LOCUS       V009306                 9077 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009306
VERSION     V009306
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Schoenborn MA, Lippmann W, Amemiya CT.
  TITLE     BAC Cloning Plasmid: pBAC-VM4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Schoenborn MA, Lippmann W, Amemiya CT.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2003) Molecular Genetics, Benaroya Research
            Institute at Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101, USA
REFERENCE   3  (bases 1 to 9077)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-MAY-2003) Molecular Genetics, Benaroya Research Institute at
            Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(248..1663)
                     /gene="sacB"
                     /label="Levansucrase"
                     /note="Levansucrase from Bacillus amyloliquefaciens.
                     Accession#: P21130"
     promoter        1763..1781
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    1792..1848
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1891..1907)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1915..1931)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1939..1969)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1984..2005)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2228..2884)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2885..2987)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3845..4459
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4535..4754
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             4845..5597
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     misc_feature    5603..5853
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6179..7351
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             7354..8322
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    8398..8871
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg
       61 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca
      121 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatctaccca ctagtcaatt
      181 caccatcttg ttgagaaata aaagaaaatg ccaatgaagt atcggcattt tctttttgct
      241 gttattagtt gactgtcagc tgtccttgct ccaggatgct gtttttgaca acggatgttt
      301 tattgccttt gatgttcatt aagaagcttg gcgcaaatgt tgccttttta tcctcgaaga
      361 agcctctgtt tgtcatgtag cttgtgataa ccacattgtt gcctttggct tgcggcactg
      421 cgaagtgaga gtaagtgaat gtcacatcgt ttggatcaag acccatttgc agcacaagcc
      481 ctgttttgtt cagcggcttg taagggccgg ttaaagagtt tgatacataa ccaagcatgt
      541 aaatatcgtt tgagttaata ccatcgatcg tcatttttga accgcgtgaa tcagtgaaca
      601 agtaccattt gccgttcatt ttgaaaacat tcgcgcgctc gatttcatca gttaccgtgt
      661 ttgaagtgat cagcggcttc attacttttt tcaatgtgta atcattattt aactctatga
      721 taccgagggc gccgttcgct aactcagcat cgcgtttttt agcgctctgc tgaagcttct
      781 ggctttcttt acggaagaag ttcgtgccgc cgccgtagta cgctttgtta aataaagatt
      841 cttcgccttg gtatccgttt tctgttcccg tgttggcttc gaatacaagg tatttatggc
      901 ctttgtcttc aacgtagtga gggtctctca gcgtatggtt gtcgccggat gtataattgc
      961 cttcatcgat aaactgctga acgttctgat atgtttttcc gtctccgtca aaaatcgttt
     1021 tgtgatcttc cactccgttg attttgagtg tgtcatcaga ttttgacaca tttacctgcg
     1081 ctgttgtcag gctttgtttg ccgtaatgtt taccggaata gtcagtgtag aataaacgga
     1141 tttttccgtc agatgtaaag gttgcagaac cggaccattc ttgcgtctga tctttcagga
     1201 tcggatcgtt ggcgtcgaac ttatcgctgt ctttaaagac acggcccgcg tttttccagc
     1261 tgtcgattga gttgtcgccg accttttgat aaaacatgta gattgatgtg tcatcagcgt
     1321 ctttcgggct tcccgcaaga gcaaacacaa cgtgatagcc gttgtattca gctactgttc
     1381 cgtcagcgtt ttgcagcggc cagctgtccc acacatcaag tccttttgca gactcaatat
     1441 ttttaatcgt tgattgatcg aattgaggca cttggtattt ttcgttttgc tgctgtttag
     1501 ggatctgcag catatcatgg cgtgtaatat gagagacgcc gtacgtttct ttgtatgctt
     1561 tttggttatt ttctttcgcg aaggcttgag tcgctcctcc tgccagaagt gcagtcgtaa
     1621 aagtcagaac tgtggcttgt tttacaattt ttttgatgtt catgttcatg tctccttctg
     1681 tatgtactgt tttttgcgat ctgccgtttc gatcctcccg aattgactag tgggtaggcc
     1741 tggcggccgc ctggccgtcg acatttaggt gacactatag aaggatccgc ggaattcgag
     1801 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttga gtattctata
     1861 gtctcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gaaccccttg cggccgcccg
     2101 ggccgtcgac caattctcat gtttgacagc ttatcatcga atttctgcca ttcatccgct
     2161 tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa
     2221 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     2281 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2341 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2401 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     2461 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2521 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2581 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2641 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     2701 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2761 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2821 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2881 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2941 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     3001 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
     3061 cgaagtgatc ttccgtcaca ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt
     3121 cgcacaggaa ggacagagaa agcgcggatc tgggaagtga cggacagaac ggtcaggacc
     3181 tggattgggg aggcggttgc cgccgctgct gctgacggtg tgacgttctc tgttccggtc
     3241 acaccacata cgttccgcca ttcctatgcg atgcacatgc tgtatgccgg tataccgctg
     3301 aaagttctgc aaagcctgat gggacataag tccatcagtt caacggaagt ctacacgaag
     3361 gtttttgcgc tggatgtggc tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat
     3421 gcggttgcga tgctgaaaca attatcctga gaataaatgc cttggccttt atatggaaat
     3481 gtggaactga gtggatatgc tgtttttgtc tgttaaacag agaagctggc tgttatccac
     3541 tgagaagcga acgaaacagt cgggaaaatc tcccattatc gtagagatcc gcattattaa
     3601 tctcaggagc ctgtgtagcg tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg
     3661 taacgaaaac gatttgaata tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt
     3721 tgaagtggag cggattatgt cagcaatgga cagaacaacc taatgaacac agaaccatga
     3781 tgtggtctgt ccttttacag ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc
     3841 tcggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
     3901 aacgccgtcg aagccgtgtg cgagacaccg cggccggccg ccggcgttgt ggatacctcg
     3961 cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact
     4021 cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct
     4081 ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt
     4141 ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca
     4201 gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc
     4261 tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc
     4321 cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc
     4381 ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc
     4441 ccccttctcg aaccctcccg gtcgagtgag cgaggaagca ccagggaaca gcacttatat
     4501 attctgctta cacacgatgc ctgaaaaaac ttcccttggg gttatccact tatccacggg
     4561 gatattttta taattatttt ttttatagtt tttagatctt cttttttaga gcgccttgta
     4621 ggcctttatc catgctggtt ctagagaagg tgttgtgaca aattgccctt tcagtgtgac
     4681 aaatcaccct caaatgacag tcctgtctgt gacaaattgc ccttaaccct gtgacaaatt
     4741 gccctcagaa gaagctgttt tttcacaaag ttatccctgc ttattgactc ttttttattt
     4801 agtgtgacaa tctaaaaact tgtcacactt cacatggatc tgtcatggcg gaaacagcgg
     4861 ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt ccagtcaaac gacctcactg
     4921 aggcggcata tagtctctcc cgggatcaaa aacgtatgct gtatctgttc gttgaccaga
     4981 tcagaaaatc tgatggcacc ctacaggaac atgacggtat ctgcgagatc catgttgcta
     5041 aatatgctga aatattcgga ttgacctctg cggaagccag taaggatata cggcaggcat
     5101 tgaagagttt cgcggggaag gaagtggttt tttatcgccc tgaagaggat gccggcgatg
     5161 aaaaaggcta tgaatctttt ccttggttta tcaaacgtgc gcacagtcca tccagagggc
     5221 tttacagtgt acatatcaac ccatatctca ttcccttctt tatcgggtta cagaaccggt
     5281 ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa tccgtatgcc atgcgtttat
     5341 acgaatccct gtgtcagtat cgtaagccgg atggctcagg catcgtctct ctgaaaatcg
     5401 actggatcat agagcgttac cagctgcctc aaagttacca gcgtatgcct gacttccgcc
     5461 gccgcttcct gcaggtctgt gttaatgaga tcaacagcag aactccaatg cgcctctcat
     5521 acattgagaa aaagaaaggc cgccagacga ctcatatcgt attttccttc cgcgatatca
     5581 cttccatgac gacaggatag tctgagggtt atctgtcaca gatttgaggg tggttcgtca
     5641 catttgttct gacctactga gggtaatttg tcacagtttt gctgtttcct tcagcctgca
     5701 tggattttct catacttttt gaactgtaat ttttaaggaa gccaaatttg agggcagttt
     5761 gtcacagttg atttccttct ctttcccttc gtcatgtgac ctgatatcgg gggttagttc
     5821 gtcatcattg atgagggttg attatcacag tttattactc tgaattggct atccgcgtgt
     5881 gtacctctac ctggagtttt tcccacggtg gatatttctt cttgcgctga gcgtaagagc
     5941 tatctgacag aacagttctt ctttgcttcc tcgccagttc gctcgctatg ctcggttaca
     6001 cggctgcggc gagcgctagt gataataagt gactgaggta tgtgctcttc ttatctcctt
     6061 ttgtagtgtt gctcttattt taaacaactt tgcggttttt tgatgacttt gcgattttgt
     6121 tgttgctttg cagtaaattg caagatttaa taaaaaaacg caaagcaatg attaaaggat
     6181 gttcagaatg aaactcatgg aaacacttaa ccagtgcata aacgctggtc atgaaatgac
     6241 gaaggctatc gccattgcac agtttaatga tgacagcccg gaagcgagga aaataacccg
     6301 gcgctggaga ataggtgaag cagcggattt agttggggtt tcttctcagg ctatcagaga
     6361 tgccgagaaa gcagggcgac taccgcaccc ggatatggaa attcgaggac gggttgagca
     6421 acgtgttggt tatacaattg aacaaattaa tcatatgcgt gatgtgtttg gtacgcgatt
     6481 gcgacgtgct gaagacgtat ttccaccggt gatcggggtt gctgcccata aaggtggcgt
     6541 ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg gctctgaagg ggctacgtgt
     6601 tttgctcgtg gaaggtaacg acccccaggg aacagcctca atgtatcacg gatgggtacc
     6661 agatcttcat attcatgcag aagacactct cctgcctttc tatcttgggg aaaaggacga
     6721 tgtcacttat gcaataaagc ccacttgctg gccggggctt gacattattc cttcctgtct
     6781 ggctctgcac cgtattgaaa ctgagttaat gggcaaattt gatgaaggta aactgcccac
     6841 cgatccacac ctgatgctcc gactggccat tgaaactgtt gctcatgact atgatgtcat
     6901 agttattgac agcgcgccta acctgggtat cggcacgatt aatgtcgtat gtgctgctga
     6961 tgtgctgatt gttcccacgc ctgctgagtt gtttgactac acctccgcac tgcagttttt
     7021 cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa gggttcgagc ctgatgtacg
     7081 tattttgctt accaaataca gcaatagtaa tggctctcag tccccgtgga tggaggagca
     7141 aattcgggat gcctggggaa gcatggttct aaaaaatgtt gtacgtgaaa cggatgaagt
     7201 tggtaaaggt cagatccgga tgagaactgt ttttgaacag gccattgatc aacgctcttc
     7261 aactggtgcc tggagaaatg ctctttctat ttgggaacct gtctgcaatg aaattttcga
     7321 tcgtctgatt aaaccacgct gggagattag ataatgaagc gtgcgcctgt tattccaaaa
     7381 catacgctca atactcaacc ggttgaagat acttcgttat cgacaccagc tgccccgatg
     7441 gtggattcgt taattgcgcg cgtaggagta atggctcgcg gtaatgccat tactttgcct
     7501 gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc ggggtgatag tgttgagaag
     7561 acctctcggg tatggtcagg taatgaacgt gaccaggagc tgcttactga ggacgcactg
     7621 gatgatctca tcccttcttt tctactgact ggtcaacaga caccggcgtt cggtcgaaga
     7681 gtatctggtg tcatagaaat tgccgatggg agtcgccgtc gtaaagctgc tgcacttacc
     7741 gaaagtgatt atcgtgttct ggttggcgag ctggatgatg agcagatggc tgcattatcc
     7801 agattgggta acgattatcg cccaacaagt gcttatgaac gtggtcagcg ttatgcaagc
     7861 cgattgcaga atgaatttgc tggaaatatt tctgcgctgg ctgatgcgga aaatatttca
     7921 cgtaagatta ttacccgctg tatcaacacc gccaaattgc ctaaatcagt tgttgctctt
     7981 ttttctcacc ccggtgaact atctgcccgg tcaggtgatg cacttcaaaa agcctttaca
     8041 gataaagagg aattacttaa gcagcaggca tctaaccttc atgagcagaa aaaagctggg
     8101 gtgatatttg aagctgaaga agttatcact cttttaactt ctgtgcttaa aacgtcatct
     8161 gcatcaagaa ctagtttaag ctcacgacat cagtttgctc ctggagcgac agtattgtat
     8221 aagggcgata aaatggtgct taacctggac aggtctcgtg ttccaactga gtgtatagag
     8281 aaaattgagg ccattcttaa ggaacttgaa aagccagcac cctgatgcga ccacgtttta
     8341 gtctacgttt atctgtcttt acttaatgtc ctttgttaca ggccagaaag cataactggc
     8401 ctgaatattc tctctgggcc cactgttcca cttgtatcgt cggtctgata atcagactgg
     8461 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     8521 tatcgtcggt ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgataa
     8581 tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccatgg
     8641 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg
     8701 gtctgattat tagtctggaa ccacggtccc actcgtatcg tcggtctgat tattagtctg
     8761 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac gatcccactc
     8821 gtgttgtcgg tctgattatc ggtctgggac cacggtccca cttgtattgt cgatcagact
     8881 atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg caagtattga catgtcgtcg
     8941 taacctgtag aacggagtaa cctcggtgtg cggttgtatg cctgctgtgg attgctgctg
     9001 tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg acaaaatacc tggttaccca
     9061 ggccgtgccg gcacgtt
//
