LOCUS       V009267                10763 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009267
VERSION     V009267
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Repurposing type III polyketide synthase as a malonyl-CoA biosensor
            for metabolic engineering in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   30232266
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Novel Enzyme-coupled Malonyl-CoA Biosensor based on Type III
            Polyketide Synthase and Use of the same (KR 10-2018-0066323)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10763)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   4  (bases 1 to 10763)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10763)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..30
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    38..54
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             77..2737
                     /gene="aceE"
                     /label="Pyruvate dehydrogenase E1 component"
                     /note="Pyruvate dehydrogenase E1 component from Escherichia
                     coli O157:H7. Accession#: P0AFG9"
     CDS             2755..4644
                     /gene="aceF"
                     /label="Dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex"
                     /note="Dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex from
                     Escherichia coli (strain K12). Accession#: P06959"
     regulatory      4671..4699
                     /label="Tac promoter"
                     /note="Tac promoter"
                     /regulatory_class="promoter"
     promoter        4671..4699
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4707..4723
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4746..6167
                     /gene="lpdA"
                     /label="Dihydrolipoyl dehydrogenase"
                     /note="Dihydrolipoyl dehydrogenase from Escherichia coli
                     O6:H1 (strain CFT073 / ATCC 700928 / UPEC). Accession#:
                     P0A9P1"
     terminator      6419..6505
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6597..6624
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    complement(6645..6666)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(6780..7412)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7413..7515)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(7665..8684)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label="mob"
                     /protein_id="AYD60217.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(7665..8684)
                     /gene="mob"
                     /label="mob"
     rep_origin      8908..9679
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             9680..10339
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
ORIGIN
        1 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gaattcatgt cagaacgttt cccaaatgac gtggatccga tcgaaactcg
      121 cgactggctc caggcgatcg aatcggtcat ccgtgaagaa ggtgttgagc gtgctcagta
      181 tctgatcgac caactgcttg ctgaagcccg caaaggcggt gtaaacgtag ccgcaggcac
      241 aggtatcagc aactacatca acaccatccc cgttgaagaa caaccggagt atccgggtaa
      301 tctggaactg gaacgccgta ttcgttcagc tatccgctgg aacgccatca tgacggtgct
      361 gcgtgcgtcg aaaaaagacc tcgaactggg cggccatatg gcgtccttcc agtcttccgc
      421 aaccatttat gatgtgtgct ttaaccactt cttccgtgca cgcaacgagc aggatggcgg
      481 cgacctggtt tacttccagg gccacatctc cccgggcgtg tacgctcgtg ctttcctgga
      541 aggtcgtctg actcaggagc agctggataa cttccgtcag gaagttcacg gcaatggcct
      601 ctcttcctat ccgcacccga aactgatgcc ggaattctgg cagttcccga ccgtatctat
      661 gggtctgggg ccgattggtg ctatttacca ggctaaattc ctgaaatatc tggaacaccg
      721 tggcctgaaa gatacctcta aacagaccgt ttacgcgttc ctcggcgacg gtgaaatgga
      781 cgaaccggaa tccaaaggtg cgatcaccat cgctacccgt gaaaaactgg ataacctggt
      841 cttcgttatc aactgtaacc tgcagcgtct tgacggcccg gtcaccggta acggcaagat
      901 catcaacgaa ctggaaggca tcttcgaagg tgctggctgg aacgtgatca aagtgatgtg
      961 gggtagccgt tgggatgaac tgctgcgtaa agataccagc ggtaaactga tccagctgat
     1021 gaacgaaacc gttgacggcg actaccagac cttcaaatcg aaagatggtg cgtacgttcg
     1081 tgaacacttc ttcggtaaat atcctgaaac cgcagcactg gttgcagact ggactgacga
     1141 gcagatctgg gcactgaacc gtggtggtca cgatccgaag aaaatctacg ctgcattcaa
     1201 gaaagcgcag gaaaccaaag gcaaagcgac agtaatcctt gctcatacca ttaaaggtta
     1261 cggcatgggc gacgcggctg aaggtaaaaa catcgcgcac caggttaaga aaatgaacat
     1321 ggacggcgtg cgtcacatcc gcgaccgttt caatgtgccg gtgtctgata cagatatcga
     1381 aaaactgccg tacatcacct tcccggaagg ttctgaagag catacctatc tgcacgctca
     1441 gcgtcagaaa ctgcacggtt atctgccaag ccgtcagccg aacttcaccg agaagcttga
     1501 gctgccgagc ctgcaagact tcggcgcgct gttggaagag cagagcaaag agatctctac
     1561 cactatcgct ttcgttcgtg ctctgaacgt gatgctgaag aacaagtcga tcaaagatcg
     1621 tctggtaccg atcatcgccg acgaagcgcg tactttcggt atggaaggtc tgttccgtca
     1681 gattggtatt tacagcccga acggtcagca gtacaccccg caggaccgcg agcaggttgc
     1741 ttactataaa gaagacgaga aaggtcagat tctgcaggaa gggatcaacg agctgggcgc
     1801 aggttgttcc tggctggcag cggcgacctc ttacagcacc aacaatctgc cgatgatccc
     1861 gttctacatc tattactcga tgttcggctt ccagcgtatt ggcgatctgt gctgggcggc
     1921 tggcgaccag caagcgcgtg gcttcctgat cggcggtact tccggtcgta ccaccctgaa
     1981 cggcgaaggt ctgcagcacg aagatggtca cagccacatt cagtcgctga ctatcccgaa
     2041 ctgtatctct tacgacccgg cttacgctta cgaagttgct gtcatcatgc atgacggtct
     2101 ggagcgtatg tacggtgaaa aacaagagaa cgtttactac tacatcacta cgctgaacga
     2161 aaactaccac atgccggcaa tgccggaagg tgctgaggaa ggtatccgta aaggtatcta
     2221 caaactcgaa actattgaag gtagcaaagg taaagttcag ctgctcggct ccggttctat
     2281 cctgcgtcac gtccgtgaag cagctgagat cctggcgaaa gattacggcg taggttctga
     2341 cgtttatagc gtgacctcct tcaccgagct ggcgcgtgat ggtcaggatt gtgaacgctg
     2401 gaacatgctg cacccgctgg aaactccgcg cgttccgtat atcgctcagg tgatgaacga
     2461 cgctccggca gtggcatcta ccgactatat gaaactgttc gctgagcagg tccgtactta
     2521 cgtaccggct gacgactacc gcgtactggg tactgatggc ttcggtcgtt ccgacagccg
     2581 tgagaacctg cgtcaccact tcgaagttga tgcttcttat gtcgtggttg cggcgctggg
     2641 cgaactggct aaacgtggcg aaatcgataa gaaagtggtt gctgacgcaa tcgccaaatt
     2701 caacatcgat gcagataaag ttaacccgcg tctggcgtaa gaggtaaaag aataatggct
     2761 atcgaaatca aagtaccgga catcggggct gatgaagttg aaatcaccga gatcctggtc
     2821 aaagtgggcg acaaagttga agccgaacag tcgctgatca ccgtagaagg cgacaaagcc
     2881 tctatggaag ttccgtctcc gcaggcgggt atcgttaaag agatcaaagt ctctgttggc
     2941 gataaaaccc agaccggcgc actgattatg attttcgatt ccgccgacgg tgcagcagac
     3001 gctgcacctg ctcaggcaga agagaagaaa gaagcagctc cggcagcagc accagcggct
     3061 gcggcggcaa aagacgttaa cgttccggat atcggcagcg acgaagttga agtgaccgaa
     3121 atcctggtga aagttggcga taaagttgaa gctgaacagt cgctgatcac cgtagaaggc
     3181 gacaaggctt ctatggaagt tccggctccg tttgctggca ccgtgaaaga gatcaaagtg
     3241 aacgtgggtg acaaagtgtc taccggctcg ctgattatgg tcttcgaagt cgcgggtgaa
     3301 gcaggcgcgg cagctccggc cgctaaacag gaagcagctc cggcagcggc ccctgcacca
     3361 gcggctggcg tgaaagaagt taacgttccg gatatcggcg gtgacgaagt tgaagtgact
     3421 gaagtgatgg tgaaagtggg cgacaaagtt gccgctgaac agtcactgat caccgtagaa
     3481 ggcgacaaag cttctatgga agttccggcg ccgtttgcag gcgtcgtgaa ggaactgaaa
     3541 gtcaacgttg gcgataaagt gaaaactggc tcgctgatta tgatcttcga agttgaaggc
     3601 gcagcgcctg cggcagctcc tgcgaaacag gaagcggcag cgccggcacc ggcagcaaaa
     3661 gctgaagccc cggcagcagc accagctgcg aaagcggaag gcaaatctga atttgctgaa
     3721 aacgacgctt atgttcacgc gactccgctg atccgccgtc tggcacgcga gtttggtgtt
     3781 aaccttgcga aagtgaaggg cactggccgt aaaggtcgta tcctgcgcga agacgttcag
     3841 gcttacgtga aagaagctat caaacgtgca gaagcagctc cggcagcgac tggcggtggt
     3901 atccctggca tgctgccgtg gccgaaggtg gacttcagca agtttggtga aatcgaagaa
     3961 gtggaactgg gccgcatcca gaaaatctct ggtgcgaacc tgagccgtaa ctgggtaatg
     4021 atcccgcatg ttactcactt cgacaaaacc gatatcaccg agttggaagc gttccgtaaa
     4081 cagcagaacg aagaagcggc gaaacgtaag ctggatgtga agatcacccc ggttgtcttc
     4141 atcatgaaag ccgttgctgc agctcttgag cagatgcctc gcttcaatag ttcgctgtcg
     4201 gaagacggtc agcgtctgac cctgaagaaa tacatcaaca tcggtgtggc ggtggatacc
     4261 ccgaacggtc tggttgttcc ggtattcaaa gacgtcaaca agaaaggcat catcgagctg
     4321 tctcgcgagc tgatgactat ttctaagaaa gcgcgtgacg gtaagctgac tgcgggcgaa
     4381 atgcagggcg gttgcttcac catctccagc atcggcggcc tgggtactac ccacttcgcg
     4441 ccgattgtga acgcgccgga agtggctatc ctcggcgttt ccaagtccgc gatggagccg
     4501 gtgtggaatg gtaaagagtt cgtgccgcgt ctgatgctgc cgatttctct ctccttcgac
     4561 caccgcgtga tcgacggtgc tgatggtgcc cgtttcatta ccatcattaa caacacgctg
     4621 tctgacattc gccgtctggt gatgtaatgg tacccgggga tcctctagag ttgacaatta
     4681 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4741 aattcatgag tactgaaatc aaaactcagg tcgtggtact tggggcaggc cccgcaggtt
     4801 actccgctgc cttccgttgc gctgatttag gtctggaaac cgtaatcgta gaacgttaca
     4861 acacccttgg cggtgtttgc ctgaacgtcg gctgtatccc ttctaaagca ctgctgcacg
     4921 tagcaaaagt tatcgaagaa gccaaagcgc tggctgaaca cggtatcgtc ttcggcgaac
     4981 cgaaaaccga tatcgacaag attcgtacct ggaaagagaa agtgatcaat cagctgaccg
     5041 gtggtctggc tggtatggcg aaaggccgca aagtcaaagt ggtcaacggt ctgggtaaat
     5101 tcaccggggc taacaccctg gaagttgaag gtgagaacgg caaaaccgtg atcaacttcg
     5161 acaacgcgat cattgcagcg ggttctcgcc cgatccaact gccgtttatt ccgcatgaag
     5221 atccgcgtat ctgggactcc actgacgcgc tggaactgaa agaagtacca gaacgcctgc
     5281 tggtaatggg tggcggtatc atcggtctgg aaatgggcac cgtttaccac gcgctgggtt
     5341 cacagattga cgtggttgaa atgttcgacc aggttatccc ggcagctgac aaagacatcg
     5401 ttaaagtctt caccaagcgt atcagcaaga aattcaacct gatgctggaa accaaagtta
     5461 ccgccgttga agcgaaagaa gacggcattt atgtgacgat ggaaggcaaa aaagcacccg
     5521 ctgaaccgca gcgttacgac gccgtgctgg tagcgattgg tcgtgtgccg aacggtaaaa
     5581 acctcgacgc aggcaaagca ggcgtggaag ttgacgaccg tggtttcatc cgcgttgaca
     5641 aacagctgcg taccaacgta ccgcacatct ttgctatcgg cgatatcgtc ggtcaaccga
     5701 tgctggcaca caaaggtgtt cacgaaggtc acgttgccgc tgaagttatc gccggtaaga
     5761 aacactactt cgatccgaaa gttatcccgt ccatcgccta taccgaacca gaagttgcat
     5821 gggtgggtct gactgagaaa gaagcgaaag agaaaggcat cagctatgaa accgccacct
     5881 tcccgtgggc tgcttctggt cgtgctatcg cttccgactg cgcagacggt atgaccaagc
     5941 tgattttcga caaagaatct caccgtgtga tcggtggtgc gattgtcggt actaacggcg
     6001 gcgagctgct gggtgaaatc ggcctggcaa tcgaaatggg ttgtgatgct gaagacatcg
     6061 cactgaccat ccacgcgcac ccgactctgc acgagtctgt gggcctggcg gcagaagtgt
     6121 tcgaaggtag cattaccgac ctgccgaacc cgaaagcgaa gaagaagtaa tggtacccgg
     6181 ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag
     6241 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
     6301 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     6361 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
     6421 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     6481 gaacgctctc ctgagtagga caaatccgtc gggagcggat ttgaacgttg cgaagcaacg
     6541 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
     6601 ggccatcctg acggatggcc ttttactagt gcctggggtg cctaatgagt gagctaactc
     6661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6721 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg catgcataaa
     6781 aactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     6841 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     6901 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     6961 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     7021 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     7081 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     7141 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
     7201 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     7261 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     7321 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     7381 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     7441 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     7501 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
     7561 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
     7621 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     7681 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc gcccgcgttc ctgctggcgc
     7741 tgggcctgtt tctggcgctg gacttcccgc tgttccgtca gcagcttttc gcccacggcc
     7801 ttgatgatcg cggcggcctt ggcctgcata tcccgattca acggccccag ggcgtccaga
     7861 acgggcttca ggcgctcccg aaggtctcgg gccgtctctt gggcttgatc ggccttcttg
     7921 cgcatctcac gcgctcctgc ggcggcctgt agggcaggct catacccctg ccgaaccgct
     7981 tttgtcagcc ggtcggccac ggcttccggc gtctcaacgc gctttgagat tcccagcttt
     8041 tcggccaatc cctgcggtgc ataggcgcgt ggctcgaccg cttgcgggct gatggtgacg
     8101 tggcccactg gtggccgctc cagggcctcg tagaacgcct gaatgcgcgt gtgacgtgcc
     8161 ttgctgccct cgatgccccg ttgcagccct agatcggcca cagcggccgc aaacgtggtc
     8221 tggtcgcggg tcatctgcgc tttgttgccg atgaactcct tggccgacag cctgccgtcc
     8281 tgcgtcagcg gcaccacgaa cgcggtcatg tgcgggctgg tttcgtcacg gtggatgctg
     8341 gccgtcacga tgcgatccgc cccgtacttg tccgccagcc acttgtgcgc cttctcgaag
     8401 aacgccgcct gctgttcttg gctggccgac ttccaccatt ccgggctggc cgtcatgacg
     8461 tactcgaccg ccaacacagc gtccttgcgc cgcttctctg gcagcaactc gcgcagtcgg
     8521 cccatcgctt catcggtgct gctggccgcc cagtgctcgt tctctggcgt cctgctggcg
     8581 tcagcgttgg gcgtctcgcg ctcgcggtag gcgtgcttga gactggccgc cacgttgccc
     8641 attttcgcca gcttcttgca tcgcatgatc gcgtatgccg ccatgcctgc ccctcccttt
     8701 tggtgtccaa ccggctcgac gggggcagcg caaggcggtg cctccggcgg gccactcaat
     8761 gcttgagtat actcactaga ctttgcttcg caaagtcgtg accgcctacg gcggctgcgg
     8821 cgccctacgg gcttgctctc cgggcttcgc cctgcgcggt cgctgcgctc ccttgccagc
     8881 ccgtggatat gtggacgatg gccgcgagcg gccaccggct ggctcgcttc gctcggcccg
     8941 tggacaaccc tgctggacaa gctgatggac aggctgcgcc tgcccacgag cttgaccaca
     9001 gggattgccc accggctacc cagccttcga ccacataccc accggctcca actgcgcggc
     9061 ctgcggcctt gccccatcaa tttttttaat tttctctggg gaaaagcctc cggcctgcgg
     9121 cctgcgcgct tcgcttgccg gttggacacc aagtggaagg cgggtcaagg ctcgcgcagc
     9181 gaccgcgcag cggcttggcc ttgacgcgcc tggaacgacc caagcctatg cgagtggggg
     9241 cagtcgaagg gcgaagcccg cccgcctgcc ccccgagcct cacggcggcg agtgcggggg
     9301 ttccaagggg gcagcgccac cttgggcaag gccgaaggcc gcgcagtcga tcaacaagcc
     9361 ccggaggggc cactttttgc cggaggggga gccgcgccga aggcgtgggg gaaccccgca
     9421 ggggtgccct tctttgggca ccaaagaact agatataggg cgaaatgcga aagacttaaa
     9481 aatcaacaac ttaaaaaagg ggggtacgca acagctcatt gcggcacccc ccgcaatagc
     9541 tcattgcgta ggttaaagaa aatctgtaat tgactgccac ttttacgcaa cgcataattg
     9601 ttgtcgcgct gccgaaaagt tgcagctgat tgcgcatggt gccgcaaccg tgcggcaccc
     9661 ctaccgcatg gagataagca tggccacgca gtccagagaa atcggcattc aagccaagaa
     9721 caagcccggt cactgggtgc aaacggaacg caaagcgcat gaggcgtggg ccgggcttat
     9781 tgcgaggaaa cccacggcgg caatgctgct gcatcacctc gtggcgcaga tgggccacca
     9841 gaacgccgtg gtggtcagcc agaagacact ttccaagctc atcggacgtt ctttgcggac
     9901 ggtccaatac gcagtcaagg acttggtggc cgagcgctgg atctccgtcg tgaagctcaa
     9961 cggccccggc accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg gccagccccg
    10021 cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg acgaccagga
    10081 cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg accctgtatc cgggcgagca
    10141 gcaactaccg accggccccg gcgaggagcc gcccagccag cccggcattc cgggcatgga
    10201 accagacctg ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct
    10261 gccgatgccc gatgagccgt gttttctgga cgatggcgag ccgttggagc cgccgacacg
    10321 ggtcacgctg ccgcgccggt agcacttggg ttgcgcagca acccgtaagt gcgctgttcc
    10381 agactatcgg ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
    10441 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg
    10501 attcaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac
    10561 ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact
    10621 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
    10681 ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta
    10741 ttatcactta ttcaggcgta gca
//
