccg cccgcctgcc ccccgagcct cacggcggcg agtgcggggg ttccaagggg
     4381 gcagcgccac cttgggcaag gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc
     4441 cactttttgc cggaggggga gccgcgccga aggcgtgggg gaaccccgca ggggtgccct
     4501 tctttgggca ccaaagaact agatataggg cgaaatgcga aagacttaaa aatcaacaac
     4561 ttaaaaaagg ggggtacgca acagctcatt gcggcacccc ccgcaatagc tcattgcgta
     4621 ggttaaagaa aatctgtaat tgactgccac ttttacgcaa cgcataattg ttgtcgcgct
     4681 gccgaaaagt tgcagctgat tgcgcatggt gccgcaaccg tgcggcaccc ctaccgcatg
     4741 gagataagca tggccacgca gtccagagaa atcggcattc aagccaagaa caagcccggt
     4801 cactgggtgc aaacggaacg caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa
     4861 cccacggcgg caatgctgct gcatcacctc gtggcgcaga tgggccacca gaacgccgtg
     4921 gtggtcagcc agaagacact ttccaagctc atcggacgtt ctttgcggac ggtccaatac
     4981 gcagtcaagg acttggtggc cgagcgctgg atctccgtcg tgaagctcaa cggccccggc
     5041 accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg gccagccccg cgaccagttg
     5101 cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg acgaccagga cgaatcgctg
     5161 ttggggcatg gcgacctgcg ccgcatcccg accctgtatc cgggcgagca gcaactaccg
     5221 accggccccg gcgaggagcc gcccagccag cccggcattc cgggcatgga accagacctg
     5281 ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc
     5341 gatgagccgt gttttctgga cgatggcgag ccgttggagc cgccgacacg ggtcacgctg
     5401 ccgcgccggt agcacttggg ttgcgcagca acccgtaagt gcgctgttcc agactatcgg
     5461 ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     5521 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccgtt
     5581 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg
     5641 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta
     5701 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
     5761 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
     5821 ttcaggcgta gca
//
