LOCUS       pBACgus4x-1.        8066 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid encoding beta-glucuronidase, with two 
            polyhedrin and two p10 promoters, for the simultaneous expression of
            up to four proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pBACgus4x-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1170..2978)
                     /label=GUS
                     /note="beta-glucuronidase"
     promoter        complement(2987..3302)
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     polyA_signal    complement(3320..3368)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3371..3397
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(3400..3509)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    3642..3652
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(3660..3751)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3776..3885
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    3893..3904
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    3905..3953
                     /label=poly(A) signal
                     /note="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        3991..4082
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4086..4115
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             4116..4133
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     4170..5527
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5790..6378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6552..7409)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7410..7514)
                     /label=AmpR promoter
     rep_origin      7541..7996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatcgtaacc acacaaaaaa ccaacacaca gttctaatga aaataaagaa
     3361 cttttattat cctcaggtct agctggaatt cagatctgtg attgtaaata aaatgtaatt
     3421 tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta tttaactata
     3481 atatattgtg ttgggttgaa ttaaaggtcc cggcatcctc aaatgcataa tttcatagtc
     3541 ccccttgttg taagtgatgc gtatttctga atctttgtaa aatagcacac aagactccaa
     3601 cgcgtttggc gttttatttt cttgctcgac tctagtttat taggcctcta gagatccgta
     3661 tttataggtt tttttattac aaaactgtta cgaaaacagt aaaatactta tttatttgcg
     3721 agatggttat cattttaatt atctccatga tccaataacc tagaataaag ggcccgacct
     3781 ttaattcaac ccaacacaat atattatagt taaataagaa ttattatcaa atcatttgta
     3841 tattaattaa aatactatac tgtaaattac attttattta caatcacaga tccccgggac
     3901 tagtaataaa agatgtttat tttcattaga tgtgtgtgtt ggttttttgt gtctagtaat
     3961 aaaacctaga ggatcgatcc cccggatctg atcatggaga taattaaaat gataaccatc
     4021 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
     4081 atatcggatc ctctaagctt gcggccgcac tcgagcacca ccaccaccac cactaaccta
     4141 ggtagctgag cgcatgcaag ctgatccggg ttattagtac atttattaag cgctagattc
     4201 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
     4261 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
     4321 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
     4381 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
     4441 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
     4501 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
     4561 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg
     4621 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
     4681 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
     4741 cgcgatcaaa tttgtagttg agctttttgg aattatttct gattgcgggc gtttttgggc
     4801 gggtttcaat ctaactgtgc ccgattttaa ttcagacaac acgttagaaa gcgatggtgc
     4861 aggcggtggt aacatttcag acggcaaatc tactaatggc ggcggtggtg gagctgatga
     4921 taaatctacc atcggtggag gcgcaggcgg ggctggcggc ggaggcggag gcggaggtgg
     4981 tggcggtgat gcagacggcg gtttaggctc aaatgtctct ttaggcaaca cagtcggcac
     5041 ctcaactatt gtactggttt cgggcgccgt ttttggtttg accggtctga gacgagtgcg
     5101 atttttttcg tttctaatag cttccaacaa ttgttgtctg tcgtctaaag gtgcagcggg
     5161 ttgaggttcc gtcggcattg gtggagcggg cggcaattca gacatcgatg gtggtggtgg
     5221 tggtggaggc gctggaatgt taggcacggg agaaggtggt ggcggcggtg ccgccggtat
     5281 aatttgttct ggtttagttt gttcgcgcac gattgtgggc accggcgcag gcgccgctgg
     5341 ctgcacaacg gaaggtcgtc tgcttcgagg cagcgcttgg ggtggtggca attcaatatt
     5401 ataattggaa tacaaatcgt aaaaatctgc tataagcatt gtaatttcgc tatcgtttac
     5461 cgtgccgata tttaacaacc gctcaatgta agcaattgta ttgtaaagag attgtctcaa
     5521 gctcggatcg atcccgcacg ccgataacaa gccttttcat ttttactaca gcattgtagt
     5581 ggcgagacac ttcgctgtcg tcgaggttta aacgcttcct cgctcactga ctcgctgcgc
     5641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     6241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     7561 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7621 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7681 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7741 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7801 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7861 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7921 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7981 ttaacgttta caatttccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     8041 gtgcgggcct cttcgctatt acgcca
//
