LOCUS       V009263                 6252 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009263
VERSION     V009263
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 6252)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6252)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             1793..2452
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     promoter        3171..3199
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3207..3223
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3245..3262
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3263..4375
                     /gene="rppA"
                     /label="1,3,6,8-tetrahydroxynaphthalene synthase"
                     /note="1,3,6,8-tetrahydroxynaphthalene synthase from
                     Streptomyces griseus. Accession#: Q54240"
     regulatory      4422..4568
                     /label="rrnBT1T2"
                     /note="rrnBT1T2"
                     /regulatory_class="terminator"
     protein_bind    complement(4575..4596)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5004..5795)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttgact
     3001 gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc
     3061 aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt
     3121 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta
     3181 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3241 aatgcaccat caccatcacc atgcgaccct gtgccgaccg gccatcgctg tgcccgagca
     3301 cgtcatcacg atgcagcaga ccctggacct ggcccgggag acccatgccg ggcacccgca
     3361 gcgcgacctc gtcctgaggc tcatccagaa caccggcgtc cagacccggc acctcgtgca
     3421 gcccatcgag aagaccctgg cgcaccccgg attcgaggtg cgcaaccagg tgtacgaggc
     3481 cgaggccaag acccgggtcc ccgaagtcgt ccggcgggcg ctcgccaacg ccgagaccga
     3541 gccgtccgag atcgacctga tcgtctacgt ctcctgcacg ggtttcatga tgccctcgct
     3601 gaccgcgtgg atcatcaaca gcatgggctt ccggcccgag acccgccaac tgcccatcgc
     3661 ccagctcggc tgtgcggcgg gcggcgcggc gatcaaccgc gcgcacgact tctgcgtggc
     3721 ctaccccgac tccaacgtcc tcatcgtgtc ctgcgagttc tgctcgctgt gctaccagcc
     3781 caccgacatc ggggtcggtt ccctgctctc caacggactc ttcggcgacg cgctctccgc
     3841 ggccgtcgta cggggacagg gcggcaccgg catgcgcctg gagcgcaacg gctcccacct
     3901 ggtgcccgac accgaggact ggatctccta cgcggtccgc gacaccgggt tccacttcca
     3961 gctggacaag cgggtcccgg gcaccatgga gatgctcgcc ccggtgctcc tggacctggt
     4021 cgacctgcac ggctggtccg tcccgaacat ggacttcttc atcgtccacg cgggcggacc
     4081 gcgcatcctg gacgacctct gccacttcct cgacctgccg cccgagatgt tccgctacag
     4141 ccgggccacc ctcaccgaac gcggcaacat cgcgagctcc gtcgtcttcg acgcgctggc
     4201 gcgcctcttc gacgacggcg gcgccgccga gtccgcgcag gggctcatcg ccggcttcgg
     4261 tcccggcatc accgccgagg tggccgtggg gagttgggcc aaggaaggcc tcggggcgga
     4321 cgtcggacgc gacctcgacg agctggagct gaccgccggc gttgcgctgt ccggctaatg
     4381 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggctgt tttggcggat
     4441 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     4501 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     4561 tgaaacgctg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4621 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4681 ggcggtttgc gtattgggcg catgcataaa aactgttgta attcattaag cattctgccg
     4741 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     4801 tcgccttgcg tataatattt gcccatgggg gtgggcgaag aactccagca tgagatcccc
     4861 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     4921 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     4981 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     5041 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     5101 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     5161 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     5221 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
     5281 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     5341 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     5401 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     5461 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     5521 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     5581 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
     5641 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     5701 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     5761 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     5821 tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     5881 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt
     5941 gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc
     6001 tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccca
     6061 ggtggcactt ttcggggaaa tgtgcgcgcc cgcgttcctg ctggcgctgg gcctgtttct
     6121 ggcgctggac ttcccgctgt tccgtcagca gcttttcgcc cacggccttg atgatcgcgg
     6181 cggccttggc ctgcatatcc cgattcaacg gccccagggc gtccagaacg ggcttcaggc
     6241 gctcccgaag gt
//
