LOCUS       40924_5849        5535 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBAD322S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Cronan JE.
  TITLE     A family of arabinose-inducible Escherichia coli expression vectors 
            having pBR322 copy control
  JOURNAL   Plasmid 55 (2), 152-157 (2006)
  PUBMED    16139359
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Cronan JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2005) Microbiology, University of Illinois, 601 S.
            Goodwin Ave., Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 5535)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2006"; volume: "55"; issue: "2"; pages: "152-157"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2005) Microbiology, University of Illinois, 601 S. Goodwin 
            Ave., Urbana, IL 61801, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     terminator      1569..1655
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1747..1774
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(2216..3004)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3933..4521
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4707..4847)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4952..5140)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg
     1381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     1441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     1501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1621 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     1681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     1741 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     1801 tttttctaaa tacattcaaa tatgtatccg ctcatggagc tcgatgcttg taaaccgttt
     1861 tgtgaaaaaa tttttaaaat aaaaaagggg acctctaggg tccccaatta attagtaata
     1921 taatctatta aaggtcattc aaaaggtcat ccaccggatc agcttagtaa agccctcgct
     1981 agattttaat gcggatgttg cgattacttc gccaactatt gcgataacaa gaaaaagcca
     2041 gcctttcatg atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata
     2101 aaagcagact tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaacgc
     2161 ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg aattgttaga cattatttgc
     2221 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc
     2281 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg
     2341 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
     2401 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
     2461 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
     2521 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
     2581 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
     2641 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
     2701 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
     2761 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
     2821 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca
     2881 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
     2941 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
     3001 tcatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc
     3061 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     3121 actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     3181 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     3241 gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg
     3301 tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga
     3361 acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct
     3421 acggcaaggt ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc
     3481 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga
     3541 aggcgagcat cgtttgttcg cccagcttct gtatggaacg ggcatgcgga tcagtgaggg
     3601 tttgcaactg cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg
     3661 caagggctcc aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga
     3721 gcaggggaat tgatccggtg gatgaccttt tgaatgacct ttaatagatt atattactaa
     3781 ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac aaaacggttt
     3841 acaagcatcg agctctccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3901 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3961 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4021 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     4081 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4141 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4201 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4261 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4321 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4381 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4441 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4501 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4561 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4621 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4681 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     4741 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4801 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     4861 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4921 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4981 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
     5041 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     5101 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     5161 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     5221 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     5281 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgctag cagcacgcca
     5341 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
     5401 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
     5461 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
     5521 cattaaagct agctt
//
