LOCUS       40924_5974        6702 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBBR-IBA3plus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     MipZ, a Spatial Regulator Coordinating Chromosome Segregation with 
            Cell Division in Caulobacter
  JOURNAL   Cell 126 (1), 147-162 (2006)
  PUBMED    16839883
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2005) Developmental Biology, Stanford University, 
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 6702)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6702)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2006"; volume: "126"; issue: "1"; pages: "147-162"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-OCT-2005) Developmental Biology, Stanford University, 279 Campus
            Drive, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(264..794)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(983..1011)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1201..1256
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             1264..1287
                     /codon_start=1
                     /gene="tetA'"
                     /product="TetA'"
                     /label=tetA'
                     /note="TetA-derived mini ORF"
                     /protein_id="ABB71439.1"
                     /translation="MNSSTKI"
     gene            1264..1287
                     /gene="tetA'"
                     /label=tetA'
     RBS             1295..1317
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1409..1432
                     /codon_start=1
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
                     /translation="WSHPQFEK"
     rep_origin      1523..1978
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2005..2109
                     /label=AmpR promoter
     CDS             2110..2967
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             2977..3600
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline repressor TetR"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             complement(4251..4910)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(4911..5680)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
      241 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
      301 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
      361 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
      421 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
      481 tcactacgcg gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct
      541 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
      601 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
      661 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
      721 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      781 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      841 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      901 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
      961 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1021 tgccttcatc cgtttccacg gtgtgcgtcc atgggcaaat attatacgca aggcgacaag
     1081 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     1141 agaatgctta atgaattaca acagttttta tgatggccag atgattaatt cctaattttt
     1201 gttgacactc tatcattgat agagttattt taccactccc tatcagtgat agagaaaagt
     1261 gaaatgaata gttcgacaaa aatctagaaa taattttgtt taactttaag aaggagatat
     1321 acaaatggga gaccgcggtc ccgaattcga gctcggtacc cggggatccc tcgaggtcga
     1381 cctgcagggg gaccatggtc tcagcgcttg gagccacccg cagttcgaaa aataataagc
     1441 ttgacctgtg aagtgaaaaa tggcgcacat tgtgcgacat tttttttgtc tgccgtttac
     1501 cgctactgcg tcacggatct ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1681 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1861 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1921 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttca
     1981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     2341 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     2401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2461 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2521 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2641 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2701 actctagctt cccggcaaca attgatagac tggatggagg cggataaagt tgcaggacca
     2761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2821 cgtggctctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2881 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2941 ataggtgcct cactgattaa gcattggtag gaattaatga tgtctcgttt agataaaagt
     3001 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     3061 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     3121 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     3181 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     3241 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     3301 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     3361 gagaatgcat tatatgcact cagcgcagtg gggcatttta ctttaggttg cgtattggaa
     3421 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     3481 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     3541 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct
     3601 taaaagcagc ataacctttt tccgtgatgg taacttcact agcgcccttc ccaacagttg
     3661 cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3721 atttttgtta aatcagctca ttttttaacc aataggccga ctgcgatgag tggcagggcg
     3781 gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggtgct acgcctgaat
     3841 aagtgataat aagcggatga atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt
     3901 tcagggcagg gtcgttaaat agccgcttat gtctattgct ggtttaccgg tttattgact
     3961 accggaagca gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc tcaggctctc
     4021 cccgtggagg taataattga cgatatgatc atttattctg cctcccagag cctgataaaa
     4081 acggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
     4141 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgagg cggctacagc
     4201 cgatagtctg gaacagcgca cttacgggtt gctgcgcaac ccaagtgcta ccggcgcggc
     4261 agcgtgaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg
     4321 ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc
     4381 aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc
     4441 ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac
     4501 agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc
     4561 aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc cgacacggtg
     4621 ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg
     4681 tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc
     4741 acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt
     4801 ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg cacccagtga
     4861 ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat gcttatctcc
     4921 atgcggtagg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac ttttcggcag
     4981 cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta
     5041 cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt
     5101 tgttgatttt taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag
     5161 ggcacccctg cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc
     5221 ccctccgggg cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc
     5281 ccttggaacc cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgcct
     5341 tcgactgccc ccactcgcat aggcttgggt cgttccaggc gcgtcaaggc caagccgctg
     5401 cgcggtcgct gcgcgagcct tgacccgcct tccacttggt gtccaaccgg caagcgaagc
     5461 gcgcaggccg caggccggag gcttttcccc agagaaaatt aaaaaaattg atggggcaag
     5521 gccgcaggcc gcgcagttgg agccggtggg tatgtggtcg aaggctgggt agccggtggg
     5581 caatccctgt ggtcaagctc gtgggcaggc gcagcctgtc catcagcttg tccagcaggg
     5641 ttgtccacgg gccgagcgaa gcgagccagc cggtggccgc tcgcggccat cgtccacata
     5701 tccacgggct ggcaagggag cgcagcgacc gcgcagggcg aagcccggag agcaagcccg
     5761 tagggcgccg cagccgccgt aggcggtcac gactttgcga agcaaagtct agtgagtata
     5821 ctcaagcatt gagtggcccg ccggaggcac cgccttgcgc tgcccccgtc gagccggttg
     5881 gacaccaaaa gggaggggca ggcatggcgg catacgcgat catgcgatgc aagaagctgg
     5941 cgaaaatggg caacgtggcg gccagtctca agcacgccta ccgcgagcgc gagacgccca
     6001 acgctgacgc cagcaggacg ccagagaacg agcactgggc ggccagcagc accgatgaag
     6061 cgatgggccg actgcgcgag ttgctgccag agaagcggcg caaggacgct gtgttggcgg
     6121 tcgagtacgt catgacggcc agcccggaat ggtggaagtc ggccagccaa gaacagcagg
     6181 cggcgttctt cgagaaggcg cacaagtggc tggcggacaa gtacggggcg gatcgcatcg
     6241 tgacggccag catccaccgt gacgaaacca gcccgcacat gaccgcgttc gtggtgccgc
     6301 tgacgcagga cggcaggctg tcggccaagg agttcatcgg caacaaagcg cagatgaccc
     6361 gcgaccagac cacgtttgcg gccgctgtgg ccgatctagg gctgcaacgg ggcatcgagg
     6421 gcagcaaggc acgtcacacg cgcattcagg cgttctacga ggccctggag cggccaccag
     6481 tgggccacgt caccatcagc ccgcaagcgg tcgagccacg cgcctatgca ccgcagggat
     6541 tggccgaaaa gctgggaatc tcaaagcgcg ttgagacgcc ggaagccgtg gccgaccggc
     6601 tgacaaaagc ggttcggcag gggtatgagc ctgccctaca ggccgccgca ggagcgcgtg
     6661 agatgcgcaa gaaggccgat caagcccaag agacggcccg ag
//
