LOCUS       62056_3640        5992 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1046..1186
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1372..1960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2134..2991)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2992..3096)
                     /label=AmpR promoter
     promoter        3202..3303
                     /label=TRP1 promoter
     promoter        3398..3757
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             3780..4802
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    complement(5308..5341)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
       61 cccgggggat cctctagagt cgaggtcgac ggtatcgata agcttgatta aaggttcttg
      121 gatgggaaga tgaatatact gaagatggga aaagaaagag aaaagaaaag agcagctggt
      181 ggggagagca ggaaaatatg gcaacaaatg ttggactgac gcaacgacct tgtcaacccc
      241 gccgacacac cgggcggaca gacggggcaa agctgcctac cagggactga gggacctcag
      301 caggtcgagt gcagagcacc ggatgggtcg actgccagct tgtgttcccg gtctgcgccg
      361 ctggccagct cctgagcggc ctttccggtt tcatacaccg ggcaaagcag gagaggcacg
      421 atatttggac gccctacaga tgccggatgg gccaattagg gagcttacgc gccgggtact
      481 cgctctacct acttcggaga aggtactatc tcgtgaatct tttaccagat cggaagcaat
      541 tggacttctg tacctaggtt aatggcatgc tatttcgccg acggctatac acccctggct
      601 tcacattctc cttcgcttac tgccggtgat tcgatgaagc tccatattct ccgatgatgc
      661 aatagattct tggtcaacga ggggcacacc agcctttcca cttcggggcg gaggggcggc
      721 cggtcccgga ttaataatca tccactgcac ctcagagccg ccagagctgt ctggcgcagt
      781 ggcgcttatt actcagccct tctctctgcg tccgtccgtc tctccgcatg ctttgcatac
      841 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagag
      901 atctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      961 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     1021 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     1081 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     1141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     1201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3121 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     3181 gccctttcgt cttcaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga
     3241 gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg
     3301 agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg
     3361 cagagcacag aggccgcaga atgtgctcta gactcgacag aagatgatat tgaaggagca
     3421 ctttttgggc ttggctggag ctagtggagg tcaacaatga atgcctattt tggtttagtc
     3481 gtccaggcgg tgagcacaaa atttgtgtcg tttgacaaga tggttcattt aggcaactgg
     3541 tcagatcagc cccacttgta gcagtagcgg cggcgctcga agtgtgactc ttattagcag
     3601 acaggaacga ggacattatt atcatctgct gcttggtgca cgataacttg gtgcgtttgt
     3661 caagcaaggt aagtgaacga cccggtcata ccttcttaag ttcgcccttc ctccctttat
     3721 ttcagattca atctgactta cctattctac ccaagcaaag cttcgattag gaagtaacca
     3781 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     3841 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     3901 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     3961 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     4021 gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     4081 aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag gctatggatg
     4141 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     4201 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     4261 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     4321 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     4381 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     4441 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     4501 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac
     4561 gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     4621 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     4681 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     4741 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaaga
     4801 aatagtccgt cgacctgcag atcgttcaaa catttggcaa taaagtttct taagattgaa
     4861 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     4921 aataattaac atgtaatgca tagtaccgag aaactagtgg atctaaacat aaaatctgta
     4981 aaataacaag atgtaaagat aatgctaaat catttggctt tttgattgat tgtacaggaa
     5041 aatatacatc gcagggggtt gacttttacc atttcaccgc aatggaatca aacttgttga
     5101 agagaatgtt cacaggcgca tacgctacaa tgacccgatt cttgctagcc ttttctcggt
     5161 cttgcaaaca accgccgcgg ccggtcgagg gacctaataa cttcgtatag catacattat
     5221 acgaagttat attaagggtt ccggatcgcg gccgctcgac ctgcagccaa gctagcttgg
     5281 ctggacgtaa actcctcttc agacctaata acttcgtata gcatacatta tacgaagtta
     5341 tattaagggt tattgaatat gatcggaatt ggtcgatcag gtattgctgt tatctgatga
     5401 gtatacgttg tcctggccac ggcagaagca cgcttatcgc tccaatttcc cacaacatta
     5461 gtcaactccg ttaggccctt cattgaaaga aatgaggtca tcaaatgtct tccaatgtga
     5521 gattttgggc cattttttat agcaaagatt gaataaggcg catttttctt caaagcttgc
     5581 aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt ataatgttac
     5641 atgcgtacac gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
     5701 taatcataaa aacccatctc ataaataacg tcatgcattg cagatgagct gtatctggaa
     5761 gaggtaaacc cgaaacgcgt tttattcttg ttgacatgga gctattaaat cactagaagg
     5821 cactctttgc tgcttggaca aatgaacgta tcttatcgag atcctgaaca ccatttgtct
     5881 caactccgga gctgacatcg acaccaacga tcttatatcc agattcgtca agctgtttga
     5941 tgatttcagt aacgttaagt ggatctcggt gacgggcagg accggacggg gc
//
