LOCUS       Exported                6187 bp DNA     circular SYN 06-DEC-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6187)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(194..1069)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1096..1380
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     terminator      1664..1750
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1842..1869
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     complement(1953..1969)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2495..3283)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKEVAADWAMERLPAQYQP
                     VIREARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4323..5270)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(5318..5540)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     protein_bind    6107..6128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6143..6173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cctgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
       61 ggcgtagagg atctgctcat gtttgacagc ttatcatcga tgcataatgt gcctgtcaaa
      121 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
      181 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
      241 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
      301 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      361 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      421 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      481 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      541 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      601 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      661 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      721 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      781 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      841 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      901 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      961 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     1021 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     1081 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     1141 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1201 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1261 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1321 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1381 acccgttttt ttgggctagc aggaggaatt caccatggta cccggggatc ctctagagtc
     1441 gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt cagcctgata
     1501 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
     1561 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
     1621 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     1681 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     1741 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
     1801 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
     1861 tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt
     1921 atccgctcat gagacaataa ccctagcttg gcactggccg tcgttttaca acgtcgtgac
     1981 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2041 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2101 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2161 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     2221 ccgccaacac ccgctgacga gcttagtaaa gccctcgcta gattttaatg cggatgttgc
     2281 gattacttcg ccaactattg cgataacaag aaaaagccag cctttcatga tatatctccc
     2341 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     2401 tttggctgtg agcaattatg tgcttagtgc atctaacgct tgagttaagc cgcgccgcga
     2461 agcggcgtcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
     2521 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     2581 cttgtccaag ataagcctgt ctagcttcac gtatgacggg ctgatactgg gccggcaggc
     2641 gctccattgc ccagtcggca gcgacctcct tcggcgcgat tttgccggtt actgcgctgt
     2701 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     2761 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     2821 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     2881 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     2941 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     3001 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     3061 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     3121 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     3181 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     3241 ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt aactttgttt
     3301 tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca
     3361 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt
     3421 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     3481 tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt
     3541 atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     3601 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     3661 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     3721 atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc
     3781 tgaccccgga tgaagtaatt cccacgggtt ttgctgcccg caaacgggct gttctggtgt
     3841 tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc tgaaagcgct
     3901 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     3961 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     4021 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     4081 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     4141 ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     4201 caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg tgaacagttg
     4261 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     4321 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     4381 catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa ttttgcctca
     4441 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     4501 tgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     4561 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     4621 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     4681 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     4741 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     4801 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     4861 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     4921 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     4981 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     5041 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     5101 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     5161 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     5221 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     5281 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     5341 gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt
     5401 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     5461 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     5521 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     5581 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     5641 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     5701 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     5761 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     5821 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     5881 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     5941 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     6001 gcttacccgt cttactgtcg ggaattaatt cgcgttggcc gattcattaa tgcagctggc
     6061 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6181 ttgtgag
//
