LOCUS       40924_5689       10508 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBACr-AraT7940_rbs9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
  PUBMED    17700541
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control 
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
  PUBMED    19801975
REFERENCE   3  (bases 1 to 10508)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates 
            and chemical 'wires'
  JOURNAL   Nature 469 (7329), 212-215 (2011)
  PUBMED    21150903
REFERENCE   4  (bases 1 to 10508)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 10508)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 10508)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Syst. 
            Biol. 3, 133 (2007)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2009"; volume: "27"; issue: "10"; pages: 
            "946-950"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nature"; 
            date: "2011"; volume: "469"; issue: "7329"; pages: "212-215"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (06-JAN-2012) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(105..980)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1007..1291
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1302..1334
                     /label=RBS9
                     /note="RBS9"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1335..4001
                     /gene="T7ptag"
                     /label=similar to T7ptag amberstop
                     /note="similar to T7ptag amberstop"
     gene            1335..4001
                     /gene="T7ptag"
                     /label=T7ptag
     misc_feature    1350..1352
                     /gene="T7ptag"
                     /label=amberstop
                     /note="amberstop"
     misc_feature    1668..1670
                     /gene="T7ptag"
                     /label=amberstop
                     /note="amberstop"
     CDS             4021..4050
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             4066..4083
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      4309..4355
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(4411..4799)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(4852..5643)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6049..6268
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6359..7111
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    7117..7367
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             7693..8865
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             8868..9836
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     gene            9912..10429
                     /gene="parC"
                     /label=parC
     misc_feature    9912..10385
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 ggatcgcggc cgccataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta
       61 tgctactccc tcgagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct
      121 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
      181 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
      241 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
      301 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
      361 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
      421 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
      481 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
      541 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
      601 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc
      661 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
      721 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
      781 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
      841 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
      901 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga
      961 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt
     1021 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa
     1081 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
     1141 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
     1201 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
     1261 tttttatcgc aactctctac tgtttctcca tacccggatc cctggacaac accgacgaac
     1321 tggagcctcc cggcatgatt accgtgcact agaataccat taacattgct aagaacgact
     1381 tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc
     1441 gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct
     1501 tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca
     1561 agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg
     1621 aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgtag gaaatcaagc
     1681 cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca
     1741 atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct
     1801 tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca
     1861 acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc
     1921 tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc
     1981 atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc
     2041 gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg
     2101 ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt
     2161 gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc
     2221 gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg
     2281 tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca
     2341 acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg
     2401 acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc
     2461 ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc
     2521 gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc
     2581 ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa
     2641 tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac
     2701 caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg
     2761 ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg
     2821 cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct
     2881 tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct
     2941 cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc
     3001 gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct
     3061 acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg
     3121 ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc
     3181 tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta
     3241 agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc
     3301 tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga
     3361 atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag
     3421 ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca
     3481 aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg
     3541 atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt
     3601 tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg
     3661 cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc
     3721 ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc
     3781 acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa
     3841 ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg
     3901 accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga
     3961 acctccgtga catcttagag tcggacttcg cgttcgcata agaattcgaa gcttgggccc
     4021 gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca tcatcatcat
     4081 cattgagttt aaacggtctc cagcttggct gttttggcgg atgagagaag attttcagcc
     4141 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     4201 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     4261 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     4321 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaactaattc
     4381 tgattcgcac gggcccatgg ctaattccca tgtcagccgt taagtgttcc tgtgtcactc
     4441 aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg cttgttgtcc
     4501 acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt cttataaaac
     4561 ttaaaacctt agaggctatt taagttgctg atttatatta attttattgt tcaaacatga
     4621 gagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt agtacgttag
     4681 ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga gagcttagta
     4741 cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat cttcagatcc
     4801 tctacgccgg acgcatcgtg gccggatctt gcgcaagcgg tctccaattc agaagaactc
     4861 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     4921 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tatccaacgc
     4981 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgttgaatc cagaaaagcg
     5041 gccattttcc accatgatat tcggcaagca ggcatcgcca tggatcacga cgagatcctc
     5101 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     5161 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     5221 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     5281 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggcgag atgacaggag
     5341 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     5401 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     5461 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     5521 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     5581 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     5641 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     5701 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     5761 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     5821 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     5881 gatagcccag tagctgacat tcatcaagct tatgatgtgg tctgtccttt tacagccagt
     5941 agtgctcgcc gcagtcgagc gacagggcga agccctcgag tgagcgagga agcaccaggg
     6001 aacagcactt atatattctg cttacacacg atgcctgaaa aaacttccct tggggttatc
     6061 cacttatcca cggggatatt tttataatta ttttttttat agtttttaga tcttcttttt
     6121 tagagcgcct tgtaggcctt tatccatgct ggttctagag aaggtgttgt gacaaattgc
     6181 cctttcagtg tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa ttgcccttaa
     6241 ccctgtgaca aattgccctc agaagaagct gttttttcac aaagttatcc ctgcttattg
     6301 actctttttt atttagtgtg acaatctaaa aacttgtcac acttcacatg gatctgtcat
     6361 ggcggaaaca gcggttatca atcacaagaa acgtaaaaat agcccgcgaa tcgtccagtc
     6421 aaacgacctc actgaggcgg catatagtct ctcccgggat caaaaacgta tgctgtatct
     6481 gttcgttgac cagatcagaa aatctgatgg caccctacag gaacatgacg gtatctgcga
     6541 gatccatgtt gctaaatatg ctgaaatatt cggattgacc tctgcggaag ccagtaagga
     6601 tatacggcag gcattgaaga gtttcgcggg gaaggaagtg gttttttatc gccctgaaga
     6661 ggatgccggc gatgaaaaag gctatgaatc ttttccttgg tttatcaaac gtgcgcacag
     6721 tccatccaga gggctttaca gtgtacatat caacccatat ctcattccct tctttatcgg
     6781 gttacagaac cggtttacgc agtttcggct tagtgaaaca aaagaaatca ccaatccgta
     6841 tgccatgcgt ttatacgaat ccctgtgtca gtatcgtaag ccggatggct caggcatcgt
     6901 ctctctgaaa atcgactgga tcatagagcg ttaccagctg cctcaaagtt accagcgtat
     6961 gcctgacttc cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca gcagaactcc
     7021 aatgcgcctc tcatacattg agaaaaagaa aggccgccag acgactcata tcgtattttc
     7081 cttccgcgat atcacttcca tgacgacagg atagtctgag ggttatctgt cacagatttg
     7141 agggtggttc gtcacatttg ttctgaccta ctgagggtaa tttgtcacag ttttgctgtt
     7201 tccttcagcc tgcatggatt ttctcatact ttttgaactg taatttttaa ggaagccaaa
     7261 tttgagggca gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg tgacctgata
     7321 tcgggggtta gttcgtcatc attgatgagg gttgattatc acagtttatt actctgaatt
     7381 ggctatccgc gtgtgtacct ctacctggag tttttcccac ggtggatatt tcttcttgcg
     7441 ctgagcgtaa gagctatctg acagaacagt tcttctttgc ttcctcgcca gttcgctcgc
     7501 tatgctcggt tacacggctg cggcgagcgc tagtgataat aagtgactga ggtatgtgct
     7561 cttcttatct ccttttgtag tgttgctctt attttaaaca actttgcggt tttttgatga
     7621 ctttgcgatt ttgttgttgc tttgcagtaa attgcaagat ttaataaaaa aacgcaaagc
     7681 aatgattaaa ggatgttcag aatgaaactc atggaaacac ttaaccagtg cataaacgct
     7741 ggtcatgaaa tgacgaaggc tatcgccatt gcacagttta atgatgacag cccggaagcg
     7801 aggaaaataa cccggcgctg gagaataggt gaagcagcgg atttagttgg ggtttcttct
     7861 caggctatca gagatgccga gaaagcaggg cgactaccgc acccggatat ggaaattcga
     7921 ggacgggttg agcaacgtgt tggttataca attgaacaaa ttaatcatat gcgtgatgtg
     7981 tttggtacgc gattgcgacg tgctgaagac gtatttccac cggtgatcgg ggttgctgcc
     8041 cataaaggtg gcgtttacaa aacctcagtt tctgttcatc ttgctcagga tctggctctg
     8101 aaggggctac gtgttttgct cgtggaaggt aacgaccccc agggaacagc ctcaatgtat
     8161 cacggatggg taccagatct tcatattcat gcagaagaca ctctcctgcc tttctatctt
     8221 ggggaaaagg acgatgtcac ttatgcaata aagcccactt gctggccggg gcttgacatt
     8281 attccttcct gtctggctct gcaccgtatt gaaactgagt taatgggcaa atttgatgaa
     8341 ggtaaactgc ccaccgatcc acacctgatg ctccgactgg ccattgaaac tgttgctcat
     8401 gactatgatg tcatagttat tgacagcgcg cctaacctgg gtatcggcac gattaatgtc
     8461 gtatgtgctg ctgatgtgct gattgttccc acgcctgctg agttgtttga ctacacctcc
     8521 gcactgcagt ttttcgatat gcttcgtgat ctgctcaaga acgttgatct taaagggttc
     8581 gagcctgatg tacgtatttt gcttaccaaa tacagcaata gtaatggctc tcagtccccg
     8641 tggatggagg agcaaattcg ggatgcctgg ggaagcatgg ttctaaaaaa tgttgtacgt
     8701 gaaacggatg aagttggtaa aggtcagatc cggatgagaa ctgtttttga acaggccatt
     8761 gatcaacgct cttcaactgg tgcctggaga aatgctcttt ctatttggga acctgtctgc
     8821 aatgaaattt tcgatcgtct gattaaacca cgctgggaga ttagataatg aagcgtgcgc
     8881 ctgttattcc aaaacatacg ctcaatactc aaccggttga agatacttcg ttatcgacac
     8941 cagctgcccc gatggtggat tcgttaattg cgcgcgtagg agtaatggct cgcggtaatg
     9001 ccattacttt gcctgtatgt ggtcgggatg tgaagtttac tcttgaagtg ctccggggtg
     9061 atagtgttga gaagacctct cgggtatggt caggtaatga acgtgaccag gagctgctta
     9121 ctgaggacgc actggatgat ctcatccctt cttttctact gactggtcaa cagacaccgg
     9181 cgttcggtcg aagagtatct ggtgtcatag aaattgccga tgggagtcgc cgtcgtaaag
     9241 ctgctgcact taccgaaagt gattatcgtg ttctggttgg cgagctggat gatgagcaga
     9301 tggctgcatt atccagattg ggtaacgatt atcgcccaac aagtgcttat gaacgtggtc
     9361 agcgttatgc aagccgattg cagaatgaat ttgctggaaa tatttctgcg ctggctgatg
     9421 cggaaaatat ttcacgtaag attattaccc gctgtatcaa caccgccaaa ttgcctaaat
     9481 cagttgttgc tcttttttct caccccggtg aactatctgc ccggtcaggt gatgcacttc
     9541 aaaaagcctt tacagataaa gaggaattac ttaagcagca ggcatctaac cttcatgagc
     9601 agaaaaaagc tggggtgata tttgaagctg aagaagttat cactctttta acttctgtgc
     9661 ttaaaacgtc atctgcatca agaactagct taagctcacg acatcagttt gctcctggag
     9721 cgacagtatt gtataagggc gataaaatgg tgcttaacct ggacaggtct cgtgttccaa
     9781 ctgagtgtat agagaaaatt gaggccattc ttaaggaact tgaaaagcca gcaccctgat
     9841 gcgaccacgt tttagtctac gtttatctgt ctttacttaa tgtcctttgt tacaggccag
     9901 aaagcataac tggcctgaat attctctctg ggcccactgt tccacttgta tcgtcggtct
     9961 gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
    10021 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
    10081 cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta
    10141 gtctgggacc atggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc
    10201 cactcgtatc gtcggtctga ttattagtct ggaaccacgg tcccactcgt atcgtcggtc
    10261 tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga
    10321 ccacgatccc actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccacttgta
    10381 ttgtcgatca gactatcagc gtgagactac gattccatca atgcctgtca agggcaagta
    10441 ttgacatgtc gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct
    10501 gtggattg
//
