LOCUS       62056_3630        6690 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(57..764)
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    1744..1884
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2070..2658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2832..3689)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3690..3794)
                     /label=AmpR promoter
     promoter        3900..4001
                     /label=TRP1 promoter
     promoter        4096..4455
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             4478..5500
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    complement(6006..6039)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attctacttg
       61 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
      121 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
      181 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
      241 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
      301 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
      361 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
      421 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      481 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      541 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
      601 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
      661 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
      721 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggatcc tctagagtcg
      781 aggtcgacgg tatcgataag cttgattaaa ggttcttgga tgggaagatg aatatactga
      841 agatgggaaa agaaagagaa aagaaaagag cagctggtgg ggagagcagg aaaatatggc
      901 aacaaatgtt ggactgacgc aacgaccttg tcaaccccgc cgacacaccg ggcggacaga
      961 cggggcaaag ctgcctacca gggactgagg gacctcagca ggtcgagtgc agagcaccgg
     1021 atgggtcgac tgccagcttg tgttcccggt ctgcgccgct ggccagctcc tgagcggcct
     1081 ttccggtttc atacaccggg caaagcagga gaggcacgat atttggacgc cctacagatg
     1141 ccggatgggc caattaggga gcttacgcgc cgggtactcg ctctacctac ttcggagaag
     1201 gtactatctc gtgaatcttt taccagatcg gaagcaattg gacttctgta cctaggttaa
     1261 tggcatgcta tttcgccgac ggctatacac ccctggcttc acattctcct tcgcttactg
     1321 ccggtgattc gatgaagctc catattctcc gatgatgcaa tagattcttg gtcaacgagg
     1381 ggcacaccag cctttccact tcggggcgga ggggcggccg gtcccggatt aataatcatc
     1441 cactgcacct cagagccgcc agagctgtct ggcgcagtgg cgcttattac tcagcccttc
     1501 tctctgcgtc cgtccgtctc tccgcatgct ttgcatactt ctgcctgctg gggagcctgg
     1561 ggactttcca caccctaact gacacacatt ccacagagat ctctgcctcg cgcgtttcgg
     1621 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     1681 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     1741 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     1801 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     1861 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1921 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1981 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2701 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2761 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2821 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2881 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2941 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3001 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3061 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3121 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3181 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3241 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3301 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3361 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3421 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3481 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3541 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3601 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3661 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3721 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3781 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3841 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta
     3901 attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga ctattgagca
     3961 cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt attaatttca
     4021 caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag gccgcagaat
     4081 gtgctctaga ctcgacagaa gatgatattg aaggagcact ttttgggctt ggctggagct
     4141 agtggaggtc aacaatgaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat
     4201 ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc
     4261 agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat
     4321 catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc
     4381 cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc
     4441 tattctaccc aagcaaagct tcgattagga agtaaccatg aaaaagcctg aactcaccgc
     4501 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     4561 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     4621 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     4681 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     4741 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     4801 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     4861 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     4921 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     4981 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     5041 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     5101 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     5161 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     5221 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     5281 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     5341 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     5401 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     5461 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagtccgtcg acctgcagat
     5521 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     5581 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcata
     5641 gtaccgagaa actagtggat ctaaacataa aatctgtaaa ataacaagat gtaaagataa
     5701 tgctaaatca tttggctttt tgattgattg tacaggaaaa tatacatcgc agggggttga
     5761 cttttaccat ttcaccgcaa tggaatcaaa cttgttgaag agaatgttca caggcgcata
     5821 cgctacaatg acccgattct tgctagcctt ttctcggtct tgcaaacaac cgccgcggcc
     5881 ggtcgaggga cctaataact tcgtatagca tacattatac gaagttatat taagggttcc
     5941 ggatcgcggc cgctcgacct gcagccaagc tagcttggct ggacgtaaac tcctcttcag
     6001 acctaataac ttcgtatagc atacattata cgaagttata ttaagggtta ttgaatatga
     6061 tcggaattgg tcgatcaggt attgctgtta tctgatgagt atacgttgtc ctggccacgg
     6121 cagaagcacg cttatcgctc caatttccca caacattagt caactccgtt aggcccttca
     6181 ttgaaagaaa tgaggtcatc aaatgtcttc caatgtgaga ttttgggcca ttttttatag
     6241 caaagattga ataaggcgca tttttcttca aagcttgcaa attaaagcct tcgagcgtcc
     6301 caaaaccttc tcaagcaagg ttttcagtat aatgttacat gcgtacacgc gctatatttt
     6361 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     6421 aaataacgtc atgcattgca gatgagctgt atctggaaga ggtaaacccg aaacgcgttt
     6481 tattcttgtt gacatggagc tattaaatca ctagaaggca ctctttgctg cttggacaaa
     6541 tgaacgtatc ttatcgagat cctgaacacc atttgtctca actccggagc tgacatcgac
     6601 accaacgatc ttatatccag attcgtcaag ctgtttgatg atttcagtaa cgttaagtgg
     6661 atctcggtga cgggcaggac cggacggggc
//
