LOCUS       40924_5539       10512 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAC-SC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Creating Single-Copy Genetic Circuits
  JOURNAL   Mol. Cell 63 (2), 329-336 (2016)
  PUBMED    27425413
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St,
            MIT Building E25-Rm302, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 10512)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10512)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell";
            date: "2016"; volume: "63"; issue: "2"; pages: "329-336"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St, MIT 
            Building E25-Rm302, Cambridge, MA 02142, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(105..1073)
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(1076..2248)
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(2574..2824)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(2830..3582)
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     rep_origin      complement(3673..3892)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      complement(3968..4582)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        5440..5542
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5543..6199
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      complement(6455..6549)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6612..7325)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(7377..7450)
                     /label=PLtetO
                     /note="PLtetO"
                     /regulatory_class="promoter"
     promoter        complement(7377..7450)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     misc_feature    7396
                     /label=transcription start
                     /note="transcription start"
     protein_bind    7407..7425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7432..7450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(7496..8575)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(8612..8685)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     promoter        8717..8746
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     prim_transcript 8752
                     /label=transcription start
                     /note="transcription start"
     protein_bind    8754..8770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8817..9437
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      9483..9557
                     /label=Ptrc-2
                     /note="Ptrc-2"
                     /regulatory_class="promoter"
     promoter        9496..9525
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    9533..9549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     prim_transcript 9533
                     /label=transcription start
                     /note="transcription start"
     CDS             9600..10307
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      10370..10456
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagtggggcc cagagagaat attcaggcca gttatgcttt ctggcctgta acaaaggaca
       61 ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc tggcttttca
      121 agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg ggacctgtcc
      181 aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc aaactgatgt
      241 cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt taaaagagtg
      301 ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag gttagatgcc
      361 tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc acctgaccgg
      421 gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa tttggcggtg
      481 ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag cgcagaaata
      541 tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc ataagcactt
      601 gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc atccagctcg
      661 ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg gcgactccca
      721 tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg ttgaccagtc
      781 agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc ctggtcacgt
      841 tcattacctg accatacccg agaggtcttc tcaacactat caccccggag cacttcaaga
      901 gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg agccattact
      961 cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa cgaagtatct
     1021 tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt cattatctaa
     1081 tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt tcccaaatag
     1141 aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt tcaaaaacag
     1201 ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca ttttttagaa
     1261 ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga gagccattac
     1321 tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta agatcaacgt
     1381 tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag tcaaacaact
     1441 cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc gtgccgatac
     1501 ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca gtttcaatgg
     1561 ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat ttgcccatta
     1621 actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc cccggccagc
     1681 aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa ggcaggagag
     1741 tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag gctgttccct
     1801 gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga tcctgagcaa
     1861 gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc ccgatcaccg
     1921 gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc atatgattaa
     1981 tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc atatccgggt
     2041 gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc ccaactaaat
     2101 ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg ctgtcatcat
     2161 taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg cactggttaa
     2221 gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt tttttattaa
     2281 atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa accgcaaagt
     2341 tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc tcagtcactt
     2401 attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac tggcgaggaa
     2461 gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga aatatccacc
     2521 gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt aataaactgt
     2581 gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca catgacgaag
     2641 ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc ttaaaaatta
     2701 cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa ctgtgacaaa
     2761 ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg acagataacc
     2821 ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga tatgagtcgt
     2881 ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc tgttgatctc
     2941 attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt aactttgagg
     3001 cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg agccatccgg
     3061 cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg tgatttcttt
     3121 tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga agggaatgag
     3181 atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac gtttgataaa
     3241 ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc gataaaaaac
     3301 cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg cttccgcaga
     3361 ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac cgtcatgttc
     3421 ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca tacgtttttg
     3481 atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga ttcgcgggct
     3541 atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc catgtgaagt
     3601 gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag ggataacttt
     3661 gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa tttgtcacag
     3721 acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc acaacacctt
     3781 ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga tctaaaaact
     3841 ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca agggaagttt
     3901 tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct tcctcgctca
     3961 ctcgaccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca
     4021 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa
     4081 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc
     4141 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc
     4201 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg
     4261 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc
     4321 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc
     4381 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca
     4441 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cggccgcggt
     4501 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
     4561 gcccagcggc gagggcaacc agccgagggc ttcgccctgt cgctcgactg cggcgagcac
     4621 tactggctgt aaaaggacag accacatcat ggttctgtgt tcattaggtt gttctgtcca
     4681 ttgctgacat aatccgctcc acttcaacgt aacaccgcac gaagatttct attgttcctg
     4741 aaggcatatt caaatcgttt tcgttaccgc ttgcaggcat catgacagaa cactacttcc
     4801 tataaacgct acacaggctc ctgagattaa taatgcggat ctctacgata atgggagatt
     4861 ttcccgactg tttcgttcgc ttctcagtgg ataacagcca gcttctctgt ttaacagaca
     4921 aaaacagcat atccactcag ttccacattt ccatataaag gccaaggcat ttattctcag
     4981 gataattgtt tcagcatcgc aaccgcatca gactccggca tcgcaaactg cacccggtgc
     5041 cgggcagcca catccagcgc aaaaaccttc gtgtagactt ccgttgaact gatggactta
     5101 tgtcccatca ggctttgcag aactttcagc ggtataccgg catacagcat gtgcatcgca
     5161 taggaatggc ggaacgtatg tggtgtgacc ggaacagaga acgtcacacc gtcagcagca
     5221 gcggcggcaa ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc
     5281 cgcgctttct ctgtccttcc tgtgcgacgg ttacgccgct ccatgagctt atcgcgaata
     5341 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
     5401 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
     5461 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     5521 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     5581 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     5641 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     5701 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     5761 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     5821 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     5881 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     5941 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     6001 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     6061 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     6121 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     6181 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     6241 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg
     6301 tcaaacatga gaattggtcg agacggtccc tagggcgata gatatcaacc ctggcgccgg
     6361 gggacaattg tgtagtcccg ggaacgagca tatggagcga cttaagcagt ccgagctcca
     6421 gacggctagc gtaatggtcg acgagtatgc ttggattctc accaataaaa aacgcccggc
     6481 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     6541 acaggagtcc aagcgacgtc tgtgcaagta ctactgttct gcagtcactt gaattcgata
     6601 cccagctgtt atttgtatag ttcatccatg ccatgtgtaa tcccagcagc ggttacaaac
     6661 tcaagaagga ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc agattgtgtg
     6721 gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caatcggagt attttgttga
     6781 taatggtctg ctagttgaac gcttccatct tcaatgttgt gtctaatttt gaagttaact
     6841 ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt atagttgtat
     6901 tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt taactcgatt
     6961 ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta gttcccgtca
     7021 tctttgaaaa atatagttct ttcctgtacg taaccttcgg gcatggcact cttgaaaaag
     7081 tcatgctgtt tcatgtgatc tgggtatctc gcaaagcatt gaacaccata accgaaagta
     7141 gtgacaagtg ttggccacgg aacaggtagt tttccagtag tgcaaataaa tttaagggta
     7201 agttttccgt atgttgcatc accttcaccc tctccactga cagaaaattt gtgcccatta
     7261 acatcaccat ctaattcaac aagaattggg acaactccag tgaaaagttc ttctccttta
     7321 cgcatgtgca ctacctcctt ggttgtctat gctatgctga tctacaactg gcatgcggtc
     7381 agtgcgtcct gctgatgtgc tcagtatctc tatcactgat agggatgtca atctctatca
     7441 ctgataggga aacgtttcgc agaagcttcc gcaaggtacc actttgccgc ggtcactgcc
     7501 cgctttccag tcgggaaacc tgtcgtgcca gttgcattaa tgaatcggcc aacgcgcggg
     7561 gagaggcggt ttgcgtattg ggagccaggg tggtttttct tttcaccagt gagacgggca
     7621 acagttgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     7681 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     7741 tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact
     7801 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     7861 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     7921 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     7981 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     8041 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     8101 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     8161 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     8221 cgcgctgcgc gagaagattg tgaaccgccg ctttacaggc ttcgacgccg cttcgttcta
     8281 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     8341 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     8401 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     8461 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     8521 tctgataaga gacaccggca tactctgcga catcgtacag cgttactggt ttcattgtac
     8581 accctctcct tatattggga cctatggatc cggtcagtgc gtcctgctga tgtgctcagt
     8641 atctctatca ctgataggga tgtcaatctc tatcactgat agggacggcc gcccgttcca
     8701 tggctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag
     8761 cggataacaa tttcacacgt cgaccgcctt cggcgaagct agggacgaga gctagcatgt
     8821 ctcgtttaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
     8881 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
     8941 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
     9001 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
     9061 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
     9121 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
     9181 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
     9241 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
     9301 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
     9361 cagagccagc cttcttattc ggccttgaat tgatcatctg cggattagaa aaacaactta
     9421 aatgtgaaag tgggtcttga gagctcggac tgcttaagtc gctccatatg ctcgttcccg
     9481 ggctgaaatg agctgttgac aattaatcat ccggctcgta taatgtgtgg aattgtgagc
     9541 ggataacaat ttcacaccaa ttgagtatta actatcgttc aactgatagg gaggcgccga
     9601 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     9661 ttcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     9721 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     9781 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     9841 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     9901 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaagacg
     9961 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
    10021 tgcagaagaa gactatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
    10081 cgctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
    10141 aggtcaagac cacctacaag gccaagaagc ccgtgcaact gcccggcgcg tacaacgtca
    10201 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
    10261 gcgccgaggg ccgccactcc accggcggca tggacgagct gtataagtaa gatatctatc
    10321 gccctaggga ccgtctcgag agaatcaata ttaatccaac gcgtggcatc aaataaaacg
    10381 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
    10441 cctgagtagg acaaatccgc cgccctagac ttaggcgttc ggctgcggcg agcggtatca
    10501 gctcactcaa ag
//
