LOCUS       62056_3650        6508 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(58..774)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1754..1894
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2080..2668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2842..3699)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3700..3804)
                     /label=AmpR promoter
     promoter        3910..4011
                     /label=TRP1 promoter
     promoter        4106..4465
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     protein_bind    complement(5824..5857)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcttactt
       61 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      121 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
      181 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      241 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
      301 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
      361 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      421 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
      481 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      541 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
      601 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
      661 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
      721 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggatcc
      781 tctagagtcg aggtcgacgg tatcgataag cttgattaaa ggttcttgga tgggaagatg
      841 aatatactga agatgggaaa agaaagagaa aagaaaagag cagctggtgg ggagagcagg
      901 aaaatatggc aacaaatgtt ggactgacgc aacgaccttg tcaaccccgc cgacacaccg
      961 ggcggacaga cggggcaaag ctgcctacca gggactgagg gacctcagca ggtcgagtgc
     1021 agagcaccgg atgggtcgac tgccagcttg tgttcccggt ctgcgccgct ggccagctcc
     1081 tgagcggcct ttccggtttc atacaccggg caaagcagga gaggcacgat atttggacgc
     1141 cctacagatg ccggatgggc caattaggga gcttacgcgc cgggtactcg ctctacctac
     1201 ttcggagaag gtactatctc gtgaatcttt taccagatcg gaagcaattg gacttctgta
     1261 cctaggttaa tggcatgcta tttcgccgac ggctatacac ccctggcttc acattctcct
     1321 tcgcttactg ccggtgattc gatgaagctc catattctcc gatgatgcaa tagattcttg
     1381 gtcaacgagg ggcacaccag cctttccact tcggggcgga ggggcggccg gtcccggatt
     1441 aataatcatc cactgcacct cagagccgcc agagctgtct ggcgcagtgg cgcttattac
     1501 tcagcccttc tctctgcgtc cgtccgtctc tccgcatgct ttgcatactt ctgcctgctg
     1561 gggagcctgg ggactttcca caccctaact gacacacatt ccacagagat ctctgcctcg
     1621 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     1681 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     1741 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     1801 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     1861 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     1921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2521 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3841 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     3901 tcaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
     3961 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
     4021 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
     4081 gccgcagaat gtgctctaga ctcgacagaa gatgatattg aaggagcact ttttgggctt
     4141 ggctggagct agtggaggtc aacaatgaat gcctattttg gtttagtcgt ccaggcggtg
     4201 agcacaaaat ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc
     4261 cacttgtagc agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg
     4321 acattattat catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa
     4381 gtgaacgacc cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat
     4441 ctgacttacc tattctaccc aagcaaagct tcgattagga agtaaccatg tcgtccaagt
     4501 cgcaattgac ctactccgcc cgtgccagca agcaccccaa tgcgctggtg aagaagctct
     4561 tcgagatcgc cgaggcgaag aagaccaacg tcaccgtgtc cgccgatgtc acgaccacca
     4621 aggagctcct tgatctggcg gaccgcctcg gccctcacat cgccgtgatc aaaacccaca
     4681 tcgacatcct ctccgacttc agcgaggaaa ccaccaccgg cctgaaagcc ctcgccgaga
     4741 agcacaactt cctcatcttc gaagaccgca agttcatcga catcggcaac accgtccaga
     4801 agcagtacca cggcggtgtc ctccgcatct ccgaatgggc ccacatcatc aattgcgcca
     4861 tcctcccggg cgagggcatc gtcgaagccc tcgcccagac cgccgccgcc gccgacttcc
     4921 cctacggctc ggagcgcggc ctgctcatcc tcgcggagat gacctcgaag ggctcgcttg
     4981 cgacgggcca gtacacgacc tcgtcggtgg actacgcgcg caaatacaag aagtttgtga
     5041 tgggcttcgt gtcgacccgg tcgctgggcg aggtcgagtc ggcggtgtcg gagcgatcag
     5101 aggaggagga ctttgtggtg ttcacgacgg gagtgaatct ctcgtcgaag ggggataagt
     5161 tggggcagca ataccagacg cctgagtcgg cgattgggcg tggtgcggat ttcattattg
     5221 cgggtcgggg catctatgcg gctcctgatc cggttgaggc cgtgcagcgg taccagaagg
     5281 aggggtggga ggcgtaccag gcgcgcgtgg cgggtcagta gtccgtcgac ctgcagatcg
     5341 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     5401 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatagt
     5461 accgagaaac tagtggatct aaacataaaa tctgtaaaat aacaagatgt aaagataatg
     5521 ctaaatcatt tggctttttg attgattgta caggaaaata tacatcgcag ggggttgact
     5581 tttaccattt caccgcaatg gaatcaaact tgttgaagag aatgttcaca ggcgcatacg
     5641 ctacaatgac ccgattcttg ctagcctttt ctcggtcttg caaacaaccg ccgcggccgg
     5701 tcgagggacc taataacttc gtatagcata cattatacga agttatatta agggttccgg
     5761 atcgcggccg ctcgacctgc agccaagcta gcttggctgg acgtaaactc ctcttcagac
     5821 ctaataactt cgtatagcat acattatacg aagttatatt aagggttatt gaatatgatc
     5881 ggaattggtc gatcaggtat tgctgttatc tgatgagtat acgttgtcct ggccacggca
     5941 gaagcacgct tatcgctcca atttcccaca acattagtca actccgttag gcccttcatt
     6001 gaaagaaatg aggtcatcaa atgtcttcca atgtgagatt ttgggccatt ttttatagca
     6061 aagattgaat aaggcgcatt tttcttcaaa gcttgcaaat taaagccttc gagcgtccca
     6121 aaaccttctc aagcaaggtt ttcagtataa tgttacatgc gtacacgcgc tatattttgt
     6181 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
     6241 ataacgtcat gcattgcaga tgagctgtat ctggaagagg taaacccgaa acgcgtttta
     6301 ttcttgttga catggagcta ttaaatcact agaaggcact ctttgctgct tggacaaatg
     6361 aacgtatctt atcgagatcc tgaacaccat ttgtctcaac tccggagctg acatcgacac
     6421 caacgatctt atatccagat tcgtcaagct gtttgatgat ttcagtaacg ttaagtggat
     6481 ctcggtgacg ggcaggaccg gacggggc
//
