LOCUS       V009273                 8946 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009273
VERSION     V009273
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8946)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8946)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88186.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     promoter        1779..1797
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(1876..2265)
                     /label="3' UTR of mCdh1"
                     /note="3' UTR of mCdh1"
     CDS             complement(2269..4920)
                     /gene="Cdh1"
                     /label="Cadherin-1"
                     /note="Cadherin-1 from Mus musculus. Accession#: P09803"
     promoter        complement(4996..5014)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(5081..5226)
                     /label="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     misc_feature    complement(5342..6336)
                     /label="5' UTR of chicken beta-actin"
                     /note="5' UTR of chicken beta-actin"
     promoter        complement(6337..6612)
                     /label="chicken beta-actin promoter"
     rep_origin      complement(7119..7707)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7881..8738)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8739..8843)
                     /label="AmpR promoter"
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgggaccaaa gcggccatcg
     1621 tgcctcccca ctcctgcagt tcgggggcat ggatgcgcgg atagccgctg ctggtttcct
     1681 ggatgccgac ggatttgcac tgccggtaga actccgcgag gtcgtccagc ctcaggcagc
     1741 agctgaacca actcgcgagg ggatcgagcc catcaaatta atacgactca ctatagggag
     1801 aagcttgcat gccacgcgtc tcgagggccc ctgcaggtcg actctagagg atccccgggt
     1861 accgagctcg aagaattcag gaacatgagt gactattaga atacagcaaa catggtaatt
     1921 gagggcaagt acacaatgta aaaaattagt gaggtgtgag gggcttgggg ctgttgtgtc
     1981 tggggcagat catagctcaa aagaacaaaa acagaaacaa acaaacaaaa aaaggcacag
     2041 tttatatctc agcactgtaa aaataaaaaa taaaaaaaga tttcaacaat cagtgaaaac
     2101 catcctaaca cagacagtcc agtagggaga gtgagaccac tatgcaatct gcacacttct
     2161 cagatcagtc tctcttctac acactcaggg aaggagctgg aaagaccact gatataatta
     2221 ttctgcatct cccatggtgc cacacggggg agacttgcta gtcccctagt cgtcctcgcc
     2281 accgccgtac atgtccgcca gcttcttgaa tcggttgccc cactcgttca gataatcgta
     2341 gtcctggtcc tgatccgact cagaggagtt cagtgagctc aggctagcgg cttcagaacc
     2401 actgccctcg taatcgaaca ccaacagaga gtcgtaaggg ggtgccgtgg ggtcgctgtc
     2461 ggctgccttc aggttttcat cgatgaagtt tccaatttca tcaggattgg caggacgggg
     2521 acgatactgg ggcacgctca tgagggtggg agccacatca tttcgagtca cttccggtcg
     2581 ggcatccagg cccctgtgca gctggctcaa atcaaagtcc tggtcttctt ctccacctcc
     2641 ttcttcatca tagtaataca cattgtcccg ggtatcatca tctggtggca gcaggggctc
     2701 tttgaccacc gttctcctcc gtagaaacag taggagcagc aggatcagaa tcagcagggc
     2761 gaggatccct ccaaggattc cgaggatggc aggaacttgc aatcctgctg ccacgattcc
     2821 cgccttcatg cagttgttga ccgtcccttc acagtcacac acatggacgt ccaacgtggt
     2881 cacctggtct ttgttctggt tatccgcgag cttgagatgg attttgtatt cgccaatctc
     2941 taagtccttt cttggttgca aaatgagaga ttcttgagct gcgtcattat actcaatggt
     3001 ccagttgacg ctggccccat gggttagctc agcagtaaag ggggacgtgt tggggggaag
     3061 gtctggatcc aagatggtga tgatatgagg ctgtgggttc ctctggcaga actgcatgtt
     3121 tcgaggttct gggatgggag cgttgtcatt gacgtctaac aggaccagga gaagagtgcc
     3181 cgtgccagtg gcaatgggtg aaccatcatc tgtggcgatg atgagagcta catatgtgct
     3241 gttcttcaca tgctcagcgt cttctctgtc catctcagcg cgcgtgaaaa tggcaccagt
     3301 ctctgggtta atctccagcc agttggcagt gtccctccaa atccgatacg tgatcttctg
     3361 atccatgaac gtgtccggct ctcgagcggt ataagatgtg atttcctgac ccacaccaaa
     3421 gtcttcgggc acttcgactc tcctctccgc aggcataaag atgggggctt cattcacgtc
     3481 taccacgtcc acagtgacag tggctgtgga agggacaaga gacccctcaa agggttcctc
     3541 gttctccact ctcacatgaa ggatgtattg ctgcttggcc tcaaaatcca agcccttggc
     3601 tgttttcaaa atgccatcat tggtcgtggg gtctgtgacg acaacgaact gctggtcagg
     3661 atcgttgact acggtgtaca cagctttcca cgccggagtg ttgggggcat catcatcggt
     3721 cactttgagt gtggcgatcc gggcattgac ctcattctca ggcacttgac cctgatacgt
     3781 gctcgggttg aagacaggag cgttgtcatt aatatccttg acagtgatca cagccttggc
     3841 tgttgtgctc aagccttcgc cttgaaggtc agcagcctga accaccagag tgtatgtagg
     3901 gtaactctct cggtccagcc cagaggtgag cacactgatg accccggtgt ccctattgac
     3961 agtgaacatg tttttgtgag gcagctcagg atcctggctg acgatggtgt aggcgatggc
     4021 agcgttgtag gtgttgacgt catcgtctgc atcggtggct gagaccttca tcacggaggt
     4081 tcctggaaca gcgccttctg caacggatcc ctcaaacacc tcctgggtaa actctggcct
     4141 gttgtcattc tgatctgtca ctgtgatcac tatctccatg ggatcctcca ccgcttcccc
     4201 atttgatgac acggcatgag aatagaggat gtacttggca atggcttctc tatccagagg
     4261 ctgtgtcact ttcagccagc ctgtctccct ctcaatgatg aaaacgccaa cggggggttt
     4321 gtcagctcct tggccggtga tgctgtagaa aacctttgtt tctttgtccc tgttggattt
     4381 gatctgaacc aggttctttg ggaattcacc cttttcattt tcggggcagc tgatgggagg
     4441 gatgacccag tctcgtttct gtcttctgag acctgggtac acgctgggaa acatgagcag
     4501 ctctgggttg gattcagagg cagggtcgcg gtggtggtgc cggtgatggt ggtgccccat
     4561 ggacttcagc gtcactttgg tagacagctc cctatgactg gagtcccggg cgcggacgag
     4621 gaaactggtc tccagcttgt ggagctttag atgccgcttc actgtgatgg tgccgtctgt
     4681 cgccactttg aatcgggagt cttccgaaaa gaaggctgtc cttggccggc cggtgcatcc
     4741 ttcaaatctc actctgccca ggacatggcc tctctccagg tgcctctccg gcaccgggaa
     4801 ggtgtagacc tcggaactga agccgggact gcaggactca ggctccagct cctggcaaag
     4861 ccatgaggag acctgcagca ggagcaggag cgcggaaaag ctgcggcacc gggctcccat
     4921 ggcggacccg gcagacgccg agcaaacact gagctcgggt gcggtcgggc agggccgggt
     4981 taggccggcc cgaattctat agtgtcacct aaatcgtatg tgtatgatac aaaaggttat
     5041 gtattaattg taagggatct gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg
     5101 tcgctcggtg ttcgaggcca cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg
     5161 ccgccccgac tgcatctgcg tgttcgaatt cgccaatgac aagacgctgg gcggggtttg
     5221 tgtcatcata gaactaaaga catgcaaata tatttcttcc ggggacaccg ccagcaaacg
     5281 cgagcaacgg gccacgggga tgaagcagaa gcttnnnnna gatcctctag agtcgacngc
     5341 tggctgcgga ggaacagaga agggaagata taaaccccgc cggtcacacg ccagaagccg
     5401 aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cctgcggcag ccccgaggct
     5461 ggagatggag aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct
     5521 tcctgccggc gccgcaccgc ttcgcccgcg cccgctagag ggggtgcggc ggcgcctccc
     5581 agatttcggc tccgccagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac
     5641 cataaaaggc aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccggggccgc
     5701 cgcgcccctc ccccgagccc tccccggccc gaggcggccc cgccccgccc ggcaccccca
     5761 cctgccgcca ccccccgccc ggcacggcga gccccgcgcc acgccccgca cggagccccg
     5821 cacccgaagc cgggccgtgc tcagcaactc ggggaggggg gtgcaggggg gggttacagc
     5881 ccgaccgccg cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct
     5941 ttgttcccct cgcagccccc ccgcaccgcg gggcaccgcc cccggccgcg ctcccctcgc
     6001 gcacacgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag
     6061 cgcgggccgc acgcggcgct ccccacgcac acacacacgc acgcaccccc cgagccgctc
     6121 ccccccgcac aaagggccct cccggagccc tttaaggctt tcacgcagcc acagaaaaga
     6181 aacgagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc
     6241 gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcgcg
     6301 gagcggggca cggggcgaag gcaacgcagc gactcccgcc cgccgcgcgc ttcgcttttt
     6361 atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg
     6421 attggctgcc gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc
     6481 gcctggcgcg cgcccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa
     6541 ataaatacaa aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg
     6601 ggctcacctc gaggtgaggg tgtggggcgg gggtgtgacg cgcccctcca tcccccctcc
     6661 cccctttttg attccacagt gctttgttgg ggagggggag cggggtccgc gtgtgcagcc
     6721 ctccccccac ccccgaggtg gtcctgcggt ccccttgaag tcacctcccg gggagcgcca
     6781 gcaggagagg cagggatagc gatgggcaat gagcgtgcat gcactgagcn nnactggctt
     6841 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     6901 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     6961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7681 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7861 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7921 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7981 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8041 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8101 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8161 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     8221 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8281 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8341 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8401 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8461 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     8521 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8581 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8641 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8701 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8761 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8821 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8881 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     8941 caagaa
//
