LOCUS       62056_3475        6357 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(123..998)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1025..1309
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1353..1766
                     /codon_start=1
                     /label=Gam
                     /note="inhibitor of the host RecBCD nuclease in the lambda 
                     Red system"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1775..2557
                     /codon_start=1
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2557..3234
                     /codon_start=1
                     /label=Exo
                     /note="5' to 3' double-stranded DNA exonuclease in the 
                     lambda
                     Red system"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      3238..3482
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     CDS             complement(3608..4555)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(4603..4825)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(5532..6320)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tagcacgatc aacggcactg ttgcaaatag tcggtggtga taaacttatc atcccctttt
       61 gcttatggag ctgcacatga actcacattt ccccgaaaag tgccacctgc atcgatttat
      121 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc
      181 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca
      241 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg
      301 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
      361 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
      421 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
      481 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
      541 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
      601 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat
      661 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
      721 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
      781 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
      841 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
      901 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
      961 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
     1021 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
     1081 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
     1141 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
     1201 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
     1261 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
     1321 tgggaattcg agctctaagg aggttataaa aaatggatat taatactgaa actgagatca
     1381 agcaaaagca ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc
     1441 gatattttca cagctatttc aggagttcag ccatgaacgc ttattacatt caggatcgtc
     1501 ttgaggctca gagctgggcg cgtcactacc agcagctcgc ccgtgaagag aaagaggcag
     1561 aactggcaga cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg
     1621 atcatttgca acgccacggg gccagcaaaa aatccattac ccgtgcgttt gatgacgatg
     1681 ttgagtttca ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc
     1741 accaggttga tattgattca gaggtataaa acgaatgagt actgcactcg caacgctggc
     1801 tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac
     1861 tcttcgccag acggcattta aaggtgatgc cagcgatgcg cagttcatcg cattactgat
     1921 cgttgccaac cagtacggcc ttaatccgtg gacgaaagaa atttacgcct ttcctgataa
     1981 gcagaatggc atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa
     2041 ccagcagttt gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta
     2101 ccgcaaggac cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga
     2161 accattcaaa actcgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg
     2221 gatgttacgt cataaagcca tgattcagtg tgcccgtctg gccttcggat ttgctggtat
     2281 ctatgacaag gatgaagccg agcgcattgt cgaaaatact gcatacactg cagaacgtca
     2341 gccggaacgc gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct
     2401 gatcgccctg gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg
     2461 ccgcgacatt cgtgcatcgt cagaactgac acaggccgaa gcagtaaaag ctcttggatt
     2521 cctgaaacag aaagccgcag agcagaaggt ggcagcatga caccggacat tatcctgcag
     2581 cgtaccggga tcgatgtgag agctgtcgaa cagggggatg atgcgtggca caaattacgg
     2641 ctcggcgtca tcaccgcttc agaagttcac aacgtgatag caaaaccccg ctccggaaag
     2701 aagtggcctg acatgaaaat gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt
     2761 gtggctccgg aagttaacgc taaagcactg gcctggggaa aacagtacga gaacgacgcc
     2821 agaaccctgt ttgaattcac ttccggcgtg aatgttactg aatccccgat catctatcgc
     2881 gacgaaagta tgcgtaccgc ctgctctccc gatggtttat gcagtgacgg caacggcctt
     2941 gaactgaaat gcccgtttac ctcccgggat ttcatgaagt tccggctcgg tggtttcgag
     3001 gccataaagt cagcttacat ggcccaggtg cagtacagca tgtgggtgac gcgaaaaaat
     3061 gcctggtact ttgccaacta tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg
     3121 attgagcggg atgaaaagta catggcgagt tttgacgaga tcgtgccgga gttcatcgaa
     3181 aaaatggacg aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgatgacgc
     3241 atcctcacga taatatccgg gtaggcgcaa tcactttcgt ctactccgtt acaaagcgag
     3301 gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag cggctggctg
     3361 aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg agttaagaac
     3421 gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc caataccagt
     3481 agaaacagac gaagaatcca tgggtatgga cagttttccc tttgatatgt aacggtgaac
     3541 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
     3601 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
     3661 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
     3721 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
     3781 cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
     3841 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
     3901 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
     3961 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
     4021 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
     4081 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
     4141 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
     4201 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
     4261 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
     4321 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
     4381 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
     4441 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
     4501 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
     4561 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
     4621 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
     4681 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
     4741 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
     4801 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
     4861 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
     4921 gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc
     4981 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     5041 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     5101 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     5161 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
     5221 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
     5281 aggcagcggt atcatcaacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5401 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcttc acctagatcc
     5461 tttacgcgtt cgcggaactt cggaatagga acttcatgag ctcagccaat cgactggcga
     5521 gcggcatctt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     5581 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     5641 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     5701 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     5761 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     5821 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     5881 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     5941 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     6001 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     6061 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     6121 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     6181 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     6241 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     6301 gcgatcaccg cttccctcat gacattgcac tccaccgctg atgacatcag tcgatca
//
