LOCUS       62056_3655        6502 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(58..768)
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1748..1888
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2074..2662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2836..3693)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3694..3798)
                     /label=AmpR promoter
     promoter        3904..4005
                     /label=TRP1 promoter
     promoter        4100..4459
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     protein_bind    complement(5818..5851)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attctcaatt
       61 aagtttgtgc cccagtttgc tagggaggtc gcagtatctg gccacagcca cctcgtgctg
      121 ctcgacgtag gtctctttgt cggcctcctt gattctttcc agtctgtggt ccacatagta
      181 gacgccgggc atcttgaggt tcttagcggg tttcttggat ctgtatgtgg tcttgaagtt
      241 gcagatcagg tggcccccgc ccacgagctt cagggccatg tcgcttctgc cttccaggcc
      301 gccgtcagcg gggtacagca tctcggtgtt ggcctcccag ccgagtgttt tcttctgcat
      361 cacagggccg ttggatggga agttcacccc tctgatcttg acgttgtaga tgaggcagcc
      421 gtcctggagg ctggtgtcct gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac
      481 tctctcccat gtgaagccct cagggaagga ctgcttaaag aagtcgggga tgccctgggt
      541 gtggttgatg aaggttctgc tgccgtacat gaagctggta gccaggatgt cgaaggcgaa
      601 ggggagaggg ccgccctcga ccaccttgat tctcatggtc tgggtgccct cgtagggctt
      661 gccttcgccc tcggatgtgc acttgaagtg gtggttgttc acggtgccct ccatgtacag
      721 cttcatgtgc atgttctcct taatcagctc ttcgccctta gacaccatgg atcctctaga
      781 gtcgaggtcg acggtatcga taagcttgat taaaggttct tggatgggaa gatgaatata
      841 ctgaagatgg gaaaagaaag agaaaagaaa agagcagctg gtggggagag caggaaaata
      901 tggcaacaaa tgttggactg acgcaacgac cttgtcaacc ccgccgacac accgggcgga
      961 cagacggggc aaagctgcct accagggact gagggacctc agcaggtcga gtgcagagca
     1021 ccggatgggt cgactgccag cttgtgttcc cggtctgcgc cgctggccag ctcctgagcg
     1081 gcctttccgg tttcatacac cgggcaaagc aggagaggca cgatatttgg acgccctaca
     1141 gatgccggat gggccaatta gggagcttac gcgccgggta ctcgctctac ctacttcgga
     1201 gaaggtacta tctcgtgaat cttttaccag atcggaagca attggacttc tgtacctagg
     1261 ttaatggcat gctatttcgc cgacggctat acacccctgg cttcacattc tccttcgctt
     1321 actgccggtg attcgatgaa gctccatatt ctccgatgat gcaatagatt cttggtcaac
     1381 gaggggcaca ccagcctttc cacttcgggg cggaggggcg gccggtcccg gattaataat
     1441 catccactgc acctcagagc cgccagagct gtctggcgca gtggcgctta ttactcagcc
     1501 cttctctctg cgtccgtccg tctctccgca tgctttgcat acttctgcct gctggggagc
     1561 ctggggactt tccacaccct aactgacaca cattccacag agatctctgc ctcgcgcgtt
     1621 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     1681 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     1741 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     1801 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     1861 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     1921 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1981 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2041 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2101 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2161 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2221 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2281 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2341 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2401 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2461 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2521 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2581 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2641 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2701 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2761 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2821 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2881 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2941 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3001 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3061 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3121 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3181 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3241 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3301 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3361 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3421 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3481 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3541 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3601 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3661 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3721 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3781 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     3841 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     3901 attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
     3961 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat
     4021 ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca
     4081 gaatgtgctc tagactcgac agaagatgat attgaaggag cactttttgg gcttggctgg
     4141 agctagtgga ggtcaacaat gaatgcctat tttggtttag tcgtccaggc ggtgagcaca
     4201 aaatttgtgt cgtttgacaa gatggttcat ttaggcaact ggtcagatca gccccacttg
     4261 tagcagtagc ggcggcgctc gaagtgtgac tcttattagc agacaggaac gaggacatta
     4321 ttatcatctg ctgcttggtg cacgataact tggtgcgttt gtcaagcaag gtaagtgaac
     4381 gacccggtca taccttctta agttcgccct tcctcccttt atttcagatt caatctgact
     4441 tacctattct acccaagcaa agcttcgatt aggaagtaac catgtcgtcc aagtcgcaat
     4501 tgacctactc cgcccgtgcc agcaagcacc ccaatgcgct ggtgaagaag ctcttcgaga
     4561 tcgccgaggc gaagaagacc aacgtcaccg tgtccgccga tgtcacgacc accaaggagc
     4621 tccttgatct ggcggaccgc ctcggccctc acatcgccgt gatcaaaacc cacatcgaca
     4681 tcctctccga cttcagcgag gaaaccacca ccggcctgaa agccctcgcc gagaagcaca
     4741 acttcctcat cttcgaagac cgcaagttca tcgacatcgg caacaccgtc cagaagcagt
     4801 accacggcgg tgtcctccgc atctccgaat gggcccacat catcaattgc gccatcctcc
     4861 cgggcgaggg catcgtcgaa gccctcgccc agaccgccgc cgccgccgac ttcccctacg
     4921 gctcggagcg cggcctgctc atcctcgcgg agatgacctc gaagggctcg cttgcgacgg
     4981 gccagtacac gacctcgtcg gtggactacg cgcgcaaata caagaagttt gtgatgggct
     5041 tcgtgtcgac ccggtcgctg ggcgaggtcg agtcggcggt gtcggagcga tcagaggagg
     5101 aggactttgt ggtgttcacg acgggagtga atctctcgtc gaagggggat aagttggggc
     5161 agcaatacca gacgcctgag tcggcgattg ggcgtggtgc ggatttcatt attgcgggtc
     5221 ggggcatcta tgcggctcct gatccggttg aggccgtgca gcggtaccag aaggaggggt
     5281 gggaggcgta ccaggcgcgc gtggcgggtc agtagtccgt cgacctgcag atcgttcaaa
     5341 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5401 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tagtaccgag
     5461 aaactagtgg atctaaacat aaaatctgta aaataacaag atgtaaagat aatgctaaat
     5521 catttggctt tttgattgat tgtacaggaa aatatacatc gcagggggtt gacttttacc
     5581 atttcaccgc aatggaatca aacttgttga agagaatgtt cacaggcgca tacgctacaa
     5641 tgacccgatt cttgctagcc ttttctcggt cttgcaaaca accgccgcgg ccggtcgagg
     5701 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccggatcgcg
     5761 gccgctcgac ctgcagccaa gctagcttgg ctggacgtaa actcctcttc agacctaata
     5821 acttcgtata gcatacatta tacgaagtta tattaagggt tattgaatat gatcggaatt
     5881 ggtcgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac ggcagaagca
     5941 cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt cattgaaaga
     6001 aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat agcaaagatt
     6061 gaataaggcg catttttctt caaagcttgc aaattaaagc cttcgagcgt cccaaaacct
     6121 tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgctatatt ttgttttcta
     6181 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
     6241 tcatgcattg cagatgagct gtatctggaa gaggtaaacc cgaaacgcgt tttattcttg
     6301 ttgacatgga gctattaaat cactagaagg cactctttgc tgcttggaca aatgaacgta
     6361 tcttatcgag atcctgaaca ccatttgtct caactccgga gctgacatcg acaccaacga
     6421 tcttatatcc agattcgtca agctgtttga tgatttcagt aacgttaagt ggatctcggt
     6481 gacgggcagg accggacggg gc
//
