LOCUS       40924_5764        4100 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             331..348
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             352..384
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             388..411
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     terminator      671..757
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      849..876
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        895..986
                     /label=AmpR promoter
     CDS             987..1844
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2018..2606
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2792..2932)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3199..4074)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
      361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatcgatgg
      421 atccgacctc gagatctgca gatggtacca tatgggaatt cgaagcttgg ctgttttggc
      481 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
      541 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
      601 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
      661 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      721 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
      781 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
      841 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt
      901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      961 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1021 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1081 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1141 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1201 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     1261 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1321 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1381 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1441 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1501 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1561 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1621 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1681 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1741 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1801 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2161 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2761 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2821 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     2881 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     2941 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3001 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3061 acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg
     3121 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt
     3181 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
     3241 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
     3301 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
     3361 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
     3421 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
     3481 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
     3541 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
     3601 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
     3661 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
     3721 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
     3781 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
     3841 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
     3901 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     3961 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     4021 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     4081 catactcccg ccattcagag
//
