LOCUS       40924_5909        3781 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Yadong H, Chunfang X, Dongsheng Y.
  TITLE     Biobrick parts and vectors for Heterologous protein production using
            the Pichia pastoris
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Yadong H, Chunfang X, Dongsheng Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2013) Institute of Microbial Biotechnology, 
            Institute of Biomedicine, Jinan University, No. 601, Huangpu, 
            Guangzhou, Guangdong 510632, China
REFERENCE   3  (bases 1 to 3781)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2013) Institute of Microbial Biotechnology, Institute of 
            Biomedicine, Jinan University, No. 601, Huangpu, Guangzhou, 
            Guangdong 510632, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     Name: the biobrick plasmid backbone, pBAN vector.
FEATURES             Location/Qualifiers
     source          1..3781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     regulatory      22..890
                     /note="derived from Aspergillus Niger glucoamylase gene;
                     Tgla"
                     /regulatory_class="terminator"
     misc_feature    951..1258
                     /note="5' upstream sequence Aspergillus Niger glucoamylase 
                     promoter"
     promoter        1290..1394
                     /label=AmpR promoter
     CDS             1395..2252
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2426..3014
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3302..3323
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3338..3368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3376..3392
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3400..3416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3447..3758
                     /note="3' upstream sequence of Aspergillus Niger
                     glucoamylase promoter"
     misc_feature    3760..3781
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 tactagtagc ggccgctgca gacaatcaat ccatttcgct atagttaaag gatggggatg
       61 agggcaattg gttatatgat catgtatgta gtgggtgtgc ataatagtag tgaaatggaa
      121 gccaagtcat gtgattgtaa tcgaccgacg gaattgagga tatccggaaa tacagacacc
      181 gtgaaagcca tggtctttcc ttcgtgtaga agaccagaca gacagtccct gatttaccct
      241 tgcacaaagc actagaaaat tagcattcca tccttctctg cttgctctgc tgatatcact
      301 gtcattcaat gcatagccat gagctcatct tagatccaag cacgtaattc catagccgag
      361 gtccacagtg gagcagcaac attccccatc attgctttcc ccaggggcct cccaacgact
      421 aaatcaagag tatatctcta ccgtccaata gatcgtcttc gcttcaaaat ctttgacaat
      481 tccaagaggg tccccatcca tcaaacccag ttcaataata gccgagatgc atggtggagt
      541 caattaggca gtattgctgg aatgtcgggg ccagttggcc cggtggtcat tggccgcctg
      601 tgatgccatc tgccactaaa tccgatcatt gatccaccgc ccacgaggcg cgtctttgct
      661 ttttgcgcgg cgtccaggtt caactctctc tgcacctcca gtccaacgct gactgtctag
      721 tttacctact ggtctgatcg gctccatcag agctatggcg ttatcccgtg ccgttgctgc
      781 gcaatcgcta tcttgatcgc aaccttgaac tcactcttgt tttaatagtg atcttggtga
      841 cggagtgtcg gtgagtgaca accaacatcg tgcaagggag attgatacgg aagcttggcg
      901 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca cctcaatgcg
      961 gcaaactccg tgccaggcat tttaagctac cattctgtgg cgttatcata tgtgcctcga
     1021 ccggacggga cggcgttgtc ggcatcaccg gacgttttgg gcaaagcagg gaaatatgct
     1081 cgggtcccgt tcatcgtggg cgaccaagag gatgagggga ccttattcgc cttgtttcag
     1141 tccaacatta cgacgatcga cgaggtggtc gactacctgg cctcatactt cttctatgac
     1201 gctagccgag agcagcttga agaactagag gccctgtacc cagacaccac cacgtacgga
     1261 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1381 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1441 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1501 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1561 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1621 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1681 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1741 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1801 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1861 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1921 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1981 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2041 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2101 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2161 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2221 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2281 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2341 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     3361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     3421 attacgccaa gcttgcatgc aagcttcgta cgggtctccg ttcaggacag gcgcggccaa
     3481 caactggtat ccgcaattta agcgattggc cgccattctc ggcgacttgg tcttcaccat
     3541 tacccggcgg gcattcctct cgtatgcaga ggaaatctcc cctgatcttc cgaactggtc
     3601 gtacctggcg acctatgact atggcacccc agttctgggg accttccacg gaagtgacct
     3661 ggtgcaggtg ttctatggga tcaagccaaa ctatgcagct agttctagcc acacgtacta
     3721 tctgagcttt gtgtatacgc tggatccgaa ctccaaccag aattcgcggc cgcttctaga
     3781 g
//
