LOCUS       40924_5954        3778 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBB-CmR-ccdB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Fulwiler AL, Soysa DR, Ullman B, Yates PA.
  TITLE     A rapid, efficient and economical method for generating leishmanial 
            gene targeting constructs
  JOURNAL   Mol. Biochem. Parasitol. 175 (2), 209-212 (2011)
  PUBMED    21055426
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Yates PA, Fulwiler AL, Soysa DR, Ullman B.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3778)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3778)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Biochem. Parasitol."; date: "2011"; volume: "175"; issue: "2"; 
            pages: "209-212"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2010) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..13
                     /label=SfiI restriction site
                     /note="SfiI restriction site"
     promoter        195..299
                     /label=AmpR promoter
     CDS             300..1157
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1331..1919
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2189..2201
                     /label=SfiI restriction site
                     /note="SfiI restriction site"
     CDS             complement(2211..2513)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(2858..3514)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3515..3617)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ggccagtcag gccttaatta agtttaaact ggaacaacac tcaaccctat ctcggtctat
       61 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      121 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
      181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1441 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     1501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2101 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2161 acgacaggtt tcgtttaaac ttaattaagg ccacctaggc ctgacattta tattccccag
     2221 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
     2281 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
     2341 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca
     2401 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc
     2461 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
     2521 gtccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat
     2581 cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat
     2641 ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg
     2701 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac acctcttttt gacatacttc
     2761 gggtatacat atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact
     2821 gttatctggc ttttagtaag ccggatccac gcgattacgc cccgccctgc cactcatcgc
     2881 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat
     2941 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     3001 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     3061 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     3121 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     3181 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     3241 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt
     3301 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     3361 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     3421 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     3481 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     3541 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     3601 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
     3661 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt
     3721 cggcgcaaag tgcgtcgggt gatgctgcca acttagtcga cgctctagag ctccaaat
//
