LOCUS       .                       6774 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     misc_feature    1306..1362
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      1565..1651
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1743..1770
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1789..1880
                     /label="AmpR promoter"
     promoter        2130..2234
                     /label="AmpR promoter"
     CDS             2309..3496
                     /label="TcR"
                     /note="tetracycline efflux protein"
     rep_origin      3756..4211
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(4769..5425)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5426..5528)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(6054..6599)
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt
     1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
     1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
     1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
     1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1981 ctgaagatca gttggggcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2041 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2101 ccggcggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2221 gaaaaaggaa gagtatgagt attcaaccga ttagctttaa tgcggtagtt tatcacagtt
     2281 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     2341 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     2401 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     2461 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
     2521 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
     2581 gaccacaccc gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc
     2641 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     2701 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     2761 gggactgttg ggcgccatct ccttacatgc accattcctt gcggcggcgg tgctcaacgg
     2821 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgacc
     2881 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     2941 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     3001 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     3061 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     3121 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     3181 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     3241 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     3301 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     3361 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     3421 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     3481 ccgggccacc tcgacctgac tgtcagacca agtttactca tatatactgg ctggtttatt
     3541 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3601 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3661 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3721 gaccaagttt actcatatat actttagatt gatttacgcg ccctgtagcg gcgcattaag
     3781 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3841 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3901 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3961 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4021 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa cttgaacaac
     4081 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     4141 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4201 gtttacaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4441 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtcaggc
     4561 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     4621 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     4681 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag
     4741 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     4801 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
     4861 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     4921 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     4981 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     5041 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     5101 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     5161 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     5221 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     5281 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     5341 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     5401 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     5461 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     5521 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     5581 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     5641 gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     5701 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
     5761 aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg
     5821 gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt
     5881 gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg
     5941 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
     6001 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     6061 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     6121 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
     6181 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     6241 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     6301 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     6361 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     6421 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     6481 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     6541 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     6601 aagatcatct tattaatcag ataaaatatt tgctcatgag cccgaagtgg cgagcccgat
     6661 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     6721 tgccggccac gatgcgtccg gcgtagagga tctgctcatg tttgacagct tatc
//
