LOCUS       pBAC4x-1.        5917 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with two polyhedrin and two p10 
            promoters, for the simultaneous expression of up to four proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC4x-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(1171..1219)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    1222..1248
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1251..1360)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1493..1503
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1511..1602)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        1627..1736
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1744..1755
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    1756..1804
                     /label=poly(A) signal
                     /note="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        1842..1933
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    1937..1966
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1967..1984
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     2021..3378
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3641..4229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4403..5260)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5261..5365)
                     /label=AmpR promoter
     rep_origin      5392..5847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcgtaac cacacaaaaa accaacacac agttctaatg
     1201 aaaataaaga acttttatta tcctcaggtc tagctggaat tcagatctgt gattgtaaat
     1261 aaaatgtaat ttacagtata gtattttaat taatatacaa atgatttgat aataattctt
     1321 atttaactat aatatattgt gttgggttga attaaaggtc ccggcatcct caaatgcata
     1381 atttcatagt cccccttgtt gtaagtgatg cgtatttctg aatctttgta aaatagcaca
     1441 caagactcca acgcgtttgg cgttttattt tcttgctcga ctctagttta ttaggcctct
     1501 agagatccgt atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt
     1561 atttatttgc gagatggtta tcattttaat tatctccatg atccaataac ctagaataaa
     1621 gggcccgacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
     1681 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcacag
     1741 atccccggga ctagtaataa aagatgttta ttttcattag atgtgtgtgt tggttttttg
     1801 tgtctagtaa taaaacctag aggatcgatc ccccggatct gatcatggag ataattaaaa
     1861 tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa
     1921 aaaacctata aatatcggat cctctaagct tgcggccgca ctcgagcacc accaccacca
     1981 ccactaacct aggtagctga gcgcatgcaa gctgatccgg gttattagta catttattaa
     2041 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
     2101 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
     2161 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
     2221 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
     2281 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
     2341 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
     2401 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc ttggacatat
     2461 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
     2521 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
     2581 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg
     2641 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa
     2701 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt
     2761 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga
     2821 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac
     2881 acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg
     2941 agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa
     3001 ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat
     3061 ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt
     3121 gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca
     3181 ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc
     3241 aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg
     3301 ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga
     3361 gattgtctca agctcggatc gatcccgcac gccgataaca agccttttca tttttactac
     3421 agcattgtag tggcgagaca cttcgctgtc gtcgaggttt aaacgcttcc tcgctcactg
     3481 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3541 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3601 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3661 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3721 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3781 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3841 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3901 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3961 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4021 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4081 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4141 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4201 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4261 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4321 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4381 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4441 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4501 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4561 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4621 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4681 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4741 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4801 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4861 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4921 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4981 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5041 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5101 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5161 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5221 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5281 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5341 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     5401 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5461 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5521 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5581 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5641 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5701 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5761 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5821 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg
     5881 aagggcgatc ggtgcgggcc tcttcgctat tacgcca
//
