LOCUS       62056_3490        5945 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(264..794)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(983..1011)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(1354..2229)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2256..2540
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(2605..2621)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3494..4153)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(4154..4923)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
      241 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
      301 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
      361 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
      421 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
      481 tcactacgcg gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct
      541 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
      601 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
      661 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
      721 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      781 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      841 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      901 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
      961 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1021 tgccttcatc cgtttccacg gtgtgcgtcc atgggcaaat attatacgca aggcgacaag
     1081 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     1141 agaatgctta atgaattaca acagttttta tgcatgcgcc caatacgcaa accgcctctc
     1201 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1261 ggcagtgtgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
     1321 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac
     1381 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
     1441 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
     1501 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
     1561 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
     1621 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
     1681 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
     1741 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
     1801 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
     1861 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
     1921 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
     1981 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
     2041 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
     2101 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
     2161 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
     2221 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
     2281 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc
     2341 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
     2401 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
     2461 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
     2521 actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta accaagcttg
     2581 atatcgaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg
     2641 agctctaata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
     2701 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     2761 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
     2821 aacgaaaggc tcagtcgaaa gactgggcct ttcgtcgacg cgctagcgga gtgtatactg
     2881 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     2941 aggctgcacc ggtgcgtcag cagaatatgt aggccgactg cgatgagtgg cagggcaggc
     3001 cgactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
     3061 aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa
     3121 gcaaattcga cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt
     3181 gctggtttac cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct
     3241 gaggccagtt tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt
     3301 ctgcctccca gagcctgata aaaacggtga atccgttagc gaggtgccgc cggcttccat
     3361 tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
     3421 tagggcggcg aggcggctac agccgatagt ctggaacagc gcacttacgg gttgctgcgc
     3481 aacccaagtg ctaccggcgc ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat
     3541 cgtccagaaa acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt
     3601 cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg
     3661 gcggctcctc gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc
     3721 ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg
     3781 cggcactgaa caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat
     3841 tgaccacgta ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct
     3901 cggccaccaa gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg
     3961 aaagtgtctt ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat
     4021 gcagcagcat tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt
     4081 tgcgttccgt ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg
     4141 actgcgtggc catgcttatc tccatgcggt agggtgccgc acggttgcgg caccatgcgc
     4201 aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc
     4261 aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg
     4321 ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc
     4381 tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg
     4441 ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg
     4501 ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg
     4561 gggcaggcgg gcgggcttcg ccttcgactg cccccactcg cataggcttg ggtcgttcca
     4621 ggcgcgtcaa ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt
     4681 ggtgtccaac cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa
     4741 attaaaaaaa ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg
     4801 tcgaaggctg ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct
     4861 gtccatcagc ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc
     4921 cgctcgcggc catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg
     4981 gcgaagcccg gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg
     5041 cgaagcaaag tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg
     5101 cgctgccccc gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc
     5161 gatcatgcga tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc
     5221 ctaccgcgag cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg
     5281 ggcggccagc agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg
     5341 gcgcaaggac gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa
     5401 gtcggccagc caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga
     5461 caagtacggg gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca
     5521 catgaccgcg ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat
     5581 cggcaacaaa gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct
     5641 agggctgcaa cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta
     5701 cgaggccctg gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc
     5761 acgcgcctat gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac
     5821 gccggaagcc gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct
     5881 acaggccgcc gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc
     5941 ccgag
//
