LOCUS       V009265                 5665 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009265
VERSION     V009265
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Repurposing type III polyketide synthase as a malonyl-CoA biosensor
            for metabolic engineering in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   30232266
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Novel Enzyme-coupled Malonyl-CoA Biosensor based on Type III
            Polyketide Synthase and Use of the same (KR 10-2018-0066323)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5665)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   4  (bases 1 to 5665)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5665)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..30
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    38..54
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             77..1069
                     /gene="gapA"
                     /label="Glyceraldehyde-3-phosphate dehydrogenase A"
                     /note="Glyceraldehyde-3-phosphate dehydrogenase A from
                     Escherichia coli (strain K12). Accession#: P0A9B2"
     terminator      1321..1407
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1499..1526
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    complement(1547..1568)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1682..2314)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2315..2417)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(2567..3586)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label="mob"
                     /protein_id="AYD60202.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(2567..3586)
                     /gene="mob"
                     /label="mob"
     rep_origin      3810..4581
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             4582..5241
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gaattcatga ctatcaaagt aggtatcaac ggttttggcc gtatcggtcg
      121 cattgttttc cgtgctgctc agaaacgttc tgacatcgag atcgttgcaa tcaacgacct
      181 gttagacgct gattacatgg catacatgct gaaatatgac tccactcacg gccgtttcga
      241 cggtaccgtt gaagtgaaag acggtcatct gatcgttaac ggtaaaaaaa tccgtgttac
      301 cgctgaacgt gatccggcta acctgaaatg ggacgaagtt ggtgttgacg ttgtcgctga
      361 agcaactggt ctgttcctga ctgacgaaac tgctcgtaaa cacatcaccg ctggtgcgaa
      421 gaaagtggtt atgactggtc cgtctaaaga caacactccg atgttcgtta aaggcgctaa
      481 cttcgacaaa tatgctggcc aggacatcgt ttccaacgct tcctgcacca ccaactgcct
      541 ggctccgctg gctaaagtta tcaacgataa cttcggcatc atcgaaggtc tgatgaccac
      601 cgttcacgct actaccgcta ctcagaaaac cgttgatggc ccgtctcaca aagactggcg
      661 cggcggccgc ggcgcttccc agaacatcat cccgtcctct accggtgctg ctaaagctgt
      721 aggtaaagta ctgccagaac tgaatggcaa actgactggt atggcgttcc gcgttccgac
      781 cccgaacgta tctgtagttg acctgaccgt tcgtctggaa aaagctgcaa cttacgagca
      841 gatcaaagct gccgttaaag ctgctgctga aggcgaaatg aaaggcgttc tgggctacac
      901 cgaagatgac gtagtatcta ccgatttcaa cggcgaagtt tgcacttccg tgttcgatgc
      961 taaagctggt atcgctctga acgacaactt cgtgaaactg gtatcctggt acgacaacga
     1021 aaccggttac tccaacaaag ttctggacct gatcgctcac atctccaaat aatggtaccc
     1081 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg ctgttttggc ggatgagaga
     1141 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     1201 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     1261 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     1321 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1381 gtgaacgctc tcctgagtag gacaaatccg tcgggagcgg atttgaacgt tgcgaagcaa
     1441 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     1501 aaggccatcc tgacggatgg ccttttacta gtgcctgggg tgcctaatga gtgagctaac
     1561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
     1681 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1741 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1801 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1861 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1921 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1981 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2041 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt
     2101 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2161 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2221 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2281 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2341 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2401 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
     2461 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt
     2521 cgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2581 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc
     2641 gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg
     2701 ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca
     2761 gaacgggctt caggcgctcc cgaaggtctc gggccgtctc ttgggcttga tcggccttct
     2821 tgcgcatctc acgcgctcct gcggcggcct gtagggcagg ctcatacccc tgccgaaccg
     2881 cttttgtcag ccggtcggcc acggcttccg gcgtctcaac gcgctttgag attcccagct
     2941 tttcggccaa tccctgcggt gcataggcgc gtggctcgac cgcttgcggg ctgatggtga
     3001 cgtggcccac tggtggccgc tccagggcct cgtagaacgc ctgaatgcgc gtgtgacgtg
     3061 ccttgctgcc ctcgatgccc cgttgcagcc ctagatcggc cacagcggcc gcaaacgtgg
     3121 tctggtcgcg ggtcatctgc gctttgttgc cgatgaactc cttggccgac agcctgccgt
     3181 cctgcgtcag cggcaccacg aacgcggtca tgtgcgggct ggtttcgtca cggtggatgc
     3241 tggccgtcac gatgcgatcc gccccgtact tgtccgccag ccacttgtgc gccttctcga
     3301 agaacgccgc ctgctgttct tggctggccg acttccacca ttccgggctg gccgtcatga
     3361 cgtactcgac cgccaacaca gcgtccttgc gccgcttctc tggcagcaac tcgcgcagtc
     3421 ggcccatcgc ttcatcggtg ctgctggccg cccagtgctc gttctctggc gtcctgctgg
     3481 cgtcagcgtt gggcgtctcg cgctcgcggt aggcgtgctt gagactggcc gccacgttgc
     3541 ccattttcgc cagcttcttg catcgcatga tcgcgtatgc cgccatgcct gcccctccct
     3601 tttggtgtcc aaccggctcg acgggggcag cgcaaggcgg tgcctccggc gggccactca
     3661 atgcttgagt atactcacta gactttgctt cgcaaagtcg tgaccgccta cggcggctgc
     3721 ggcgccctac gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca
     3781 gcccgtggat atgtggacga tggccgcgag cggccaccgg ctggctcgct tcgctcggcc
     3841 cgtggacaac cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca
     3901 cagggattgc ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg
     3961 gcctgcggcc ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc
     4021 ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca
     4081 gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg
     4141 ggcagtcgaa gggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg
     4201 ggttccaagg gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag
     4261 ccccggaggg gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg
     4321 caggggtgcc cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta
     4381 aaaatcaaca acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata
     4441 gctcattgcg taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat
     4501 tgttgtcgcg ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac
     4561 ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat tcaagccaag
     4621 aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg ggccgggctt
     4681 attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca gatgggccac
     4741 cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg ttctttgcgg
     4801 acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt cgtgaagctc
     4861 aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg gggccagccc
     4921 cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca cgacgaccag
     4981 gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta tccgggcgag
     5041 cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat tccgggcatg
     5101 gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg gcagcagcgc
     5161 ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga gccgccgaca
     5221 cgggtcacgc tgccgcgccg gtagcacttg ggttgcgcag caacccgtaa gtgcgctgtt
     5281 ccagactatc ggctgtagcc gcctcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     5341 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
     5401 ggattcaccg tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt
     5461 acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca
     5521 ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga
     5581 ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct
     5641 tattatcact tattcaggcg tagca
//
