LOCUS       pBACgus-6.        7729 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the gp64 promoter, encoding 
            beta-glucuronidase plus an N-terminal signal 
            sequence-6xHis-thrombin-S-Tag-enterokinase cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBACgus-6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1170..2978)
                     /label=GUS
                     /note="beta-glucuronidase"
     promoter        complement(2987..3302)
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3315..3520
                     /label=gp64 promoter
                     /note="promoter for the baculovirus gp64 glycoprotein gene"
     sig_peptide     3521..3580
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     CDS             3590..3607
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             3617..3634
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             3638..3682
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             3698..3712
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3714..3778
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3779..3796
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     3833..5190
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5453..6041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6215..7072)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7073..7177)
                     /label=AmpR promoter
     rep_origin      7204..7659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgtcga ctgagcgtcc gtgttcatga tcccgttttt ataacagcca
     3361 gataaaaata atcttatcaa ttaagataaa aagataagat tattaatcta acaacgtgcc
     3421 ttgtgtcacg taggccagat aacggtcggg tatataagac gcctcaacgc tactagtaaa
     3481 tcagtcacac caaggcttca ataaggaaca cacaagcaag atggtaagcg ctattgtttt
     3541 atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg gccatggtcc atcatcacca
     3601 ccatcactcc gcgggtttgg tgcccagagg cagcggcaaa gaaacggcgg cggcgaaatt
     3661 tgaacgccaa cacatggaca gcgctagcgg tggaggtgat gacgacgaca agagcccggg
     3721 cttctcctca aaaggcctgg atccgaattc gagctcgaag cttgcggccg cactcgagca
     3781 ccaccaccac caccactaac ctaggtagct gagcgcatgc aagctgatcc gggttattag
     3841 tacatttatt aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat
     3901 tttaataatt cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga
     3961 ttttcagcgt ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg
     4021 tttcgattag tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga
     4081 ttttcgctca acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata
     4141 ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt
     4201 gtatttcttt catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataga
     4261 gcttggacat atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc
     4321 caaccctcgt cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta
     4381 ttaattgtgt cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt
     4441 tctgattgcg ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac
     4501 aacacgttag aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat
     4561 ggcggcggtg gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc
     4621 ggcggaggcg gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc
     4681 tctttaggca acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt
     4741 ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt
     4801 ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat
     4861 tcagacatcg atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt
     4921 ggtggcggcg gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg
     4981 ggcaccggcg caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct
     5041 tggggtggtg gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc
     5101 attgtaattt cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt
     5161 gtattgtaaa gagattgtct caagctcgga tcgatcccgc acgccgataa caagcctttt
     5221 catttttact acagcattgt agtggcgaga cacttcgctg tcgtcgaggt ttaaacgctt
     5281 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5341 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5401 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5461 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5521 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5581 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5641 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5701 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5761 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5821 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5881 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5941 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6001 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6061 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6121 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6181 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6241 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6301 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6361 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6421 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6481 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6541 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6601 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6661 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6721 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6781 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6841 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6901 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6961 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7021 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7081 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7141 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7201 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7261 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7321 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7381 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7621 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca ttcaggctgc
     7681 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgcca
//
