LOCUS       V009266                 6628 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009266
VERSION     V009266
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Repurposing type III polyketide synthase as a malonyl-CoA biosensor
            for metabolic engineering in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   30232266
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Novel Enzyme-coupled Malonyl-CoA Biosensor based on Type III
            Polyketide Synthase and Use of the same (KR 10-2018-0066323)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6628)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   4  (bases 1 to 6628)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6628)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..30
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    38..54
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             77..2032
                     /gene="acs"
                     /label="Acetyl-coenzyme A synthetase"
                     /note="Acetyl-coenzyme A synthetase from Escherichia coli
                     (strain K12). Accession#: P27550"
     terminator      2284..2370
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2462..2489
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    complement(2510..2531)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2645..3277)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3278..3380)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(3530..4549)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label="mob"
                     /protein_id="AYD60210.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(3530..4549)
                     /gene="mob"
                     /label="mob"
     rep_origin      4773..5544
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             5545..6204
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gaattcatga gccaaattca caaacacacc attcctgcca acatcgcaga
      121 ccgttgcctg ataaaccctc agcagtacga ggcgatgtat caacaatcta ttaacgtacc
      181 tgataccttc tggggcgaac agggaaaaat tcttgactgg atcaaacctt accagaaggt
      241 gaaaaacacc tcctttgccc ccggtaatgt gtccattaaa tggtacgagg acggcacgct
      301 gaatctggcg gcaaactgcc ttgaccgcca tctgcaagaa aacggcgatc gtaccgccat
      361 catctgggaa ggcgacgacg ccagccagag caaacatatc agctataaag agctgcaccg
      421 cgacgtctgc cgcttcgcca ataccctgct cgagctgggc attaaaaaag gtgatgtggt
      481 ggcgatttat atgccgatgg tgccggaagc cgcggttgcg atgctggcct gcgcccgcat
      541 tggcgcggtg cattcggtga ttttcggcgg cttctcgccg gaagccgttg ccgggcgcat
      601 tattgattcc aactcacgac tggtgatcac ttccgacgaa ggtgtgcgtg ccgggcgcag
      661 tattccgctg aagaaaaacg ttgatgacgc gctgaaaaac ccgaacgtca ccagcgtaga
      721 gcatgtggtg gtactgaagc gtactggcgg gaaaattgac tggcaggaag ggcgcgacct
      781 gtggtggcac gacctggttg agcaagcgag cgatcagcac caggcggaag agatgaacgc
      841 cgaagatccg ctgtttattc tctacacctc cggttctacc ggtaagccaa aaggtgtgct
      901 gcatactacc ggcggttatc tggtgtacgc ggcgctgacc tttaaatatg tctttgatta
      961 tcatccgggt gatatctact ggtgcaccgc cgatgtgggc tgggtgaccg gacacagtta
     1021 cttgctgtac ggcccgctgg cctgcggtgc gaccacgctg atgtttgaag gcgtaccgaa
     1081 ctggccgacg cctgcccgta tggcgcaggt ggtggacaag catcaggtca atattctcta
     1141 taccgcgccc acggcgattc gcgcgctgat ggcggaaggg gataaagcga ttgaaggcac
     1201 cgaccgatcg tcgctgcgca ttctcggttc cgtgggcgag ccaatcaacc cggaagcgtg
     1261 ggagtggtac tggaaaaaaa tcggcaacga gaaatgtccg gtggtcgata cctggtggca
     1321 gaccgaaact ggcggtttca tgatcacgcc gctgcctggc gctaccgagc tgaaagccgg
     1381 ttcggcaaca cgtccgttct tcggcgtgca accggcgctg gtcgataacg aaggtaaccc
     1441 gctggaaggc gctaccgaag gcagcctggt gatcaccgac tcctggccgg gtcaggcgcg
     1501 tacgctgttt ggcgatcacg aacgttttga acagacctac ttctccacct tcaaaaatat
     1561 gtatttcagc ggcgacggcg cgcgtcgcga tgaagatggc tattactgga taaccgggcg
     1621 tgtggacgat gtgctgaacg tctccggtca ccgtctggga acggcggaga ttgagtcggc
     1681 gctggtggcg catccgaaga ttgccgaagc cgccgtagta ggtattccgc acaatattaa
     1741 aggtcaggcg atctacgcct acgtcacgct taatcacggg gaggaaccgt caccagaact
     1801 gtacgcagaa gtccgcaact gggtgcgtaa agagattggc ccgctggcga cgccagacgt
     1861 gctgcactgg accgactccc tgcctaaaac ccgctccggc aaaattatgc gccgtattct
     1921 gcgcaaaatt gcggcgggcg ataccagcaa cctgggcgat acctcgacgc ttgccgatcc
     1981 tggcgtagtc gagaagctgc ttgaagagaa gcaggctatc gcgatgccat cgtaatggta
     2041 cccggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag
     2101 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2161 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     2221 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     2281 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2341 tcggtgaacg ctctcctgag taggacaaat ccgtcgggag cggatttgaa cgttgcgaag
     2401 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     2461 cagaaggcca tcctgacgga tggcctttta ctagtgcctg gggtgcctaa tgagtgagct
     2521 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2581 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgcatgc
     2641 ataaaaactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2701 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2761 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2821 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     2881 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2941 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     3001 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga
     3061 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     3121 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     3181 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     3241 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     3301 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     3361 aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg
     3421 tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt
     3481 attcgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3541 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcgcccg cgttcctgct
     3601 ggcgctgggc ctgtttctgg cgctggactt cccgctgttc cgtcagcagc ttttcgccca
     3661 cggccttgat gatcgcggcg gccttggcct gcatatcccg attcaacggc cccagggcgt
     3721 ccagaacggg cttcaggcgc tcccgaaggt ctcgggccgt ctcttgggct tgatcggcct
     3781 tcttgcgcat ctcacgcgct cctgcggcgg cctgtagggc aggctcatac ccctgccgaa
     3841 ccgcttttgt cagccggtcg gccacggctt ccggcgtctc aacgcgcttt gagattccca
     3901 gcttttcggc caatccctgc ggtgcatagg cgcgtggctc gaccgcttgc gggctgatgg
     3961 tgacgtggcc cactggtggc cgctccaggg cctcgtagaa cgcctgaatg cgcgtgtgac
     4021 gtgccttgct gccctcgatg ccccgttgca gccctagatc ggccacagcg gccgcaaacg
     4081 tggtctggtc gcgggtcatc tgcgctttgt tgccgatgaa ctccttggcc gacagcctgc
     4141 cgtcctgcgt cagcggcacc acgaacgcgg tcatgtgcgg gctggtttcg tcacggtgga
     4201 tgctggccgt cacgatgcga tccgccccgt acttgtccgc cagccacttg tgcgccttct
     4261 cgaagaacgc cgcctgctgt tcttggctgg ccgacttcca ccattccggg ctggccgtca
     4321 tgacgtactc gaccgccaac acagcgtcct tgcgccgctt ctctggcagc aactcgcgca
     4381 gtcggcccat cgcttcatcg gtgctgctgg ccgcccagtg ctcgttctct ggcgtcctgc
     4441 tggcgtcagc gttgggcgtc tcgcgctcgc ggtaggcgtg cttgagactg gccgccacgt
     4501 tgcccatttt cgccagcttc ttgcatcgca tgatcgcgta tgccgccatg cctgcccctc
     4561 ccttttggtg tccaaccggc tcgacggggg cagcgcaagg cggtgcctcc ggcgggccac
     4621 tcaatgcttg agtatactca ctagactttg cttcgcaaag tcgtgaccgc ctacggcggc
     4681 tgcggcgccc tacgggcttg ctctccgggc ttcgccctgc gcggtcgctg cgctcccttg
     4741 ccagcccgtg gatatgtgga cgatggccgc gagcggccac cggctggctc gcttcgctcg
     4801 gcccgtggac aaccctgctg gacaagctga tggacaggct gcgcctgccc acgagcttga
     4861 ccacagggat tgcccaccgg ctacccagcc ttcgaccaca tacccaccgg ctccaactgc
     4921 gcggcctgcg gccttgcccc atcaattttt ttaattttct ctggggaaaa gcctccggcc
     4981 tgcggcctgc gcgcttcgct tgccggttgg acaccaagtg gaaggcgggt caaggctcgc
     5041 gcagcgaccg cgcagcggct tggccttgac gcgcctggaa cgacccaagc ctatgcgagt
     5101 gggggcagtc gaagggcgaa gcccgcccgc ctgccccccg agcctcacgg cggcgagtgc
     5161 gggggttcca agggggcagc gccaccttgg gcaaggccga aggccgcgca gtcgatcaac
     5221 aagccccgga ggggccactt tttgccggag ggggagccgc gccgaaggcg tgggggaacc
     5281 ccgcaggggt gcccttcttt gggcaccaaa gaactagata tagggcgaaa tgcgaaagac
     5341 ttaaaaatca acaacttaaa aaaggggggt acgcaacagc tcattgcggc accccccgca
     5401 atagctcatt gcgtaggtta aagaaaatct gtaattgact gccactttta cgcaacgcat
     5461 aattgttgtc gcgctgccga aaagttgcag ctgattgcgc atggtgccgc aaccgtgcgg
     5521 cacccctacc gcatggagat aagcatggcc acgcagtcca gagaaatcgg cattcaagcc
     5581 aagaacaagc ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc gtgggccggg
     5641 cttattgcga ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc gcagatgggc
     5701 caccagaacg ccgtggtggt cagccagaag acactttcca agctcatcgg acgttctttg
     5761 cggacggtcc aatacgcagt caaggacttg gtggccgagc gctggatctc cgtcgtgaag
     5821 ctcaacggcc ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc gtggggccag
     5881 ccccgcgacc agttgcgcct gtcggtgttc agtgccgccg tggtggttga tcacgacgac
     5941 caggacgaat cgctgttggg gcatggcgac ctgcgccgca tcccgaccct gtatccgggc
     6001 gagcagcaac taccgaccgg ccccggcgag gagccgccca gccagcccgg cattccgggc
     6061 atggaaccag acctgccagc cttgaccgaa acggaggaat gggaacggcg cgggcagcag
     6121 cgcctgccga tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt ggagccgccg
     6181 acacgggtca cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg taagtgcgct
     6241 gttccagact atcggctgta gccgcctcgc cgccctatac cttgtctgcc tccccgcgtt
     6301 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     6361 aacggattca ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt
     6421 attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc
     6481 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     6541 cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc
     6601 gcttattatc acttattcag gcgtagca
//
