LOCUS       62056_3510        4126 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             312..334
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             364..378
                     /codon_start=1
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
                     /translation="DDDDK"
     CDS             403..444
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             454..471
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      697..783
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      875..902
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        921..1012
                     /label=AmpR promoter
     CDS             1013..1870
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2044..2632
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2818..2958)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3225..4100)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg ctctggatcc
      361 ggtgatgacg atgacaagct cgcccttaag ggcgagcttg aaggtaagcc tatccctaac
      421 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
      481 acggtctcca gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
      541 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
      601 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
      661 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
      721 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
      781 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
      841 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
      901 tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct
      961 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1021 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1081 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1141 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1201 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     1261 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1321 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1381 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1441 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1501 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1561 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1621 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1681 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1741 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1801 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1861 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1921 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1981 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2041 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2101 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2161 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     2221 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2281 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2341 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2401 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2461 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2521 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2581 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2641 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2701 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2761 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     2821 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     2881 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     2941 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3001 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3061 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc gcgcgcgaag
     3121 gcgaagcggc atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta
     3181 tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct
     3241 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
     3301 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
     3361 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
     3421 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
     3481 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
     3541 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
     3601 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
     3661 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
     3721 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc
     3781 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
     3841 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
     3901 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
     3961 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
     4021 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga
     4081 tcattttgcg cttcagccat acttttcata ctcccgccat tcagag
//
