LOCUS       62056_3615        6226 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(58..774)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1754..1894
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2080..2668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2842..3699)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3700..3804)
                     /label=AmpR promoter
     promoter        3910..4011
                     /label=TRP1 promoter
     promoter        4106..4465
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             4488..5036
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    complement(5542..5575)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcttactt
       61 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      121 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
      181 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      241 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
      301 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
      361 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      421 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
      481 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      541 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
      601 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
      661 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
      721 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggatcc
      781 tctagagtcg aggtcgacgg tatcgataag cttgattaaa ggttcttgga tgggaagatg
      841 aatatactga agatgggaaa agaaagagaa aagaaaagag cagctggtgg ggagagcagg
      901 aaaatatggc aacaaatgtt ggactgacgc aacgaccttg tcaaccccgc cgacacaccg
      961 ggcggacaga cggggcaaag ctgcctacca gggactgagg gacctcagca ggtcgagtgc
     1021 agagcaccgg atgggtcgac tgccagcttg tgttcccggt ctgcgccgct ggccagctcc
     1081 tgagcggcct ttccggtttc atacaccggg caaagcagga gaggcacgat atttggacgc
     1141 cctacagatg ccggatgggc caattaggga gcttacgcgc cgggtactcg ctctacctac
     1201 ttcggagaag gtactatctc gtgaatcttt taccagatcg gaagcaattg gacttctgta
     1261 cctaggttaa tggcatgcta tttcgccgac ggctatacac ccctggcttc acattctcct
     1321 tcgcttactg ccggtgattc gatgaagctc catattctcc gatgatgcaa tagattcttg
     1381 gtcaacgagg ggcacaccag cctttccact tcggggcgga ggggcggccg gtcccggatt
     1441 aataatcatc cactgcacct cagagccgcc agagctgtct ggcgcagtgg cgcttattac
     1501 tcagcccttc tctctgcgtc cgtccgtctc tccgcatgct ttgcatactt ctgcctgctg
     1561 gggagcctgg ggactttcca caccctaact gacacacatt ccacagagat ctctgcctcg
     1621 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     1681 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     1741 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     1801 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     1861 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     1921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2521 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3841 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     3901 tcaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
     3961 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
     4021 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
     4081 gccgcagaat gtgctctaga ctcgacagaa gatgatattg aaggagcact ttttgggctt
     4141 ggctggagct agtggaggtc aacaatgaat gcctattttg gtttagtcgt ccaggcggtg
     4201 agcacaaaat ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc
     4261 cacttgtagc agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg
     4321 acattattat catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa
     4381 gtgaacgacc cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat
     4441 ctgacttacc tattctaccc aagcaaagct tcgattagga agtaaccatg agcccagaac
     4501 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
     4561 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
     4621 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
     4681 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
     4741 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca
     4801 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
     4861 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
     4921 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
     4981 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatctgat
     5041 ccgtcgacct gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5101 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     5161 taacatgtaa tgcatagtac cgagaaacta gtggatctaa acataaaatc tgtaaaataa
     5221 caagatgtaa agataatgct aaatcatttg gctttttgat tgattgtaca ggaaaatata
     5281 catcgcaggg ggttgacttt taccatttca ccgcaatgga atcaaacttg ttgaagagaa
     5341 tgttcacagg cgcatacgct acaatgaccc gattcttgct agccttttct cggtcttgca
     5401 aacaaccgcc gcggccggtc gagggaccta ataacttcgt atagcataca ttatacgaag
     5461 ttatattaag ggttccggat cgcggccgct cgacctgcag ccaagctagc ttggctggac
     5521 gtaaactcct cttcagacct aataacttcg tatagcatac attatacgaa gttatattaa
     5581 gggttattga atatgatcgg aattggtcga tcaggtattg ctgttatctg atgagtatac
     5641 gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac
     5701 tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt
     5761 gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc ttgcaaatta
     5821 aagccttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
     5881 acacgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
     5941 taaaaaccca tctcataaat aacgtcatgc attgcagatg agctgtatct ggaagaggta
     6001 aacccgaaac gcgttttatt cttgttgaca tggagctatt aaatcactag aaggcactct
     6061 ttgctgcttg gacaaatgaa cgtatcttat cgagatcctg aacaccattt gtctcaactc
     6121 cggagctgac atcgacacca acgatcttat atccagattc gtcaagctgt ttgatgattt
     6181 cagtaacgtt aagtggatct cggtgacggg caggaccgga cggggc
//
