LOCUS       V015718                 5823 bp    DNA     circular SYN 12-JUN-2021
DEFINITION  Exported.
ACCESSION   V015718
VERSION     V015718
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Robertson WE, Funke LFH., de la Torre D, Fredens J, Elliott TS,
            Spinck M, Christova Y, Cervettini D, Boge FL, Liu KC, Buse S, Maslen
            S, Salmond GPC., Chin JW.
  TITLE     Sense Codon Reassignment Enables Viral Resistance and Encoded
            Polymer Synthesis
  JOURNAL   Science (2021) In press
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Robertson WE, Funke LFH., de la Torre D, Fredens J, Elliott TS,
            Spinck M, Christova Y, Cervettini D, Boge FL, Liu KC, Buse S, Maslen
            S, Salmond GPC., Chin JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2021) Protein and Nucleic Acid Chemistry, MRC
            Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge,
            Cambridgeshire CB2 0QH, United Kingdom
REFERENCE   3  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science
            (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2021) Protein and Nucleic Acid Chemistry, MRC Laboratory of
            Molecular Biology, Francis Crick Avenue, Cambridge, Cambridgeshire
            CB2 0QH, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..5823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            41..65
                     /gene="glnS"
                     /label="glnS"
     regulatory      41..65
                     /gene="glnS"
                     /note="GlnS' promoter; GlnS' p"
                     /regulatory_class="promoter"
     CDS             107..1024
                     /gene="tyrS"
                     /label="Tyrosine--tRNA ligase"
                     /note="Tyrosine--tRNA ligase from Methanocaldococcus
                     jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
                     10045 / NBRC 100440). Accession#: Q57834"
     regulatory      1550..1600
                     /label="lpp promoter"
                     /note="lpp promoter"
                     /regulatory_class="promoter"
     tRNA            1607..1683
                     /product="tRNA-Tyr"
                     /label="Mj tRNA Tyr(CUA)"
                     /note="Mj tRNA Tyr(CUA)"
     regulatory      1690..1718
                     /note="rrnC terminator; rrnC term"
                     /regulatory_class="terminator"
     terminator      1865..1951
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2292..2837)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        2949..2977
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        3025..3116
                     /label="AmpR promoter"
     CDS             3117..3917
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(4231..5106)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        5133..5417
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             5679..5696
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             5722..5751
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             5767..5784
                     /label="6xHis"
                     /note="6xHis affinity tag"
ORIGIN
        1 gagagaagat tttcagcctg atacagatta aatcagaacg ttgtcagcct gtcccgcttt
       61 aatatcatac gccgttatac gttgtttacg ctttgaggaa tcccatatgg acgaatttga
      121 aatgataaag agaaacacat ctgaaattat cagcgaggaa gagttaagag aggttttaaa
      181 aaaagatgaa aaatctgctc atataggttt tgaaccaagt ggtaaaatac atttagggca
      241 ttatctccaa ataaaaaaga tgattgattt acaaaatgct ggatttgata taattatatt
      301 gttggctgat ttaaccgcct atttaaacca gaaaggagag ttggatgaga ttagaaaaat
      361 aggagattat aacaaaaaag tttttgaagc aatggggtta aaggcaaaat atgtttatgg
      421 aagtgaattc cagcttgata aggattatac actgaatgtc tatagattgg ctttaaaaac
      481 taccttaaaa agagcaagaa ggagtatgga acttatagca agagaggatg aaaatccaaa
      541 ggttgctgaa gttatctatc caataatgca ggttaatcat gcgcattatc gtggcgttga
      601 tgttgcagtt ggagggatgg agcagagaaa aatacacatg ttagcaaggg agcttttacc
      661 aaaaaaggtt gtttgtattc acaaccctgt cttaacgggt ttggatggag aaggaaagat
      721 gagttcttct aaagggaatt ttatagctgt tgatgactct ccagaagaga ttagggctaa
      781 gataaagaaa gcatactgcc cagctggagt tgttgaagga aatccaataa tggagatagc
      841 taaatacttc cttgaatatc ctttaaccat aaaaaggcca gaaaaatttg gtggagattt
      901 gacagttaat agctatgagg agttagagag tttatttaaa aataaggaat tgcatccaat
      961 ggatttaaaa aatgctgtag ctgaagaact tataaagatt ttagagccaa ttagaaagag
     1021 attataactg cagtttcaaa cgctaaattg cctgatgcgc tacgcttatc aggcctacat
     1081 gatctctgca atatattgag tttgcgtgct tttgtaggcc ggataaggcg ttcacgccgc
     1141 atccggcaag aaacagcaaa caatccaaaa cgccgcgttc ggcggtcgac acagatgtag
     1201 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     1261 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgctg
     1321 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     1381 tcatgccccg cgcccaccgg aaggagctac cggcagcggt gcggactgtt gtaactcaga
     1441 ataagaaatg aggccgctca tggcgttctg ttgcccgtct cactggtgaa aagaaaaaca
     1501 accctggcgc cgcttctttg agcgaacgat caaaaataag tggcgcccca tcaaaaaaat
     1561 attctcaaca taaaaaactt tgtgtaatac ttgtaacgct agatctccgg cggtagttca
     1621 gcagggcaga acggcggact ctaaatccgc atggcagggg ttcaaatccc ctccgccgga
     1681 ccaactagta tccttagcga aagctaagga ttttttttca gaagcggtct gataaaacag
     1741 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     1801 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
     1861 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1921 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gctgtccctc ctgttcagct
     1981 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
     2041 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
     2101 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
     2161 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     2221 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     2281 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     2341 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     2401 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     2461 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     2521 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     2581 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     2641 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     2701 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     2761 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     2821 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     2881 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat
     2941 tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc acagttaaat
     3001 tgctaacgca gtcaggcacc gtgttttgtt tatttttcta aatacattca aatatgtatc
     3061 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3121 gtagtgcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     3181 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     3241 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctgcgctgg
     3301 gtccgggagg gacgctcgtc atgcccagct ggagtggtct ggacgacgag ccgttcgatc
     3361 ctgccacgag ccccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     3421 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     3481 agatcatctc tgatccattg cccctgccac ctcacagccc tgcaagcccg gtcgcccgtg
     3541 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     3601 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     3661 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     3721 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     3781 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     3841 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     3901 ctcgccagag cattggctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     3961 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
     4021 gaacatatcc atcgcgtgcg gccgcgtctc cgggagctgc atgtgtcaga ggttttcacc
     4081 gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc
     4141 ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa
     4201 gccgtcaatt gtctgattcg ttaccaatta actcaacttg acggctacat cattcacttt
     4261 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
     4321 gctgaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
     4381 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
     4441 gctaatccct aactgctggc ggaaaagatg actcagacgg ctcgggctca agcaaacatg
     4501 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
     4561 agcctcgcgt acccgattat ccatcggtgg atggaggctc tcgttaatcg cttccatgcg
     4621 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tcgctatagc gcccttcccc
     4681 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
     4741 cgggcgaaag aaccccgtat tggcaaatat actcggccag ttaagccatt catgccagta
     4801 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc
     4861 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc
     4921 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt
     4981 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
     5041 acccgccacc agatgggcat taaagctgta tcccggcagc aggggatcat tttgcgcttc
     5101 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
     5161 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
     5221 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
     5281 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
     5341 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
     5401 ctctactgtt tctccatacc cgttttttgg gctaacagga ggaattaacc atgcagatct
     5461 ttgttaaaac cctgtagggc tcgaccattt cactggaagt tgaaccgagc gataccattg
     5521 aaaatgtgaa agccaaaatt caggataaag aaggtattcc gcctgatcag cagcgtctga
     5581 tttttgcagg taaacagctg gaagatggtc gtaccctgag cgattataac attcagaaag
     5641 aaagcaccct gcatctggtt ctgcgtctgc gtggtggcca ccatcatcat caccattaaa
     5701 gctcgagcga agcttgggcc cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc
     5761 gtcgaccatc atcatcatca tcattgagtt taaacggtct ccagcttggc tgttttggcg
     5821 gat
//
