LOCUS       40924_5544       10494 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAC-SCenhanced, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Creating Single-Copy Genetic Circuits
  JOURNAL   Mol. Cell 63 (2), 329-336 (2016)
  PUBMED    27425413
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St,
            MIT Building E25-Rm302, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 10494)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell";
            date: "2016"; volume: "63"; issue: "2"; pages: "329-336"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St, MIT 
            Building E25-Rm302, Cambridge, MA 02142, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(105..1073)
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(1076..2248)
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(2574..2824)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(2830..3582)
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     rep_origin      complement(3673..3892)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      complement(3968..4582)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        5440..5542
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5543..6199
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      complement(6455..6549)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6612..7325)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(7326..7349)
                     /label=G5
                     /note="G5"
                     /regulatory_class="ribosome_binding_site"
     RBS             7331..7339
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      complement(7367..7440)
                     /label=PLtetO
                     /note="PLtetO"
                     /regulatory_class="promoter"
     promoter        complement(7367..7440)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     prim_transcript 7386
                     /label=transcription start
                     /note="transcription start"
     protein_bind    7397..7415
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7422..7440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(7486..8565)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(8602..8675)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     promoter        8707..8736
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     prim_transcript 8742
                     /label=transcription start
                     /note="transcription start"
     protein_bind    8744..8760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8807..9427
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      9473..9547
                     /label=Ptrc-2
                     /note="Ptrc-2"
                     /regulatory_class="promoter"
     promoter        9486..9515
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    9523..9539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     prim_transcript 9523
                     /label=transcription start
                     /note="transcription start"
     regulatory      9555..9581
                     /label=M2
                     /note="M2"
                     /regulatory_class="ribosome_binding_site"
     RBS             9568..9576
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9582..10289
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      10352..10438
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagtggggcc cagagagaat attcaggcca gttatgcttt ctggcctgta acaaaggaca
       61 ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc tggcttttca
      121 agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg ggacctgtcc
      181 aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc aaactgatgt
      241 cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt taaaagagtg
      301 ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag gttagatgcc
      361 tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc acctgaccgg
      421 gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa tttggcggtg
      481 ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag cgcagaaata
      541 tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc ataagcactt
      601 gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc atccagctcg
      661 ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg gcgactccca
      721 tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg ttgaccagtc
      781 agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc ctggtcacgt
      841 tcattacctg accatacccg agaggtcttc tcaacactat caccccggag cacttcaaga
      901 gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg agccattact
      961 cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa cgaagtatct
     1021 tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt cattatctaa
     1081 tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt tcccaaatag
     1141 aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt tcaaaaacag
     1201 ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca ttttttagaa
     1261 ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga gagccattac
     1321 tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta agatcaacgt
     1381 tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag tcaaacaact
     1441 cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc gtgccgatac
     1501 ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca gtttcaatgg
     1561 ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat ttgcccatta
     1621 actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc cccggccagc
     1681 aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa ggcaggagag
     1741 tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag gctgttccct
     1801 gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga tcctgagcaa
     1861 gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc ccgatcaccg
     1921 gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc atatgattaa
     1981 tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc atatccgggt
     2041 gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc ccaactaaat
     2101 ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg ctgtcatcat
     2161 taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg cactggttaa
     2221 gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt tttttattaa
     2281 atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa accgcaaagt
     2341 tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc tcagtcactt
     2401 attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac tggcgaggaa
     2461 gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga aatatccacc
     2521 gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt aataaactgt
     2581 gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca catgacgaag
     2641 ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc ttaaaaatta
     2701 cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa ctgtgacaaa
     2761 ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg acagataacc
     2821 ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga tatgagtcgt
     2881 ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc tgttgatctc
     2941 attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt aactttgagg
     3001 cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg agccatccgg
     3061 cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg tgatttcttt
     3121 tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga agggaatgag
     3181 atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac gtttgataaa
     3241 ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc gataaaaaac
     3301 cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg cttccgcaga
     3361 ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac cgtcatgttc
     3421 ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca tacgtttttg
     3481 atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga ttcgcgggct
     3541 atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc catgtgaagt
     3601 gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag ggataacttt
     3661 gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa tttgtcacag
     3721 acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc acaacacctt
     3781 ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga tctaaaaact
     3841 ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca agggaagttt
     3901 tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct tcctcgctca
     3961 ctcgaccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca
     4021 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa
     4081 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc
     4141 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc
     4201 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg
     4261 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc
     4321 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc
     4381 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca
     4441 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cggccgcggt
     4501 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
     4561 gcccagcggc gagggcaacc agccgagggc ttcgccctgt cgctcgactg cggcgagcac
     4621 tactggctgt aaaaggacag accacatcat ggttctgtgt tcattaggtt gttctgtcca
     4681 ttgctgacat aatccgctcc acttcaacgt aacaccgcac gaagatttct attgttcctg
     4741 aaggcatatt caaatcgttt tcgttaccgc ttgcaggcat catgacagaa cactacttcc
     4801 tataaacgct acacaggctc ctgagattaa taatgcggat ctctacgata atgggagatt
     4861 ttcccgactg tttcgttcgc ttctcagtgg ataacagcca gcttctctgt ttaacagaca
     4921 aaaacagcat atccactcag ttccacattt ccatataaag gccaaggcat ttattctcag
     4981 gataattgtt tcagcatcgc aaccgcatca gactccggca tcgcaaactg cacccggtgc
     5041 cgggcagcca catccagcgc aaaaaccttc gtgtagactt ccgttgaact gatggactta
     5101 tgtcccatca ggctttgcag aactttcagc ggtataccgg catacagcat gtgcatcgca
     5161 taggaatggc ggaacgtatg tggtgtgacc ggaacagaga acgtcacacc gtcagcagca
     5221 gcggcggcaa ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc
     5281 cgcgctttct ctgtccttcc tgtgcgacgg ttacgccgct ccatgagctt atcgcgaata
     5341 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
     5401 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
     5461 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     5521 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     5581 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     5641 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     5701 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     5761 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     5821 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     5881 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     5941 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     6001 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     6061 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     6121 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     6181 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     6241 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg
     6301 tcaaacatga gaattggtcg agacggtccc tagggcgata gatatcaacc ctggcgccgg
     6361 gggacaattg tgtagtcccg ggaacgagca tatggagcga cttaagcagt ccgagctcca
     6421 gacggctagc gtaatggtcg acgagtatgc ttggattctc accaataaaa aacgcccggc
     6481 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     6541 acaggagtcc aagcgacgtc tgtgcaagta ctactgttct gcagtcactt gaattcgata
     6601 cccagctgtt atttgtatag ttcatccatg ccatgtgtaa tcccagcagc ggttacaaac
     6661 tcaagaagga ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc agattgtgtg
     6721 gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caatcggagt attttgttga
     6781 taatggtctg ctagttgaac gcttccatct tcaatgttgt gtctaatttt gaagttaact
     6841 ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt atagttgtat
     6901 tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt taactcgatt
     6961 ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta gttcccgtca
     7021 tctttgaaaa atatagttct ttcctgtacg taaccttcgg gcatggcact cttgaaaaag
     7081 tcatgctgtt tcatgtgatc tgggtatctc gcaaagcatt gaacaccata accgaaagta
     7141 gtgacaagtg ttggccacgg aacaggtagt tttccagtag tgcaaataaa tttaagggta
     7201 agttttccgt atgttgcatc accttcaccc tctccactga cagaaaattt gtgcccatta
     7261 acatcaccat ctaattcaac aagaattggg acaactccag tgaaaagttc ttctccttta
     7321 cgcatattat acctccttaa tttagtgtca tctacaactg gcatgcggtc agtgcgtcct
     7381 gctgatgtgc tcagtatctc tatcactgat agggatgtca atctctatca ctgataggga
     7441 aacgtttcgc agaagcttcc gcaaggtacc actttgccgc ggtcactgcc cgctttccag
     7501 tcgggaaacc tgtcgtgcca gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7561 ttgcgtattg ggagccaggg tggtttttct tttcaccagt gagacgggca acagttgatt
     7621 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     7681 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     7741 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     7801 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     7861 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     7921 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     7981 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     8041 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     8101 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     8161 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgctgcgc
     8221 gagaagattg tgaaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     8281 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     8341 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     8401 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     8461 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     8521 gacaccggca tactctgcga catcgtacag cgttactggt ttcattgtac accctctcct
     8581 tatattggga cctatggatc cggtcagtgc gtcctgctga tgtgctcagt atctctatca
     8641 ctgataggga tgtcaatctc tatcactgat agggacggcc gcccgttcca tggctgaaat
     8701 gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag cggataacaa
     8761 tttcacacgt cgaccgcctt cggcgaagct agggacgaga gctagcatgt ctcgtttaga
     8821 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
     8881 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
     8941 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
     9001 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
     9061 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
     9121 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
     9181 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
     9241 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
     9301 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
     9361 cttcttattc ggccttgaat tgatcatctg cggattagaa aaacaactta aatgtgaaag
     9421 tgggtcttga gagctcggac tgcttaagtc gctccatatg ctcgttcccg ggctgaaatg
     9481 agctgttgac aattaatcat ccggctcgta taatgtgtgg aattgtgagc ggataacaat
     9541 ttcacaccaa ttgataatat acaagattaa ggaggtaaag aatggtgagc aagggcgagg
     9601 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggttcacatg gagggctccg
     9661 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     9721 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     9781 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     9841 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     9901 gcggcgtggt gaccgtgacc caggactcct ccctgcaaga cggcgagttc atctacaagg
     9961 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagactatgg
    10021 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgcgctgaag ggcgagatca
    10081 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
    10141 aggccaagaa gcccgtgcaa ctgcccggcg cgtacaacgt caacatcaag ttggacatca
    10201 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
    10261 ccaccggcgg catggacgag ctgtataagt aagatatcta tcgccctagg gaccgtctcg
    10321 agagaatcaa tattaatcca acgcgtggca tcaaataaaa cgaaaggctc agtcgaaaga
    10381 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
    10441 gccgccctag acttaggcgt tcggctgcgg cgagcggtat cagctcactc aaag
//
