LOCUS       62056_3635        8743 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    652..685
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1191..2213)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(2236..2595)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(2690..2791)
                     /label=TRP1 promoter
     promoter        2897..3001
                     /label=AmpR promoter
     CDS             3002..3859
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4033..4621
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4807..4947)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8710..8726)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccccgtccg gtcctgcccg tcaccgagat ccacttaacg ttactgaaat catcaaacag
       61 cttgacgaat ctggatataa gatcgttggt gtcgatgtca gctccggagt tgagacaaat
      121 ggtgttcagg atctcgataa gatacgttca tttgtccaag cagcaaagag tgccttctag
      181 tgatttaata gctccatgtc aacaagaata aaacgcgttt cgggtttacc tcttccagat
      241 acagctcatc tgcaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
      301 caattataca tttaatacgc gatagaaaac aaaatatagc gcgtgtacgc atgtaacatt
      361 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaagctt
      421 tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg
      481 gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg
      541 tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga
      601 taacagcaat acctgatcga ccaattccga tcatattcaa taacccttaa tataacttcg
      661 tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagcta
      721 gcttggctgc aggtcgagcg gccgcgatcc ggaaccctta atataacttc gtataatgta
      781 tgctatacga agttattagg tccctcgacc ggccgcggcg gttgtttgca agaccgagaa
      841 aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag
      901 tttgattcca ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta
      961 caatcaatca aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt
     1021 ttatgtttag atccactagt ttctcggtac tatgcattac atgttaatta ttacatgctt
     1081 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     1141 taagaaactt tattgccaaa tgtttgaacg atctgcaggt cgacggacta tttctttgcc
     1201 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
     1261 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
     1321 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
     1381 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag tcgtggcgat
     1441 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
     1501 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
     1561 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
     1621 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
     1681 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
     1741 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
     1801 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata
     1861 gcctccgcga ccggttgtag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
     1921 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctaaactc cccaatgtca
     1981 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
     2041 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
     2101 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
     2161 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catggttact
     2221 tcctaatcga agctttgctt gggtagaata ggtaagtcag attgaatctg aaataaaggg
     2281 aggaagggcg aacttaagaa ggtatgaccg ggtcgttcac ttaccttgct tgacaaacgc
     2341 accaagttat cgtgcaccaa gcagcagatg ataataatgt cctcgttcct gtctgctaat
     2401 aagagtcaca cttcgagcgc cgccgctact gctacaagtg gggctgatct gaccagttgc
     2461 ctaaatgaac catcttgtca aacgacacaa attttgtgct caccgcctgg acgactaaac
     2521 caaaataggc attcattgtt gacctccact agctccagcc aagcccaaaa agtgctcctt
     2581 caatatcatc ttctgtcgag tctagagcac attctgcggc ctctgtgctc tgcaagccgc
     2641 aaactttcac caatggacca gaactacctg tgaaattaat aacagacata ctccaagctg
     2701 cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc cctccctctt
     2761 ggccctctcc ttttcttttt tcgaccgaat taattcttga agacgaaagg gcctcgtgat
     2821 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2881 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2941 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3001 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3061 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3121 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3181 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3241 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3301 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3361 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3421 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3481 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3541 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3601 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3661 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3721 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3781 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3841 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3901 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3961 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4021 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4081 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4141 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4201 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4261 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4321 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4381 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4441 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4501 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4561 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4621 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4681 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4741 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4801 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     4861 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     4921 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     4981 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5041 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagag atctctgtgg
     5101 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5161 agcatgcgga gagacggacg gacgcagaga gaagggctga gtaataagcg ccactgcgcc
     5221 agacagctct ggcggctctg aggtgcagtg gatgattatt aatccgggac cggccgcccc
     5281 tccgccccga agtggaaagg ctggtgtgcc cctcgttgac caagaatcta ttgcatcatc
     5341 ggagaatatg gagcttcatc gaatcaccgg cagtaagcga aggagaatgt gaagccaggg
     5401 gtgtatagcc gtcggcgaaa tagcatgcca ttaacctagg tacagaagtc caattgcttc
     5461 cgatctggta aaagattcac gagatagtac cttctccgaa gtaggtagag cgagtacccg
     5521 gcgcgtaagc tccctaattg gcccatccgg catctgtagg gcgtccaaat atcgtgcctc
     5581 tcctgctttg cccggtgtat gaaaccggaa aggccgctca ggagctggcc agcggcgcag
     5641 accgggaaca caagctggca gtcgacccat ccggtgctct gcactcgacc tgctgaggtc
     5701 cctcagtccc tggtaggcag ctttgccccg tctgtccgcc cggtgtgtcg gcggggttga
     5761 caaggtcgtt gcgtcagtcc aacatttgtt gccatatttt cctgctctcc ccaccagctg
     5821 ctcttttctt ttctctttct tttcccatct tcagtatatt catcttccca tccaagaacc
     5881 tttaatcaag cttatcgata ccgtcgacct cgactctaga ggatccatgt tgtccaaccc
     5941 attgagaaga tactctgcct acccagacat ctcctctgct tctttcgacc caaactacca
     6001 cggttctcaa tctcacttgc actctatcaa cgtcaacact ttcggtaact ctcacccata
     6061 cccaatgcaa cacttggctc aacacgctga attgtcttct tctagaatga tcagagcctc
     6121 tccagttcaa ccaaagcaaa gacaaggttc tttgattgct gccagaaaga actctactgg
     6181 tactgctggt ccaattagaa gaagaatctc tagagcttgt gaccaatgta accaattgag
     6241 aaccaagtgt gatggtttgc acccatgtgc ccactgtatc gaatttggtt tgggttgtga
     6301 atacgtcaga gaaagaaaga agagaggtaa ggcttctaga aaggatattg ctgcccaaca
     6361 agccgctgct gctgctgctc aacactccgg tcaagtccaa gatggtccag aagatcaaca
     6421 cagaaagttg tctagacaac aatctgaatc ttctagaggt tctgctgaat tggcccaacc
     6481 agcccacgac ccaccacacg gtcacattga aggttctgtc tcttccttct ctgacaacgg
     6541 tttgtcccaa cacgctgcca tgggtggtat ggatggtttg gaagatcacc acggtcacgt
     6601 cggtgttgat ccagccttgg gtagaactca attggaagct tcttctgcta tgggtttggg
     6661 tgcttacggt gaagtccacc caggttacga atctccaggt atgaacggtc acgttatggt
     6721 tccaccatct tacggtgctc aaaccaccat ggccggttac tccggtatct cttacgctgc
     6781 tcaagcccca tctccagcta cttactcttc tgacggtaac ttcagattga ccggtcacat
     6841 ccacgattac ccattggcta acggttcttc tccatcttgg ggtcaatctg atttgagata
     6901 cccagttttg gaaccattgt tgccacactt gggtaacata ctcccagtga gcttggcttg
     6961 tgatttgatt gacttgtact tctcctcttc ttcttctgct caaatgcacc caatgtcccc
     7021 atacgttctc ggcttcgtct tcagaaagag atccttcttg cacccaacta acccaagaag
     7081 atgtcaacca gctttgttgg cttctatgtt gtgggttgct gctcaaactt ctgaagcttc
     7141 cttcttgact tctttgccat ctgctagatc taaggtctgt caaaagttgt tggaattgac
     7201 cgttggtttg ttgcaaccat tgatccacac cggtaccaac tctccatctc caaagacttc
     7261 tccagtcgtc ggtgctgctg ccttgggtgt tttgggtgtt gccatgccag gttctctcaa
     7321 catggatagc ttggccggcg aaactggtgc gttcggtgcc atagggagct tggacgacgt
     7381 cataacctac gttcacttgg ccactgtcgt ctctgcctct gaatacaagg gtgcctcttt
     7441 gagatggtgg ggtgctgctt ggtctttggc cagagaattg aagttgggta gagaattgcc
     7501 accaggtaac ccaccagcca accaagaaga cggtgaaggt ttgtctgaag acgttgatga
     7561 acacgacttg aacagaaaca acactagatt cgttactgaa gaagaaagag aagaaagaag
     7621 aagagcttgg tggttggttt acatcgtcga cagacacttg gctttgtgtt acaacagacc
     7681 attgttcttg ttggactctg aatgttctga cttgtaccac ccaatggacg acatcaagtg
     7741 gcaagctggt aagttcagat ctcacgatgc tggtaactcc tctatcaaca tcgattcttc
     7801 catgactgac gaatttggtg attctccaag agctgctaga ggtgctcact acgaatgtag
     7861 aggtagatct attttcggtt acttcttgtc cttgatgact atcttgggtg aaattgtcga
     7921 tgtccaccac gctaagtctc acccaagatt tggcgttggc ttcagatccg ctcgtgattg
     7981 ggacgaacaa gttgctgaaa tcaccagaca cttggacatg tacgaagaat ctttgaagag
     8041 attcgttgcc aagcacttgc cattgtcctc taaggacaag gaacaacacg aaatgcacga
     8101 ctctggtgct gttactgaca tgcaatctcc attgtctgtt agaaccaacg cttcctctag
     8161 aatgactgaa tctgaaatcc aagcctctat cgttgttgct tactctaccc acgttatgca
     8221 cgtcttgcac atcttgttgg ctgataagtg ggacccaatc aacttgttgg acgacgacga
     8281 cttgtggatc tcttctgaag gtttcgttac tgctacttct cacgctgtta gtgctgcgga
     8341 agctataagc caaattttgg aatttgaccc aggtttggaa tttatgccat tcttctacgg
     8401 tgtctacttg ttgcaaggtt ccttcttgtt gttgttgatc gccgacaagt tgcaagccga
     8461 agcttctcca tctgtcatca aggcttgtga aaccattgtt agagctcacg aagcttgtgt
     8521 tgttactttg tctactgaat accaaagaaa cttctctaag gttatgagat ctgctttggc
     8581 tttgattaga ggtagagttc cagaagattt ggctgaacaa caacaaagaa gaagagaatt
     8641 gttggctttg tacagatgga ctggtaacgg cactggtctc gccttgtaag aattcgatat
     8701 caagcttatc gataccgtcg acctcgaggg ggggcccggt acc
//
