LOCUS       V009335                10980 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009335
VERSION     V009335
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Creating Single-Copy Genetic Circuits
  JOURNAL   Mol. Cell 63 (2), 329-336 (2016)
   PUBMED   27425413
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St,
            MIT Building E25-Rm302, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 10980)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell";
            date: "2016"; volume: "63"; issue: "2"; pages: "329-336"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St, MIT
            Building E25-Rm302, Cambridge, MA 02142, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(105..1073)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(1076..2248)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(2574..2824)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(2830..3582)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(3673..3892)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     rep_origin      complement(3968..4582)
                     /direction=LEFT
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     misc_feature    5425..5472
                     /label="FRT site"
                     /note="FRT site"
     protein_bind    complement(5425..5472)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5496..5598
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5599..6255
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(6384..6417)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    complement(6437..6663)
                     /label="HK022 attP"
                     /note="attachment site of phage HK022"
     terminator      complement(6717..6811)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(6862..7692)
                     /gene="ecoRIR"
                     /label="Type II restriction enzyme EcoRI"
                     /note="Type II restriction enzyme EcoRI from Escherichia
                     coli. Accession#: P00642"
     regulatory      complement(7720..7793)
                     /label="PLtetO"
                     /note="PLtetO"
                     /regulatory_class="promoter"
     promoter        complement(7720..7793)
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     prim_transcript 7739
                     /label="transcription start"
                     /note="transcription start"
     protein_bind    7775..7793
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    7836..7919
                     /label="mfLon-specific tag pdt2"
                     /note="mfLon-specific tag pdt2"
     CDS             complement(7920..8999)
                     /label="lacI"
                     /note="lac repressor"
     RBS             9005..9013
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(9045..9118)
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    9137..9156
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     protein_bind    9173..9192
                     /label="lac operator (symmetric)"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     promoter        9193..9222
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    complement(9229..9248)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     regulatory      9261..9292
                     /label="T4"
                     /note="T4"
                     /regulatory_class="ribosome_binding_site"
     RBS             9279..9287
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9293..9913
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        9972..10001
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    10009..10025
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      10041..10067
                     /label="M2"
                     /note="M2"
                     /regulatory_class="ribosome_binding_site"
     RBS             10054..10062
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             10068..10775
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      10838..10924
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagtggggcc cagagagaat attcaggcca gttatgcttt ctggcctgta acaaaggaca
       61 ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc tggcttttca
      121 agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg ggacctgtcc
      181 aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc aaactgatgt
      241 cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt taaaagagtg
      301 ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag gttagatgcc
      361 tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc acctgaccgg
      421 gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa tttggcggtg
      481 ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag cgcagaaata
      541 tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc ataagcactt
      601 gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc atccagctcg
      661 ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg gcgactccca
      721 tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg ttgaccagtc
      781 agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc ctggtcacgt
      841 tcattacctg accatacccg agaggtcttc tcaacactat caccccggag cacttcaaga
      901 gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg agccattact
      961 cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa cgaagtatct
     1021 tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt cattatctaa
     1081 tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt tcccaaatag
     1141 aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt tcaaaaacag
     1201 ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca ttttttagaa
     1261 ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga gagccattac
     1321 tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta agatcaacgt
     1381 tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag tcaaacaact
     1441 cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc gtgccgatac
     1501 ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca gtttcaatgg
     1561 ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat ttgcccatta
     1621 actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc cccggccagc
     1681 aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa ggcaggagag
     1741 tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag gctgttccct
     1801 gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga tcctgagcaa
     1861 gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc ccgatcaccg
     1921 gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc atatgattaa
     1981 tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc atatccgggt
     2041 gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc ccaactaaat
     2101 ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg ctgtcatcat
     2161 taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg cactggttaa
     2221 gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt tttttattaa
     2281 atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa accgcaaagt
     2341 tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc tcagtcactt
     2401 attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac tggcgaggaa
     2461 gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga aatatccacc
     2521 gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt aataaactgt
     2581 gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca catgacgaag
     2641 ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc ttaaaaatta
     2701 cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa ctgtgacaaa
     2761 ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg acagataacc
     2821 ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga tatgagtcgt
     2881 ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc tgttgatctc
     2941 attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt aactttgagg
     3001 cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg agccatccgg
     3061 cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg tgatttcttt
     3121 tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga agggaatgag
     3181 atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac gtttgataaa
     3241 ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc gataaaaaac
     3301 cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg cttccgcaga
     3361 ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac cgtcatgttc
     3421 ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca tacgtttttg
     3481 atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga ttcgcgggct
     3541 atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc catgtgaagt
     3601 gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag ggataacttt
     3661 gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa tttgtcacag
     3721 acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc acaacacctt
     3781 ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga tctaaaaact
     3841 ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca agggaagttt
     3901 tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct tcctcgctca
     3961 ctcgaccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca
     4021 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa
     4081 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc
     4141 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc
     4201 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg
     4261 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc
     4321 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc
     4381 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca
     4441 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cggccgcggt
     4501 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
     4561 gcccagcggc gagggcaacc agccgagggc ttcgccctgt cgctcgactg cggcgagcac
     4621 tactggctgt aaaaggacag accacatcat ggttctgtgt tcattaggtt gttctgtcca
     4681 ttgctgacat aatccgctcc acttcaacgt aacaccgcac gaagatttct attgttcctg
     4741 aaggcatatt caaatcgttt tcgttaccgc ttgcaggcat catgacagaa cactacttcc
     4801 tataaacgct acacaggctc ctgagattaa taatgcggat ctctacgata atgggagatt
     4861 ttcccgactg tttcgttcgc ttctcagtgg ataacagcca gcttctctgt ttaacagaca
     4921 aaaacagcat atccactcag ttccacattt ccatataaag gccaaggcat ttattctcag
     4981 gataattgtt tcagcatcgc aaccgcatca gactccggca tcgcaaactg cacccggtgc
     5041 cgggcagcca catccagcgc aaaaaccttc gtgtagactt ccgttgaact gatggactta
     5101 tgtcccatca ggctttgcag aactttcagc ggtataccgg catacagcat gtgcatcgca
     5161 taggaatggc ggaacgtatg tggtgtgacc ggaacagaga acgtcacacc gtcagcagca
     5221 gcggcggcaa ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc
     5281 cgcgctttct ctgtccttcc tgtgcgacgg ttacgccgct ccatgagctt atcgcgaata
     5341 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
     5401 cgggaagccc catatggatc ccgggaagtt cctatacttt ctagagaata ggaacttcgg
     5461 aataggaact tcaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
     5521 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     5581 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     5641 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     5701 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     5761 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
     5821 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     5881 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     5941 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     6001 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     6061 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt cacgatgggc
     6121 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     6181 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     6241 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc
     6301 tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgatgat aagctgtcac
     6361 ggctgacatg ggaattagcc atggaagttc ctatactttc tagagaatag gaacttcggg
     6421 acaaaattga aatcgacaaa tgattttatt ttgactaata atgacctact tacattaatt
     6481 tactgataat taaagagatt ttaaatatac aacttattca cctaaaggat gacaaaataa
     6541 cattaatcac ttaaaaatca tcgcattaca ctaatctgtg gttaaatgat agactacata
     6601 atgcgacaaa acgcaacata tccagtcact atgaatcaac tacttagata gtattagtga
     6661 cctgagacag agcattagct agaaacatga gaattggtct cgacgagtat gcttggattc
     6721 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     6781 gaggtcatta ctggatctat caacaggagt ccaagcgacg tctgtgcaag tactactgtt
     6841 ctgcagtcac ttgaattctc acttagatgt aagctgttca aacaagtcac gccccaacac
     6901 tctgagcgaa gtcgttgata tatcaaacat tatttcaaac atgattttcg aatcccactc
     6961 cctcccatct ccttgagtat atatagatgc tgcttgtagc ataatgcttt tgtctttatg
     7021 atttacaaat ttgttaatac atagattact atttataggc attccataat tagctgcagt
     7081 tagtcgatct aacctattta atataccaga attatactca agattaacaa cccttccatc
     7141 tggtcttgtt attgagatat tttctgttaa aaagttagac ccctctaaga aaaggacgta
     7201 aggaaagtgg ctctcagaga gcataaaatt cgctatctct gatatattct tatgagatct
     7261 ttcgatagca ttaccagcag ccattaaatc ttgatctcct cttttcccaa ctaacaaacc
     7321 attccttata tttataatat ctttaccttg gtgtttggct tcagcaacaa gtacaactct
     7381 ccattcacca taatcatctt tgacctctac aattccacca tcaggtttga tgctggaatt
     7441 tgaaacaaat aaagtaccgc caagatcagg gtcaattttt tttaaagctt catttatttc
     7501 tgttttcttt atactatctc tatatcgaaa tgataattga gggtattcgt tgctaagagc
     7561 tttctttact aattttgaaa cctcaccaac agcgagatca tgagcttttg cataatcccc
     7621 aaaaatccca attacacctt gagataactt atgttgttca gttagcctat ttgactgttt
     7681 tttattagac atgaatccat gtttccactg ttagcatgcg gtcagtgcgt cctgctgatg
     7741 tgctcagtat atctatcact gatagggatg tcaatctcta tcactgatag ggaaacgttt
     7801 cgcagaagct tccgcaaggt accactttgc cgcggttatt gagacaggta gttcgcctgg
     7861 cccgcgttca gcataaaggt cggcacttcg ttggtgtttt cttcgttttt gttcgccgcc
     7921 tgcccgcttt ccagtcggga aacctgtcgt gccagttgca ttaatgaatc ggccaacgcg
     7981 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     8041 ggcaacagtt gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     8101 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     8161 gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg
     8221 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     8281 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     8341 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     8401 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     8461 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     8521 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     8581 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     8641 ctgacgcgct gcgcgagaag attgtgaacc gccgctttac aggcttcgac gccgcttcgt
     8701 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     8761 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     8821 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     8881 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     8941 acggtctgat aagagacacc ggcatactct gcgacatcgt acagcgttac tggtttcata
     9001 ttgtacctcc ttaattcttg attttctatc ctcgtcatgt gagcggtcag tgcgtcctgc
     9061 tgatgtgctc agtatatcta tcactgatag ggatgtcaat ctctatcact gatagggacg
     9121 gccgcccgtt ccatggaatt gtgagcgctc acaattacta gcggccgcat ggaattgtga
     9181 gcgctcacaa ttttgacaat taatcatccg gctcgtataa tgtgtgggaa ttgtgagcgc
     9241 tcacaatttc acacgtcgac gcaccataaa gcggaaagta aggaggttag atatgtctcg
     9301 tttagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga
     9361 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg
     9421 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca
     9481 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc
     9541 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac
     9601 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca
     9661 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg
     9721 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac
     9781 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga
     9841 gccagccttc ttattcggcc ttgaattgat catctgcgga ttagaaaaac aacttaaatg
     9901 tgaaagtggg tcttgagagc tcggactgct taagtcgctc catatgctcg ttcccgggct
     9961 gaaatgagct gttgacaatt aatcatccgg ctcgtataat gtgtggaatt gtgagcggat
    10021 aacaatttca caccaattga taatatacaa gattaaggag gtaaagaatg gtgagcaagg
    10081 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtt cacatggagg
    10141 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
    10201 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
    10261 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
    10321 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
    10381 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaagacggc gagttcatct
    10441 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
    10501 ctatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcg ctgaagggcg
    10561 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
    10621 cctacaaggc caagaagccc gtgcaactgc ccggcgcgta caacgtcaac atcaagttgg
    10681 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
    10741 gccactccac cggcggcatg gacgagctgt ataagtaaga tatctatcgc cctagggacc
    10801 gtctcgagag aatcaatatt aatccaacgc gtggcatcaa ataaaacgaa aggctcagtc
    10861 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
    10921 aaatccgccg ccctagactt aggcgttcgg ctgcggcgag cggtatcagc tcactcaaag
//
