LOCUS       40924_5519       10452 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAC-L6-PT3-T4Ci, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Creating Single-Copy Genetic Circuits
  JOURNAL   Mol. Cell 63 (2), 329-336 (2016)
  PUBMED    27425413
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
            Del Vecchio D, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St,
            MIT Building E25-Rm302, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 10452)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell";
            date: "2016"; volume: "63"; issue: "2"; pages: "329-336"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St, MIT 
            Building E25-Rm302, Cambridge, MA 02142, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(105..1073)
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(1076..2248)
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(2574..2824)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(2830..3582)
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     rep_origin      complement(3673..3892)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      complement(3968..4582)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     misc_feature    5425..5472
                     /label=FRT site
                     /note="FRT site"
     protein_bind    complement(5425..5472)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5496..5598
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5599..6255
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(6384..6417)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    complement(6437..6663)
                     /label=HK022 attP
                     /note="attachment site of phage HK022"
     terminator      complement(6717..6811)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6862..7164)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      complement(7171..7185)
                     /label=RBS
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(7192..7265)
                     /label=PLtetO
                     /note="PLtetO"
                     /regulatory_class="promoter"
     promoter        complement(7192..7265)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     prim_transcript 7211
                     /label=transcription start
                     /note="transcription start"
     protein_bind    7247..7265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    complement(7308..7391)
                     /label=mfLon-specific tag pdt2
                     /note="mfLon-specific tag pdt2"
     CDS             complement(7392..8471)
                     /label=lacI
                     /note="lac repressor"
     RBS             8477..8485
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(8517..8590)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    8609..8628
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8645..8664
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        8665..8694
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    complement(8701..8720)
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     regulatory      8733..8764
                     /label=T4
                     /note="T4"
                     /regulatory_class="ribosome_binding_site"
     RBS             8751..8759
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8765..9385
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      9431..9505
                     /label=Ptrc-2
                     /note="Ptrc-2"
                     /regulatory_class="promoter"
     promoter        9444..9473
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    9481..9497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     prim_transcript 9481
                     /label=transcription start
                     /note="transcription start"
     regulatory      9513..9539
                     /label=M2
                     /note="M2"
                     /regulatory_class="ribosome_binding_site"
     RBS             9526..9534
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9540..10247
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      10310..10396
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagtggggcc cagagagaat attcaggcca gttatgcttt ctggcctgta acaaaggaca
       61 ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc tggcttttca
      121 agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg ggacctgtcc
      181 aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc aaactgatgt
      241 cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt taaaagagtg
      301 ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag gttagatgcc
      361 tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc acctgaccgg
      421 gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa tttggcggtg
      481 ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag cgcagaaata
      541 tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc ataagcactt
      601 gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc atccagctcg
      661 ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg gcgactccca
      721 tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg ttgaccagtc
      781 agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc ctggtcacgt
      841 tcattacctg accatacccg agaggtcttc tcaacactat caccccggag cacttcaaga
      901 gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg agccattact
      961 cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa cgaagtatct
     1021 tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt cattatctaa
     1081 tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt tcccaaatag
     1141 aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt tcaaaaacag
     1201 ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca ttttttagaa
     1261 ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga gagccattac
     1321 tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta agatcaacgt
     1381 tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag tcaaacaact
     1441 cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc gtgccgatac
     1501 ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca gtttcaatgg
     1561 ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat ttgcccatta
     1621 actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc cccggccagc
     1681 aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa ggcaggagag
     1741 tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag gctgttccct
     1801 gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga tcctgagcaa
     1861 gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc ccgatcaccg
     1921 gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc atatgattaa
     1981 tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc atatccgggt
     2041 gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc ccaactaaat
     2101 ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg ctgtcatcat
     2161 taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg cactggttaa
     2221 gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt tttttattaa
     2281 atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa accgcaaagt
     2341 tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc tcagtcactt
     2401 attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac tggcgaggaa
     2461 gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga aatatccacc
     2521 gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt aataaactgt
     2581 gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca catgacgaag
     2641 ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc ttaaaaatta
     2701 cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa ctgtgacaaa
     2761 ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg acagataacc
     2821 ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga tatgagtcgt
     2881 ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc tgttgatctc
     2941 attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt aactttgagg
     3001 cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg agccatccgg
     3061 cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg tgatttcttt
     3121 tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga agggaatgag
     3181 atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac gtttgataaa
     3241 ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc gataaaaaac
     3301 cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg cttccgcaga
     3361 ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac cgtcatgttc
     3421 ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca tacgtttttg
     3481 atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga ttcgcgggct
     3541 atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc catgtgaagt
     3601 gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag ggataacttt
     3661 gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa tttgtcacag
     3721 acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc acaacacctt
     3781 ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga tctaaaaact
     3841 ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca agggaagttt
     3901 tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct tcctcgctca
     3961 ctcgaccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca
     4021 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa
     4081 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc
     4141 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc
     4201 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg
     4261 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc
     4321 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc
     4381 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca
     4441 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cggccgcggt
     4501 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
     4561 gcccagcggc gagggcaacc agccgagggc ttcgccctgt cgctcgactg cggcgagcac
     4621 tactggctgt aaaaggacag accacatcat ggttctgtgt tcattaggtt gttctgtcca
     4681 ttgctgacat aatccgctcc acttcaacgt aacaccgcac gaagatttct attgttcctg
     4741 aaggcatatt caaatcgttt tcgttaccgc ttgcaggcat catgacagaa cactacttcc
     4801 tataaacgct acacaggctc ctgagattaa taatgcggat ctctacgata atgggagatt
     4861 ttcccgactg tttcgttcgc ttctcagtgg ataacagcca gcttctctgt ttaacagaca
     4921 aaaacagcat atccactcag ttccacattt ccatataaag gccaaggcat ttattctcag
     4981 gataattgtt tcagcatcgc aaccgcatca gactccggca tcgcaaactg cacccggtgc
     5041 cgggcagcca catccagcgc aaaaaccttc gtgtagactt ccgttgaact gatggactta
     5101 tgtcccatca ggctttgcag aactttcagc ggtataccgg catacagcat gtgcatcgca
     5161 taggaatggc ggaacgtatg tggtgtgacc ggaacagaga acgtcacacc gtcagcagca
     5221 gcggcggcaa ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc
     5281 cgcgctttct ctgtccttcc tgtgcgacgg ttacgccgct ccatgagctt atcgcgaata
     5341 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
     5401 cgggaagccc catatggatc ccgggaagtt cctatacttt ctagagaata ggaacttcgg
     5461 aataggaact tcaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
     5521 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     5581 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     5641 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     5701 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     5761 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
     5821 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     5881 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     5941 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     6001 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     6061 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt cacgatgggc
     6121 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     6181 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     6241 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc
     6301 tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgatgat aagctgtcac
     6361 ggctgacatg ggaattagcc atggaagttc ctatactttc tagagaatag gaacttcggg
     6421 acaaaattga aatcgacaaa tgattttatt ttgactaata atgacctact tacattaatt
     6481 tactgataat taaagagatt ttaaatatac aacttattca cctaaaggat gacaaaataa
     6541 cattaatcac ttaaaaatca tcgcattaca ctaatctgtg gttaaatgat agactacata
     6601 atgcgacaaa acgcaacata tccagtcact atgaatcaac tacttagata gtattagtga
     6661 cctgagacag agcattagct agaaacatga gaattggtct cgacgagtat gcttggattc
     6721 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     6781 gaggtcatta ctggatctat caacaggagt ccaagcgacg tctgtgcaag tactactgtt
     6841 ctgcagtcac ttgaattctt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
     6901 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
     6961 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
     7021 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
     7081 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
     7141 ataggtgtaa accttaaact gcatggtata tttcttatat ttaatgcatg cggtcagtgc
     7201 gtcctgctga tgtgctcagt atatctatca ctgataggga tgtcaatctc tatcactgat
     7261 agggaaacgt ttcgcagaag cttccgcaag gtaccacttt gccgcggtta ttgagacagg
     7321 tagttcgcct ggcccgcgtt cagcataaag gtcggcactt cgttggtgtt ttcttcgttt
     7381 ttgttcgccg cctgcccgct ttccagtcgg gaaacctgtc gtgccagttg cattaatgaa
     7441 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
     7501 accagtgaga cgggcaacag ttgattgccc ttcaccgcct ggccctgaga gagttgcagc
     7561 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
     7621 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca
     7681 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     7741 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     7801 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     7861 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     7921 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     7981 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     8041 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     8101 atgatcagcc cactgacgcg ctgcgcgaga agattgtgaa ccgccgcttt acaggcttcg
     8161 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     8221 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     8281 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     8341 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     8401 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtacagcgtt
     8461 actggtttca tattgtacct ccttaattct tgattttcta tcctcgtcat gtgagcggtc
     8521 agtgcgtcct gctgatgtgc tcagtatatc tatcactgat agggatgtca atctctatca
     8581 ctgataggga cggccgcccg ttccatggaa ttgtgagcgc tcacaattac tagcggccgc
     8641 atggaattgt gagcgctcac aattttgaca attaatcatc cggctcgtat aatgtgtggg
     8701 aattgtgagc gctcacaatt tcacacgtcg acgcaccata aagcggaaag taaggaggtt
     8761 agatatgtct cgtttagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
     8821 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
     8881 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
     8941 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
     9001 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
     9061 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
     9121 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
     9181 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
     9241 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
     9301 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatctgcg gattagaaaa
     9361 acaacttaaa tgtgaaagtg ggtcttgaga gctcggactg cttaagtcgc tccatatgct
     9421 cgttcccggg ctgaaatgag ctgttgacaa ttaatcatcc ggctcgtata atgtgtggaa
     9481 ttgtgagcgg ataacaattt cacaccaatt gataatatac aagattaagg aggtaaagaa
     9541 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     9601 ttcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     9661 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     9721 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     9781 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     9841 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaagacg
     9901 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     9961 tgcagaagaa gactatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
    10021 cgctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
    10081 aggtcaagac cacctacaag gccaagaagc ccgtgcaact gcccggcgcg tacaacgtca
    10141 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
    10201 gcgccgaggg ccgccactcc accggcggca tggacgagct gtataagtaa gatatctatc
    10261 gccctaggga ccgtctcgag agaatcaata ttaatccaac gcgtggcatc aaataaaacg
    10321 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
    10381 cctgagtagg acaaatccgc cgccctagac ttaggcgttc ggctgcggcg agcggtatca
    10441 gctcactcaa ag
//
