LOCUS       V009295                 4619 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009295
VERSION     V009295
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     MipZ, a spatial regulator coordinating chromosome segregation with
            cell division in Caulobacter
  JOURNAL   Cell 126 (1), 147-162 (2006)
   PUBMED   16839883
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2005) Developmental Biology, Stanford University,
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2006) Developmental Biology, Stanford University,
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   4  (bases 1 to 4619)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4619)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
            date: "2006"; volume: "126"; issue: "1"; pages: "147-162"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-OCT-2005) Developmental Biology, Stanford University, 279 Campus
            Drive, Stanford, CA 94305, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (18-JAN-2006) Developmental Biology, Stanford University, 279 Campus
            Drive, Stanford, CA 94305, USA"
            SGRef: number: 4; type: "Journal Article"
            On Jan 18, 2006 this sequence version replaced DQ266354.1.
FEATURES             Location/Qualifiers
     source          1..4619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     regulatory      1306..1312
                     /regulatory_class="ribosome_binding_site"
     terminator      1569..1655
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1747..1774
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1793..1884
                     /label="AmpR promoter"
     CDS             2058..3053
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     rep_origin      3430..4018
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg
     1381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     1441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     1501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1621 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     1681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     1741 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     1801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1981 gatgctgaag atcagttggg tgcacgaccg gccgtgcgga attaagccgg cccgtaccct
     2041 gtgaatagag gtccgctgtg acacaagaat ccctgttact tctcgaccgt attgattcgg
     2101 atgattccta cgcgagcctg cggaacgacc aggaattctg ggagccgctg gcccgccgag
     2161 ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc gagagcacca
     2221 accccgtact ggtcggcgag cccgacccgg tgatcaagct gttcggcgag cactggtgcg
     2281 gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac gccccggtgc
     2341 cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc tggccgtggc
     2401 cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg gacggcacga
     2461 ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc ggccggctgc
     2521 acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc ttcccggaac
     2581 tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc tacctctcgc
     2641 cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg ctggccggcc
     2701 gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg gacctggccg
     2761 cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac tcccgctaca
     2821 gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg gccgcgctgc
     2881 tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc gccttcacct
     2941 tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc ttcaccgatc
     3001 cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc gcctgacgcc
     3061 ccgggccgcc cggcgccgcc cccggccccc ggcggccgtg ggccatcgcc ctgatagacg
     3121 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3181 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     3241 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3301 atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3361 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3421 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3481 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3541 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3601 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3661 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3721 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3781 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3841 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3901 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3961 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4021 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4081 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4141 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4201 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggtc
     4261 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4321 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4381 tcaccgtcat caccgaaacg cgcgaggcag caaggagatg gcgcccaaca gtcccccggc
     4441 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     4501 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     4561 ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatc
//
