LOCUS       40924_5799        5013 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAD24-Gluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Wille T, Blank K, Schmidt C, Vogt V, Gerlach RG.
  TITLE     Gaussia princeps Luciferase as a Reporter for Transcriptional 
            Activity, Protein Secretion, and Protein-Protein Interactions in 
            Salmonella enterica Serovar Typhimurium
  JOURNAL   Appl. Environ. Microbiol. 78 (1), 250-257 (2012)
  PUBMED    22020521
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Wille T, Blank K, Schmidt C, Gerlach RG.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2010) Junior Research Group 3, Robert 
            Koch-Institute, Burgstrasse 37, Wernigerode, Saxony-Anhalt 38855, 
            Germany
REFERENCE   3  (bases 1 to 5013)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5013)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "1"; pages:
            "250-257"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2010) Junior Research Group 3, Robert Koch-Institute, 
            Burgstrasse 37, Wernigerode, Saxony-Anhalt 38855, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1306..1311
                     /regulatory_class="ribosome_binding_site"
     CDS             1319..1828
                     /codon_start=1
                     /gene="Gluc"
                     /product="Gluc"
                     /label=Gluc
                     /note="luciferase; derived from Gaussia princeps; codon 
                     optimized for Salmonella enterica expression"
                     /protein_id="AEA02464.1"
                     /translation="MKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEME
                     ANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPG
                     FKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKI
                     KGAGGD"
     gene            1319..1828
                     /gene="Gluc"
                     /label=Gluc
     terminator      2040..2126
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2218..2245
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2264..2355
                     /label=AmpR promoter
     CDS             2356..3213
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3258..3713
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      3824..4412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattctaat
     1321 gaaaccgacc gaaaacaacg aagattttaa cattgtggcg gtggcgagca actttgcgac
     1381 caccgatctg gatgcggatc gtggcaaact gccgggcaaa aaactgccgc tggaagtgct
     1441 gaaagaaatg gaagcgaatg cgcgtaaagc gggttgcacc cgtggctgcc tgatttgcct
     1501 gagccatatt aaatgcaccc cgaaaatgaa aaaatttatt ccgggtcgtt gccataccta
     1561 tgaaggcgat aaagaaagcg cgcagggtgg cattggcgaa gcgattgtgg atattccgga
     1621 aattccgggc tttaaagatc tggaaccgat ggaacagttt attgcgcagg tcgatctgtg
     1681 cgtggattgc accaccggct gcctgaaagg cctggcgaac gtgcagtgca gcgatctgct
     1741 gaaaaaatgg ctgccgcagc gctgtgcgac ctttgcgagc aaaattcagg gccaggtgga
     1801 taaaattaaa ggtgcgggtg gcgattaata aaagcttggc tgttttggcg gatgagagaa
     1861 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
     1921 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
     1981 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
     2041 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2101 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
     2161 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
     2221 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa
     2281 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2341 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2401 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2461 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2521 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2581 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     2641 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2701 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2761 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2821 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2881 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2941 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3001 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3061 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3121 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3181 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3241 atactttaga ttgatttacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3301 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3361 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3421 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3481 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3541 cacgttcttt aatagtggac tcttgttcca aacttgaaca acactcaacc ctatctcggg
     3601 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3661 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttaaaagga
     3721 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3781 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3841 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3901 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3961 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4021 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4081 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4141 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4201 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4261 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4321 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4381 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4441 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4501 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4561 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     4621 cgcatctgtg cggtatttca caccgcatag ggtcatggct gcgccccgac acccgccaac
     4681 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4741 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4801 gcagcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     4861 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     4921 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     4981 cgtagaggat ctgctcatgt ttgacagctt atc
//
