LOCUS       V009301                 9120 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009301
VERSION     V009301
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Hansen DT, Thiyagarajan T, Larson AC, Hansen JL.
  TITLE     Telomerase repeat amplification protocol (TRAP) activity upon
            recombinant expression and purification of human telomerase in a
            bacterial system
  JOURNAL   Protein Expr. Purif. (2016) In press
   PUBMED   26965413
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Hansen DT, Hansen JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2015) Department of Biochemistry and Molecular
            Biology, Medical University of South Carolina, 173 Ashley Avenue,
            Charleston, SC 29425, USA
REFERENCE   3  (bases 1 to 9120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein
            Expr. Purif. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-DEC-2015) Department of Biochemistry and Molecular Biology,
            Medical University of South Carolina, 173 Ashley Avenue, Charleston,
            SC 29425, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     RBS             1312..1334
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1353..1370
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1389..4784
                     /label="hTERT"
                     /note="human telomerase reverse transcriptase, the
                     catalytic subunit of telomerase"
     CDS             4868..5851
                     /gene="PINX1"
                     /label="PIN2/TERF1-interacting telomerase inhibitor 1"
                     /note="PIN2/TERF1-interacting telomerase inhibitor 1 from
                     Homo sapiens. Accession#: Q96BK5"
     terminator      6072..6158
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6250..6277
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        6296..6387
                     /label="AmpR promoter"
     CDS             6388..7245
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7290..7745
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      7856..8444
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8630..8770)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
     1321 ctttaagaag gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc
     1381 cgaattctat gccgcgcgct ccccgctgcc gagccgtgcg ctccctgctg cgcagccact
     1441 accgcgaggt gctgccgctg gccacgttcg tgcggcgcct ggggccccag ggctggcggc
     1501 tggtgcagcg cggggacccg gcggctttcc gcgcgctggt ggcccagtgc ctggtgtgcg
     1561 tgccctggga cgcacggccg ccccccgccg ccccctcctt ccgccaggtg tcctgcctga
     1621 aggagctggt ggcccgagtg ctgcagaggc tgtgcgagcg cggcgcgaag aacgtgctgg
     1681 ccttcggctt cgcgctgctg gacggggccc gcgggggccc ccccgaggcc ttcaccacca
     1741 gcgtgcgcag ctacctgccc aacacggtga ccgacgcact gcgggggagc ggggcgtggg
     1801 ggctgctgct gcgccgcgtg ggcgacgacg tgctggttca cctgctggca cgctgcgcgc
     1861 tctttgtgct ggtggctccc agctgcgcct accaggtgtg cgggccgccg ctgtaccagc
     1921 tcggcgctgc cactcaggcc cggcccccgc cacacgctag tggaccccga aggcgtctgg
     1981 gatgcgaacg ggcctggaac catagcgtca gggaggccgg ggtccccctg ggcctgccag
     2041 ccccgggtgc gaggaggcgc gggggcagtg ccagccgaag tctgccgttg cccaagaggc
     2101 ccaggcgtgg cgctgcccct gagccggagc ggacgcccgt tgggcagggg tcctgggccc
     2161 acccgggcag gacgcgtgga ccgagtgacc gtggtttctg tgtggtgtca cctgccagac
     2221 ccgccgaaga agccacctct ttggagggtg cgctctctgg cacgcgccac tcccacccat
     2281 ccgtgggccg ccagcaccac gcgggccccc catccacatc gcggccacca cgtccctggg
     2341 acacgccttg tcccccggtg tacgccgaga ccaagcactt cctctactcc tcaggcgaca
     2401 aggagcagct gcggccctcc ttcctactca gctctctgag gcccagcctg actggcgctc
     2461 ggaggctcgt ggagaccatc tttctgggtt ccaggccctg gatgccaggg actccccgca
     2521 ggttgccccg cctgccccag cgctactggc aaatgcggcc cctgtttctg gagctgcttg
     2581 ggaaccacgc gcagtgcccc tacggggtgc tcctcaagac gcactgcccg ctgcgagctg
     2641 cggtcacccc agcagccggt gtctgtgccc gggagaagcc ccagggctct gtggcggccc
     2701 ccgaggagga ggacacagac ccccgtcgcc tggtgcagct gctccgccag cacagcagcc
     2761 cctggcaggt gtacggcttc gtgcgggcct gcctgcgccg gctggtgccc ccaggcctct
     2821 ggggctccag gcacaacgaa cgccgcttcc tcaggaacac caagaagttc atctccctgg
     2881 ggaagcatgc caagctctcg ctgcaggagc tgacgtggaa gatgagcgtg cgggactgcg
     2941 cttggctgcg caggagccca ggggttggct gtgttccggc cgcagagcac cgtctgcgtg
     3001 aggagatcct ggccaagttc ctgcactggc tgatgagtgt gtacgtcgtc gagctgctca
     3061 ggtctttctt ttatgtcacg gagaccacgt ttcaaaagaa caggctcttt ttctaccgga
     3121 agagtgtctg gagcaagttg caaagcattg gaatcagaca gcacttgaag agggtgcagc
     3181 tgcgggagct gtcggaagca gaggtcaggc agcatcggga agccaggccc gccctgctga
     3241 cgtccagact ccgcttcatc cccaagcctg acgggctgcg gccgattgtg aacatggact
     3301 acgtcgtggg agccagaacg ttccgcagag aaaagagggc cgagcgtctc acctcgaggg
     3361 tgaaggcact gttcagcgtg ctcaactacg agcgggcgcg gcgccccggc ctcctgggcg
     3421 cctctgtgct gggcctggac gatatccaca gggcctggcg caccttcgtg ctgcgtgtgc
     3481 gggcccagga cccgccgcct gagctgtact ttgtcaaggt ggatgtgacg ggcgcgtacg
     3541 acaccatccc ccaggacagg ctcacggagg tcatcgccag catcatcaaa ccccagaaca
     3601 cgtactgcgt gcgtcggtat gccgtggtcc agaaggccgc ccatgggcac gtccgcaagg
     3661 ccttcaagag ccacgtctct accttgacag acctccagcc gtacatgcga cagttcgtgg
     3721 ctcacctgca ggagaccagc ccgctgaggg atgccgtcgt catcgagcag agctcctccc
     3781 tgaatgaggc cagcagtggc ctcttcgacg tcttcctacg cttcatgtgc caccacgccg
     3841 tgcgcatcag gggcaagtcc tacgtccagt gccaggggat cccgcagggc tccatcctct
     3901 ccacgctgct ctgcagcctg tgctacggcg acatggagaa caagctgttt gcggggattc
     3961 ggcgggacgg gctgctcctg cgtttggtgg atgatttctt gttggtgaca cctcacctca
     4021 cccacgcgaa aaccttcctc aggaccctgg tccgaggtgt ccctgagtat ggctgcgtgg
     4081 tgaacttgcg gaagacagtg gtgaacttcc ctgtagaaga cgaggccctg ggtggcacgg
     4141 cttttgttca gatgccggcc cacggcctat tcccctggtg cggcctgctg ctggataccc
     4201 ggaccctgga ggtgcagagc gactactcca gctatgcccg gacctccatc agagccagtc
     4261 tcaccttcaa ccgcggcttc aaggctggga ggaacatgcg tcgcaaactc tttggggtct
     4321 tgcggctgaa gtgtcacagc ctgtttctgg atttgcaggt gaacagcctc cagacggtgt
     4381 gcaccaacat ctacaagatc ctcctgctgc aggcgtacag gtttcacgca tgtgtgctgc
     4441 agctcccatt tcatcagcaa gtttggaaga accccacatt tttcctgcgc gtcatctctg
     4501 acacggcctc cctctgctac tccatcctga aagccaagaa cgcagggatg tcgctggggg
     4561 ccaagggcgc cgccggccct ctgccctccg aggccgtgca gtggctgtgc caccaagcat
     4621 tcctgctcaa gctgactcga caccgtgtca cctacgtgcc actcctgggg tcactcagga
     4681 cagcccagac gcagctgagt cggaagctcc cggggacgac gctgactgcc ctggaggccg
     4741 cagccaaccc ggcactgccc tcagacttca agaccatcct ggactgagcg gccgcataat
     4801 gcttaagtcg aacagaaagt aatcgtattg tacccgggga tcctctagat aagaaggaga
     4861 tatacatatg tctatgctgg ctgaacgtcg gcggaagcag aagtgggctg tggatcctca
     4921 gaacactgcc tggagtaatg acgattccaa gtttggccag cggatgctag agaagatggg
     4981 gtggtctaaa ggaaagggtt taggggctca ggagcaagga gccacagatc atattaaagt
     5041 tcaagtgaaa aataaccacc tgggactcgg agctaccatc aataatgaag acaactggat
     5101 tgcccatcag gatgatttta accagcttct ggccgaactg aacacttgcc atgggcagga
     5161 aaccacagat tcctcggaca agaaggaaaa gaaatctttt agccttgagg aaaagtccaa
     5221 aatctccaaa aaccgtgttc actatatgaa attcacaaaa gggaaggatc tgtcatctcg
     5281 gagcaaaaca gatcttgact gcatttttgg gaaaagacag agtaagaaga ctcccgaggg
     5341 cgatgccagt ccctccactc cagaggagaa cgaaaccacg acaaccagcg ccttcaccat
     5401 ccaggagtac tttgccaagc ggatggcagc actgaagaac aagccccagg ttccagttcc
     5461 agggtctgac atttctgaga cgcaggtgga acgtaaaagg gggaagaaaa gaaataaaga
     5521 ggccacaggt aaagatgtgg aaagttacct ccagcctaag gccaagaggc acacggaggg
     5581 aaagcccgag agggccgagg cccaggagcg agtggccaag aagaagagcg cgccagcaga
     5641 agagcagctc agaggcccct gctgggacca gagttccaag gcctctgctc aggatgcagg
     5701 ggaccatgtg cagccgcctg agggccggga cttcaccctg aagcccaaaa agaggagagg
     5761 gaagaaaaag ctgcaaaaac cagtagagat agcagaggac gctacactag aagaaacgct
     5821 agtgaaaaag aagaagaaga aagattccaa ataataaggt accaagcttg gctgttttgg
     5881 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     5941 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     6001 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     6061 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     6121 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     6181 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     6241 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     6301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6601 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6901 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     6961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7261 gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc
     7321 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7381 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7441 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7501 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7561 gacgttggag tccacgttct ttaatagtgg actcttgttc caaacttgaa caacactcaa
     7621 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7681 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     7741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8221 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     8641 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     8701 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8761 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8821 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8881 ttcaccgtca tcaccgaaac gcgcgaggca gcaaggagat ggcgcccaac agtcccccgg
     8941 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     9001 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     9061 cggtgatgcc ggccacgatg cgtccggcgt agaggatctg ctcatgtttg acagcttatc
//
