LOCUS       40924_5999        6640 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBBR1-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Ouahrani-Bettache S, Porte F, Teyssier J, Liautard JP, Kohler S.
  TITLE     pBBR1-GFP: a broad-host-range vector for prokaryotic promoter 
            studies
  JOURNAL   BioTechniques 26 (4), 620-622 (1999)
  PUBMED    10343896
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Ouahrani-Bettache S, Porte F, Teyssier J, Liautard JP, Koehler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-1998) INSERM U-431, University of Montpellier II, 
            Pl. E. Bataillon, Montpellier 34095, France
REFERENCE   3  (bases 1 to 6640)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6640)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1999"; volume: "26"; issue: "4"; pages: 
            "620-622"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-1998) INSERM U-431, University of Montpellier II, Pl. E. 
            Bataillon, Montpellier 34095, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2614..2630
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2664..2680)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3110..3130)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(3260..3325)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(3494..4150)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    complement(4183..4204)
                     /label=from pBBR1MCS: GenBank Accession Number U02374
                     /note="from pBBR1MCS: GenBank Accession Number U02374"
     CDS             complement(4216..4929)
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    4983..4997
                     /label=from pBBR1MCS: GenBank Accession number U02374
                     /note="from pBBR1MCS: GenBank Accession number U02374"
     CDS             complement(5572..6429)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    6447..6640
                     /label=from pBBR1MCS: GenBank Accession Number U02374
                     /note="from pBBR1MCS: GenBank Accession Number U02374"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg
     2641 caggaattcg atatcaagct tatcgatacc gtcgacctcg aggtcaacaa caacaattgc
     2701 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2761 ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt
     2821 ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat
     2881 taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac
     2941 tgttatgcct acttataaag gttacagaat atttttccat aattttcttg tatagcagtg
     3001 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
     3061 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
     3121 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
     3181 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
     3241 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
     3301 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
     3361 attatgtcac accacagaag taaggttcct tcacaaagat ccggcgaatt tctgccattc
     3421 atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc
     3481 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     3541 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
     3601 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     3661 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     3721 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     3781 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     3841 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     3901 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     3961 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     4021 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     4081 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     4141 ttttctccat tttagcttcc ttagctcctg aaaatctcgc caagcttgat atcgaattcc
     4201 tgcaggtctg gactatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
     4261 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
     4321 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
     4381 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
     4441 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
     4501 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
     4561 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
     4621 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
     4681 aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac cataaccgaa
     4741 agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag
     4801 ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtgccc
     4861 attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc
     4921 tttactcata tgtatatctc cttcttaaat ctagatttaa agatctggta ccgggccccc
     4981 cctcgagggg gggcccgttt ctcaaagctc acgctgtagg tatctcagtt cggtgtaggt
     5041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5101 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5161 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5221 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5281 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5341 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5401 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5461 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5521 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5581 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5641 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5701 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5761 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5821 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5881 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5941 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6001 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6061 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6121 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6181 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6241 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6301 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6361 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6421 aatactcata ctcttccttt ttcaatcagg tggcactttt cggggaaatg tgcgcgcccg
     6481 cgttcctgct ggcgctgggc ctgtttctgg cgctggactt cccgctgttc cgtcagcagc
     6541 ttttcgccca cggccttgat gatcgcggcg gccttggcct gcatatcccg attcaacggc
     6601 cccagggcgt ccagaacggg cttcaggcgc tcccgaaggt
//
