LOCUS       62056_3600        4279 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4..392)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(411..519)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(666..705)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(712..1569)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1570..1661)
                     /label=AmpR promoter
     CDS             complement(1718..3145)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    3239..3257
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             3365..4072
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    complement(4162..4218)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    complement(4250..4268)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc
       61 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaggctttaa
      121 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
      181 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
      241 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
      301 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
      361 tatcaacagg ttgaactgct gatcttcaga tcggcgcgcc ggccggccta cggccagcct
      421 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
      481 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
      541 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
      601 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaaggacaag
      661 ggtcgtccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggttt accaatgttt
      721 aatcaggctc gcgccaattt ccgcaatctg acggttacgt tcatccatcg tcgcctggct
      781 gccggtggta taaatcacca caatacggct cggtttacca tccgggccca gcgccgcaat
      841 aatgccacgg ctgccacgtt cgcccgcacc gcttttatcc gcaataaacc agccagccgg
      901 cagcgcgcta cgcagcagcg gacccgccac tttatccgct tccatccaat caatcagttg
      961 ctggcggctt gccagggtca gcagctcacc cgtcagcagt ttacgcaggg tggtcgccat
     1021 tgctgccggc atggtggtat cacgttcatc gttcggaatc gcttcgttca gttccggttc
     1081 ccaacgatcc agacgggtca catgatcgcc catgttatgc agaaacgcgg tcagttcttt
     1141 cggaccgcca atggtcgtca gcagcaggtt cgccgcggtg ttatcgctca tggtaatcgc
     1201 cgcgctgcac agttcacgca cggtcatgcc atcggtcaga tgtttttcgg tcaccgggct
     1261 atattccacc agatcgttct ggctataatg aatacgacgg cccagttgtt cctggcccgc
     1321 atccacacgg ctcagaaccg caccgcacag cagcacttta aaggtgctca tcatcggaaa
     1381 acgttcttcc ggacgaaaag attccagaat tttgccgctg ttcagatcca gttcaatata
     1441 gcccacgcgc gcacccagtt gatcttccgc atctttcact ttcaccaggg tttccggatg
     1501 cgcaaacacc ggcaggcaaa acgccgcaaa aaacggaatc agcgccacac gaaaatgctg
     1561 aatgctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggaat taaaaaaaag
     1681 cccgctcatt aggcgggcta ctaatttaaa tcgattagat tttaatgccc tgcgccatca
     1741 ggtctttcgc ggccagaaag ccatccagtt tgctttgcag cgcttcccaa ccttcccaca
     1801 gcgcacccca gctcgcaatg ccggtacgtt tgctatccat aaagccgccc agacgcgcaa
     1861 tcgccatata cgcccattgc aggctgcccg ctttttcttt gcgtttgcgt ttgcctttat
     1921 ccagatagcc cagcagttgg cattcatccg gggtcagcac ggtttccgcg ctctggcttt
     1981 caacgtgttc cgcttctttc agcaggccct gcgcacgcag tgcttgcggc ggagtaaaag
     2041 attcacgcag ttgcagcaga cgcaccgcca caaagctcag aatgctcacc atacgttcca
     2101 ggttatccgg ttcttccata cgctgacgtt ccgcacccgc acccgttttc cacgctttgt
     2161 gaaattcttc aatgcgccaa cgatgggtat aaatatcaat cacacgcagc gcttgggcca
     2221 gactttccac cggctcgctg gtcagcagca gccatttcag cggggtttcg cctttcggcg
     2281 gattaatttc ttcggccagc accgcgttca gggtaatgtt gccctgtttc agggtaatac
     2341 ggccgctacg caggctcagg ctcgctttac gcgccggacg gtttttacgt ttgccacgtt
     2401 tatccaccac gcctttctgc ggaatgctaa tctgatagcc gcccagttcc ggctggtttt
     2461 tcaggtgatc atacagatac aggccgcttt ccacatcttt acgcggatgt ttgctacgca
     2521 ccacaaaacg ttcgttatgg gccagtttat cttgcagata cgcatgaata tccgcttcac
     2581 gatcgcacac cgcaatcacg ttgctcatca tgctgcccat tctcagacgc gaagttgcag
     2641 cagcggccag ccatttgccg ctttcttttt catccgcatc cgccggatca tccggacgca
     2701 tccaccattc ttgatgcagc aggcccacgg tacgaaaggt ggtcgcttcc agcagcagca
     2761 cgctatgcac ccaccaacca cggcttttat cctgaatgct acccagtttg cccagttctt
     2821 ccgccacctg atgacgatag ctcagagagg tggtatcttc aattgccagc agttccggaa
     2881 attcctgggc cagtttcacg gtctgcatgg cacccgcttt acgaatcgct tccgcgctca
     2941 cgttcggatt acgaataaaa cgatacgcgc cttcctgcat ggctttgctg ccttcgctgc
     3001 taatggtaat gcttttgccg ctatatttgg ccagttgcgc cgcaacattc accagacgcg
     3061 cggtacgacg cggatcaccc agcgcagcac tagaaaacac gcttttcgcc caatccgccg
     3121 cacgatgcag tgcactggta atcatgaacg ttaccatgtt aggaggtcac atggaacatc
     3181 agattctgga aaacgggaaa ggttccgttc aggacgctac ttgtgtagtt taaaccagct
     3241 gtctcttata cacatctgac gtctcgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3301 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     3361 tgttatggtt tctaaaggtg aagaagacaa catggctatc atcaaagaat tcatgcgttt
     3421 caaagttcac atggaaggtt ctgttaacgg tcacgaattc gaaatcgaag gtgaaggtga
     3481 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
     3541 gccgttcgct tgggacatcc tgtctccgca gttcatgtac ggttctaaag cttacgttaa
     3601 acacccggct gacatcccgg actacctgaa actgtctttc ccggaaggtt tcaaatggga
     3661 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cttctctgca
     3721 ggacggtgaa ttcatctaca aagttaaact gcgtggtacc aacttcccgt ctgacggtcc
     3781 ggttatgcag aaaaaaacca tgggttggga agcttcttct gaacgtatgt acccggaaga
     3841 cggtgctctg aaaggtgaaa tcaaacagcg tctgaaactg aaagacggtg gtcactacga
     3901 cgctgaagtt aaaaccacct acaaagctaa aaaaccggtt cagctgccgg gtgcttacaa
     3961 cgttaacatc aaactggaca tcacctctca caacgaagac tacaccatcg ttgaacagta
     4021 cgaacgtgct gaaggtcgtc actctaccgg tggtatggac gaactgtaca aataataatt
     4081 aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcacaa aacggtttac
     4141 aagcataact agtgcggccg caagcttgca tgcctgcagg tcgactctag aggatccccg
     4201 ggtaccgagc tcgaattcgc gcggccgcgg cctaggcggc cttaattaaa gatgtgtata
     4261 agagacagct gggcgcgcc
//
