LOCUS       V009274                 9691 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009274
VERSION     V009274
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Kooij TW, Rauch MM, Matuschewski K.
  TITLE     Expansion of experimental genetics approaches for Plasmodium berghei
            with versatile transfection vectors
  JOURNAL   Mol. Biochem. Parasitol. 185 (1), 19-26 (2012)
   PUBMED   22705315
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Kooij TWA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2012) Parasitology Unit, Max Planck Institute for
            Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE   3  (bases 1 to 9691)
  AUTHORS   Kooij TWA.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2012) Parasitology Unit, Max Planck Institute for
            Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE   4  (bases 1 to 9691)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Biochem. Parasitol."; date: "2012"; volume: "185"; issue: "1";
            pages: "19-26"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAY-2012) Parasitology Unit, Max Planck Institute for Infection
            Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (29-AUG-2012) Parasitology Unit, Max Planck Institute for Infection
            Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany"
            SGRef: number: 4; type: "Journal Article"
            On Aug 29, 2012 this sequence version replaced JX099571.1.
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        19..37
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    79..132
                     /note="5'MCS; multiple cloning site"
     CDS             166..870
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    904..933
                     /label="c-Myc epitope tag"
                     /note="c-Myc epitope tag"
     CDS             904..933
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    940..969
                     /label="c-Myc epitope tag"
                     /note="c-Myc epitope tag"
     CDS             940..969
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    976..1005
                     /label="c-Myc epitope tag"
                     /note="c-Myc epitope tag"
     CDS             976..1005
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     3'UTR           1021..1434
                     /note="3'PbPPPK-DHPS; 3'UTR of Plasmodium berghei
                     hydroxymethylpterin pyrophosphokinase-dihydropteroate
                     synthetase"
     5'UTR           1456..2825
                     /note="5'PbHSP70; 5'UTR of Plasmodium berghei heat shock
                     protein 70"
     CDS             2849..3562
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     3'UTR           3578..4107
                     /note="3'PbDHFS-FPGS; 3'UTR of Plasmodium berghei
                     dihydrofolate synthase-folylpolyglutamate synthase"
     3'UTR           complement(4120..4575)
                     /note="3'PbDHFR-TS; 3'UTR of Plasmodium berghei
                     dihydrofolate reductase-thymidylate synthase"
     CDS             complement(4579..5223)
                     /gene="FUR1"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P18562"
     CDS             complement(5224..5694)
                     /gene="FCY1"
                     /label="Cytosine deaminase"
                     /note="Cytosine deaminase from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: Q12178"
     CDS             complement(5737..6297)
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     5'UTR           complement(6298..7241)
                     /note="5'PbDHFR-TS; 5'UTR of Plasmodium berghei
                     dihydrofolate reductase-thymidylate synthase"
     misc_feature    complement(7248..7694)
                     /note="3'PbDHFR-TS_duplicate; duplicated sequence of 3'UTR
                     of Plasmodium berghei dihydrofolate reductase-thymidylate
                     synthase"
     misc_feature    7695..7757
                     /note="3'MCS; multiple cloning site"
     promoter        complement(7797..7814)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7819..7835)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(7996..8584)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8758..9615)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9616..9687)
                     /label="AmpR promoter"
ORIGIN
        1 tgtaaaacga cggccagtta atacgactca ctatagggga cgatcggtcg tgcactgccg
       61 gcgtcgacga agtacttctc gcgagcgcgc ccgcggcggc cgtctagaga attcagatct
      121 actagtgtta acatgggatc tggatctggt ggtggtggaa ccggtgtgag caagggcgag
      181 gaggataaca tggccatcat caaggagttc atgcgcttca aggttcacat ggagggctcc
      241 gtgaacggcc acgagttcga gatcgaggga gagggagagg gaagaccata cgagggaacc
      301 cagaccgcaa agctgaaggt gactaagggt ggtccactgc ccttcgcctg ggatattctg
      361 tcccctcagt tcatgtacgg ctccaaggct tacgtgaagc accccgccga catccccgac
      421 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      481 ggcggcgtgg tcacagtgac ccaggactcc tccttgcagg acggcgagtt catctacaag
      541 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagactatg
      601 ggctgggaag cctcctccga gcggatgtac cccgaggacg gcgctctgaa gggcgagatc
      661 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
      721 aaggccaaga agcccgtgca gcttcccggc gcttacaacg tcaacatcaa gttggacatc
      781 acctcccaca acgaggacta taccatcgtg gaacagtacg aaagagctga gggtagacac
      841 tctaccggag gaatggacga gctgtataag ggccacgtgg gcctgtctgg aggatctgga
      901 ggagaacaaa aattaatttc tgaagaagat ttagctgctg aacaaaaatt aatttctgaa
      961 gaagatttag ctgctgaaca aaaattaatt tctgaagagg atctttgcgc ataagcatgc
     1021 acttattgaa gaaaaaaata tattcttata attagtaaat taattttaac tttttcggta
     1081 ttttcacctc catatatatc tcttttagta gtattgtatt aatacttata ttacttaaaa
     1141 atatagttaa tctaaaaatg aacgaattaa attatccttc acaagattaa ataaatgaga
     1201 ctatcattaa gggtaaaaaa aaataaacgc cttcaatgga taatgtatag tggatgtagt
     1261 aatacaagtt acatgcgatg ttttactatt tagtcatatc caggaataaa catacttctt
     1321 ataaaagctc ctttttcttt ttaaaattag cacatcctta tatacattat gcacatttat
     1381 gtatgaaaat tattctcaca tattgcaaag aggggaatca tccattttgt tgtgctaagc
     1441 accagctgct tcgaagtaat attttgttgg tgagcttaaa aacttttaat attgtttgtg
     1501 caaaattttt gctaagaaaa taattttatg agtcatatta gaagttcatt gaatttgtta
     1561 tataaaataa attttatatt ctgcctattt tatgtacaac aaattgcgga attgtacata
     1621 atataatatg aaaaatatat ggctttaaat tataattgcc aaataaaata gggaggggcc
     1681 gcccccaagc cctcaaaggg tgcatactat gtttataata tattattata tagcattata
     1741 ttagcattat atatatttgt tattttacaa tacattcatt ttgcgtgggg caatgcagtt
     1801 gcgctccttt tcagttcata tttttgcaaa taattgcact ttcggaaatt tgaaaaaata
     1861 taaaacccat aaaaaggtgg aatacaaaac attttttgca ttatcgaagc atataaatat
     1921 gtatatatat tatgcagact atattatgac tatccttgcc aataaaaaaa aaaatattat
     1981 ataaagggga ccccatatat aggtatatat aatagctgct ttaactaagg ggctaataaa
     2041 tggccccgta ttttgttttt tttatagcta ataataaaaa taaaaaaata aaaagtacga
     2101 actttagtcc cataaattaa gaagtatata tgcttataaa aaggataaca aataaattaa
     2161 tgataactca aataaattaa ttttcatgca aaaaaatatt taaaaaagaa tatatatatt
     2221 aagaaaaata attataaata ttatttacat atatatacca taatatatac ccccttttga
     2281 ttatatatat ataatattat atatttttct ttagagaaaa aataaatagt gttatttcct
     2341 attatatatc gtagttaatt ttaataattt acaatacact atatttctta ttttatataa
     2401 aatatattta taattaattt ttttatatta tatgtatata ttttaattgc attctccttt
     2461 tttaataaaa attttaccca attgttatag agcttgttat tattatataa taacaatata
     2521 agaatatttt ttatataaca ttaaaaaaaa ttaacatatt ttttattttt tttaaatatt
     2581 tattccaatt tatttttaac ggtcaaattg aagaaatata tagatatata tttattgctt
     2641 aaattataag tatatgtttt tttaaaaaat taaagcccaa aacatatttc tttgtattat
     2701 atacattttt gcattttaca ctattttgcc ataagcacat taaatatata tatatatgct
     2761 tgtagcaaaa cgattaaata aaataattac caacattatt atcccaataa attacaatta
     2821 caattattta aatatgggac atatgtctgt gagtaaagga gaagaacttt tcactggagt
     2881 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
     2941 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
     3001 aaaactacct gttccctggc caacacttgt cactactttc ggttatggtg ttcaatgctt
     3061 tgcgagatac ccagatcaca tgaaacagca tgactttttc aagagtgcca tgcccgaagg
     3121 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgcgctga
     3181 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
     3241 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgttta
     3301 catcatggca gacaaacaaa agaatggaat caaagtaaac ttcaaaatta gacacaacat
     3361 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
     3421 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgccctat cgaaagatcc
     3481 caacgaaaag agagaccaca tggtccttct tgagtttgta acagcagcag ggattacaca
     3541 tggcatggat gaactataca aaggcgccta aggatcctag ctaaaaggtg tgcaagcccg
     3601 tttaaggatt attaaatggc tagtaaaaga aataataata ataaaaaata ataataaaat
     3661 atattgaact atctaaatac acataataca gagcacccat gatgtaaaat aataaatggg
     3721 aatacaaatg gatagtacaa aatacattct ataataaaaa ttccttaata taaaacaatt
     3781 ttaagtcgat aaagcaagca tattatctga aaatcgaact taggatcgtt tttgtttttg
     3841 gccaattaaa gataacatca acattgatag cgatatagcg aaaagcattt agtaattaat
     3901 cgaataatat atttatttct tatttatttt atcgtttaaa agtaatttca tacaaattct
     3961 aaaaaaaata attttttatt tgaaaaatta agaacaagac atcaaaataa aaatttataa
     4021 gtatataatt aatgaaaaat aaaaattttt taaatatact ttttatgtaa caaaaaaata
     4081 tacgtgaaaa agcatcatta caaatatgat atccggagct cgaaattgaa ggaaaaaaca
     4141 tcatttgtgt ttatatgttt ttttttaatt tttcaacttt ttttatttaa ctatgctaat
     4201 catattataa acattttaaa aatcaagtcc aactatttat gaatcattga agagacaaca
     4261 taattataac agtaaaaata ataataataa taataatttt gtcttattgt ttcaaacata
     4321 ttatgttcca taaatatcta aatttttaat atggtcataa attcatagga tttaatttat
     4381 ataaaatatg gctattcata ctagccattt tatgtgtgta aagttttttt tttctgaaca
     4441 aaaaataaaa tgttcataat tttagctatt tacctgcatg tgcatgtaca aaaaaaaata
     4501 tgcacacaac atacacattt ttacagttat aaatacaatc aattggtata aatatataag
     4561 taagaaagcg ctagcctaaa cacagtagta tctgtcacca aagtcaccca accctggaac
     4621 tagatacttg ttttcatcca gtcctctgtc gagggcacca gtaacaattc tgacctctgg
     4681 gaaggcggca tggtattttt caatcccttc cttactacag attaggttta agaagtaaat
     4741 tctctctggc ttaacacctc tcttaatcaa gacttctgta gccatgatag cactacctcc
     4801 ggttgccagc attgggtcta atagaaagac atacctttca gatatatcct ctggtaattt
     4861 ttcgtagaat aactttggta aagcagtctc ctcgtccctt tgaattaaaa ttttaccgat
     4921 acgcacagac ctacaacagt ctcttaatcc ttgctccatt gattcaccag ctctgacaat
     4981 ggaaacacca cagattttac ccatgaatga gacaccttca aagttttcgt tggtgtcagt
     5041 ttccacaatt tgcttttgca caggtagatg gttcaaacct tcttcaacca acaatctgat
     5101 gattctatcg gagtagaaaa tgaaatcagg tctagttgta ttcttatttc tgatgatggt
     5161 gtaaagaccc agcagttgat ttgtttgagg tagcaagtag acgttcttaa atggttccga
     5221 agcctcacca atatcttcaa accaatcttg aggtctttca tcaataaatt gtttcataat
     5281 ctttttacac ctctcatcat caacaacaac aacctcgtga cctctagttt gtaaatactt
     5341 ctcgccctta cttttgaaat tcacgttctc accgacaaca cagcgaggaa taccatacat
     5401 gatgattgca ccagtacaca tatcgcatgg agacagcgtc gtgtaaagag tggtatcttt
     5461 gtagactttg ccctctaatc tcccacagtt ttccaaagtg gagatttcac catgtagtgt
     5521 ggcggaaccc ttttgaaatc tcatgttgtg accacgaccg agaacacttc cgtctttgtt
     5581 attgataaga catccgccaa taggaacacc accctctttg taacccaagg ccgcctcctc
     5641 ataggcaatg tccataccct tctgatccca cttgcttgcc attccccctg tcacactagc
     5701 acttccacct ccaccagatc cacctcctcc actagcatca ttcttctcat atacttcaaa
     5761 tttgtactta atgcctttct cctcctgcac atcagagaga acacctgggt attctggcag
     5821 aagtttatat ttctccaaat caatttctgg aaaaaacgtg tcactttcaa agtcttgcat
     5881 gatccttgtc acaaatagtt taagatggcc tgggtgattc atggcttcct tataaacaga
     5941 actgccacca actatccaga ccatgtctac tttatttgct aattctggtt gttcagtaag
     6001 ttttaaggca tcatcgagac ttctggaaag aaaatgggct ccttgtggtg gttccttgag
     6061 ttctctgctg agaactaaat tgattctacc cttcaaaggt cgattcttct caggaatgga
     6121 gaaccaagtc tttttaccca taatcaccag attctgttta ccttctactg aagaggttgt
     6181 ggtcattctc tggaaatatc taaattcgtt cctgagcgga ggccagggca ggtctccgtt
     6241 cttgccgatg cccatgttct gagacacagc gacgatgcag tttagcgaac caaccatttt
     6301 gtaatctgca ggtgtgtatt tatatatata agcaatatac aaatgaaaat actattataa
     6361 aacggaaact caaaaaatgt tgaaacaaat agtcaatttt taaaatgaat gtgtaaattg
     6421 tttaagtttt atgacaaaaa aaggaaaagt gtatataaaa taaaaattgg aaaaaattta
     6481 tatattacat gccatataat accatttatt tactatttat atcttaatat aaaaaattgt
     6541 agcaaaaata tataatcgta ttttatatat ttaatattat ataacccctt aaaaaaaaaa
     6601 atttaattga aaaaatttta atttatgcac ttttttattg tattatatat attccgttat
     6661 tttaattaca cataaactta atagaaaaat taattcgctg ctaaataaaa attatagggc
     6721 tatatatgat gggatcatat tctatctttc tctttttcaa attctttata ttttagaata
     6781 caaatcgctt taaacaataa aaaagaggta tatcctaccc gaattgacac acacaataaa
     6841 taataaatat ataaataata aaaatataaa tatataaatt ataaatatat aaattatata
     6901 tataaatata ttaacatgtg tgtaatttgt taaaattgat attaaacagc tttgcatggg
     6961 gaaagagtgg cttttataag cgaatcatat aatacagtgt taattttaat atataaaata
     7021 taatttcctt aaattgttat ttaacatttt taattgccta cccaaggtaa tataatatcc
     7081 aaatgacaat caaagataaa tgaaataccg ctccattttt cctatatatc cattttcatt
     7141 ttgtgtaaat gttcataaaa ttttatcata tactcataaa aaacagggga aaaatgaaaa
     7201 aatgaacgtc caccccgtgt gaatatgctc attttgtata ctgatcagaa attgaaggaa
     7261 aaaacatcat ttgtgtttat atgttttttt ttaatttttc aacttttttt atttaactat
     7321 gctaatcata ttataaacat tttaaaaatc aagtccaact atttatgaat cattgaagag
     7381 acaacataat tataacagta aaaataataa taataataat aattttgtct tattgtttca
     7441 aacatattat gttccataaa tatctaaatt tttaatatgg tcataaattc ataggattta
     7501 atttatataa aatatggcta ttcatactag ccattttatg tgtgtaaagt ttttttttct
     7561 gaacaaaaaa taaaatgttc ataattttag ctatttacct gcatgtgcat gtacaaaaaa
     7621 aaatatgcac acaacataca catttttaca gttataaata caatcaattg gtataaatat
     7681 ataagtaaga aaaaacgcgt gggcccccta ggtacgtatc tcgagcccgg gaagcttatc
     7741 gatggtaccg tttaaacgaa gtacttcgtc gacgacgtcg tgcacgaccg atcgtccctt
     7801 tagtgagggt taatcatggt catagctgtt tcctgactcg ctgcgctcgg tcgttcggct
     7861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8401 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gtaacgttgt
     9061 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9121 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9181 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9241 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9301 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9361 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9421 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9481 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9541 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9601 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9661 tctcatgagc ggatacatat ttgaatgtat t
//
