LOCUS       62056_3685        6038 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1792..1810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2734..2750
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2784..2800)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2833..2851)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2861..2877)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3587..4246)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(4247..5016)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtaccat gactgatttg ccggataccg acttcactca
     1861 acgcttcatc ttcgatgaga gcgacacccg cggcgagctg gtggcgctgg agcgcagtta
     1921 cgcggaagtc ctcgccaaac acccctatcc cgagccggtt gcacagctgc ttggcgaact
     1981 gatggcggcg gcatcgttgc tggtcggcac cctgaaattc gatggcttgc tgattctcca
     2041 ggcccgctcc gaaggcccgg tgccgctgct gatgatcgag tgctccagcg agcgcgagat
     2101 ccgtggcctg gcccgttacg acgcggagca gatcgcccct gatgccaccc tcgccgacct
     2161 gatgcccaac ggcgtcctgg ccctgaccgt ggacccgacc aatggccagc gctaccaggg
     2221 catcgtcgac ctggacggca ccaacctggc ggaatgcttc accaactact tcgtcatgtc
     2281 gcaacagacc ggcacccgtt tctggctcta tgccgacggc cgcagcgccc gtggcctgct
     2341 cctgcaacaa ctgcccgccg accgcctgcg tgatcaggaa gagcgtgacg ccagctggca
     2401 gcacctcact gccctggcca gcaccctgac cgccgatgag ctgctgagcc tggacaacga
     2461 aaccgtgctc caccgcctgt accacgaaga agccgtgcgc ctgttcgacg tgcagccgct
     2521 gcgttttcgc tgcagttgct cgcgggaacg gtcgggcaat gcgctggtca gcctgggtct
     2581 ggaagatgcc cagcaactgg tggtcgaaca tggcggcagc atcgagatcg attgccagtt
     2641 ctgcaacgaa cgctacctgt tcgatgccgc tgatatcgcg caattgttcg cgggggcggg
     2701 cgtggacacg ccgtcagata ctcgtcacta actcgaggtc gacggtatcg ataagcttga
     2761 tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga
     2821 gctccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     2881 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2941 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3001 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3061 ttgttaaatc agctcatttt ttaaccaata ggccgactgc gatgagtggc agggcggggc
     3121 gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggtgctacgc ctgaataagt
     3181 gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc gtcggttcag
     3241 ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg
     3301 gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg
     3361 tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg ataaaaacgg
     3421 tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
     3481 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc tacagccgat
     3541 agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg cgcggcagcg
     3601 tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc tcatcgggca
     3661 tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag gctggcaggt
     3721 ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg ccggtcggta
     3781 gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc cccaacagcg
     3841 attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac aggcgcaact
     3901 ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac acggtgccgg
     3961 ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg actgcgtatt
     4021 ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg accaccacgg
     4081 cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc gtgggtttcc
     4141 tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc cagtgaccgg
     4201 gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt atctccatgc
     4261 ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg
     4321 acaacaatta tgcgttgcgt aaaagtggca gtcaattaca gattttcttt aacctacgca
     4381 atgagctatt gcggggggtg ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt
     4441 gatttttaag tctttcgcat ttcgccctat atctagttct ttggtgccca aagaagggca
     4501 cccctgcggg gttcccccac gccttcggcg cggctccccc tccggcaaaa agtggcccct
     4561 ccggggcttg ttgatcgact gcgcggcctt cggccttgcc caaggtggcg ctgccccctt
     4621 ggaacccccg cactcgccgc cgtgaggctc ggggggcagg cgggcgggct tcgccttcga
     4681 ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag ccgctgcgcg
     4741 gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag cgaagcgcgc
     4801 aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg ggcaaggccg
     4861 caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc ggtgggcaat
     4921 ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca gcagggttgt
     4981 ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc cacatatcca
     5041 cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca agcccgtagg
     5101 gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca aagtctagtg agtatactca
     5161 agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc cccgtcgagc cggttggaca
     5221 ccaaaaggga ggggcaggca tggcggcata cgcgatcatg cgatgcaaga agctggcgaa
     5281 aatgggcaac gtggcggcca gtctcaagca cgcctaccgc gagcgcgaga cgcccaacgc
     5341 tgacgccagc aggacgccag agaacgagca ctgggcggcc agcagcaccg atgaagcgat
     5401 gggccgactg cgcgagttgc tgccagagaa gcggcgcaag gacgctgtgt tggcggtcga
     5461 gtacgtcatg acggccagcc cggaatggtg gaagtcggcc agccaagaac agcaggcggc
     5521 gttcttcgag aaggcgcaca agtggctggc ggacaagtac ggggcggatc gcatcgtgac
     5581 ggccagcatc caccgtgacg aaaccagccc gcacatgacc gcgttcgtgg tgccgctgac
     5641 gcaggacggc aggctgtcgg ccaaggagtt catcggcaac aaagcgcaga tgacccgcga
     5701 ccagaccacg tttgcggccg ctgtggccga tctagggctg caacggggca tcgagggcag
     5761 caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc ctggagcggc caccagtggg
     5821 ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc tatgcaccgc agggattggc
     5881 cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa gccgtggccg accggctgac
     5941 aaaagcggtt cggcaggggt atgagcctgc cctacaggcc gccgcaggag cgcgtgagat
     6001 gcgcaagaag gccgatcaag cccaagagac ggcccgag
//
