LOCUS       V009341                 8821 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009341
VERSION     V009341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Na G, Wolfe A, Ko C, Youn H, Lee YM, Byun SJ, Jeon I, Koo Y.
  TITLE     A Low-Copy-Number Plasmid for Retrieval of Toxic Genes from BACs and
            Generation of Conditional Targeting Constructs
  JOURNAL   Mol. Biotechnol. 54 (2), 504-514 (2013)
   PUBMED   22945876
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Koo Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2010) Biological Sciences, Inje University, 607
            Obangdong, Gimae, Gyongnam 621-749, South Korea
REFERENCE   3  (bases 1 to 8821)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Biotechnol."; date: "2013"; volume: "54"; issue: "2"; pages:
            "504-514"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-NOV-2010) Biological Sciences, Inje University, 607 Obangdong,
            Gimae, Gyongnam 621-749, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..27
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(57..75)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             175..1590
                     /gene="sacB"
                     /label="Levansucrase"
                     /note="Levansucrase from Bacillus amyloliquefaciens.
                     Accession#: P21130"
     promoter        complement(2015..2043)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     protein_bind    2072..2105
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    complement(2375..2848)
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2924..3892)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(3895..5067)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(5393..5643)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(5649..6401)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(6492..6711)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     promoter        7638..7740
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             7741..8397
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(8649..8682)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
ORIGIN
        1 gcggccgcta atacgactca ctatagggag aggatccgcg gaattccgcg gatccttcta
       61 tagtgtcacc taaatgtcga cggccaggcg gccgccaggc ctacccacta gtcaattcgg
      121 gaggatcgaa acggcagatc gcaaaaaaca gtacatacag aaggagacat gaacatgaac
      181 atcaaaaaaa ttgtaaaaca agccacagtt ctgactttta cgactgcact tctggcagga
      241 ggagcgactc aagccttcgc gaaagaaaat aaccaaaaag catacaaaga aacgtacggc
      301 gtctctcata ttacacgcca tgatatgctg cagatcccta aacagcagca aaacgaaaaa
      361 taccaagtgc ctcaattcga tcaatcaacg attaaaaata ttgagtctgc aaaaggactt
      421 gatgtgtggg acagctggcc gctgcaaaac gctgacggaa cagtagctga atacaacggc
      481 tatcacgttg tgtttgctct tgcgggaagc ccgaaagacg ctgatgacac atcaatctac
      541 atgttttatc aaaaggtcgg cgacaactca atcgacagct ggaaaaacgc gggccgtgtc
      601 tttaaagaca gcgataagtt cgacgccaac gatccgatcc tgaaagatca gacgcaagaa
      661 tggtccggtt ctgcaacctt tacatctgac ggaaaaatcc gtttattcta cactgactat
      721 tccggtaaac attacggcaa acaaagcctg acaacagcgc aggtaaatgt gtcaaaatct
      781 gatgacacac tcaaaatcaa cggagtggaa gatcacaaaa cgatttttga cggagacgga
      841 aaaacatatc agaacgttca gcagtttatc gatgaaggca attatacatc cggcgacaac
      901 catacgctga gagaccctca ctacgttgaa gacaaaggcc ataaatacct tgtattcgaa
      961 gccaacacgg gaacagaaaa cggataccaa ggcgaagaat ctttatttaa caaagcgtac
     1021 tacggcggcg gcacgaactt cttccgtaaa gaaagccaga agcttcagca gagcgctaaa
     1081 aaacgcgatg ctgagttagc gaacggcgcc ctcggtatca tagagttaaa taatgattac
     1141 acattgaaaa aagtaatgaa gccgctgatc acttcaaaca cggtaactga tgaaatcgag
     1201 cgcgcgaatg ttttcaaaat gaacggcaaa tggtacttgt tcactgattc acgcggttca
     1261 aaaatgacga tcgatggtat taactcaaac gatatttaca tgcttggtta tgtatcaaac
     1321 tctttaaccg gcccttacaa gccgctgaac aaaacagggc ttgtgctgca aatgggtctt
     1381 gatccaaacg atgtgacatt cacttactct cacttcgcag tgccgcaagc caaaggcaac
     1441 aatgtggtta tcacaagcta catgacaaac agaggcttct tcgaggataa aaaggcaaca
     1501 tttgcgccaa gcttcttaat gaacatcaaa ggcaataaaa catccgttgt caaaaacagc
     1561 atcctggagc aaggacagct gacagtcaac taataacagc aaaaagaaaa tgccgatact
     1621 tcattggcat tttcttttat ttctcaacaa gatggtgaat tgactagtgg gtagatccac
     1681 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
     1741 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
     1801 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
     1861 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     1921 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     1981 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
     2041 caaacatgag aattgatccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     2101 gttattaggt ccctcgacta tagggtcacc gtcgacagcg acacacttgc atcggatgca
     2161 gcccggttaa cgtgccggca cggcctgggt aaccaggtat tttgtccaca taaccgtgcg
     2221 caaaatgttg tggataagca ggacacagca gcaatccaca gcaggcatac aaccgcacac
     2281 cgaggttact ccgttctaca ggttacgacg acatgtcaat acttgccctt gacaggcatt
     2341 gatggaatcg tagtctcacg ctgatagtct gatcgacaat acaagtggga ccgtggtccc
     2401 agaccgataa tcagaccgac aacacgagtg ggatcgtggt cccagactaa taatcagacc
     2461 gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac
     2521 cgtggttcca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
     2581 aatcagaccg acgatacgag tgggaccatg gtcccagact aataatcaga ccgacgatac
     2641 gagtgggacc gtggtcccag tctgattatc agaccgacga tacgagtggg accgtggtcc
     2701 cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac
     2761 cgacgatacg agtgggaccg tggtcccagt ctgattatca gaccgacgat acaagtggaa
     2821 cagtgggccc agagagaata ttcaggccag ttatgctttc tggcctgtaa caaaggacat
     2881 taagtaaaga cagataaacg tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa
     2941 gttccttaag aatggcctca attttctcta tacactcagt tggaacacga gacctgtcca
     3001 ggttaagcac cattttatcg cccttataca atactgtcgc tccaggagca aactgatgtc
     3061 gtgagcttaa actagttctt gatgcagatg acgttttaag cacagaagtt aaaagagtga
     3121 taacttcttc agcttcaaat atcaccccag cttttttctg ctcatgaagg ttagatgcct
     3181 gctgcttaag taattcctct ttatctgtaa aggctttttg aagtgcatca cctgaccggg
     3241 cagatagttc accggggtga gaaaaaagag caacaactga tttaggcaat ttggcggtgt
     3301 tgatacagcg ggtaataatc ttacgtgaaa tattttccgc atcagccagc gcagaaatat
     3361 ttccagcaaa ttcattctgc aatcggcttg cataacgctg accacgttca taagcacttg
     3421 ttgggcgata atcgttaccc aatctggata atgcagccat ctgctcatca tccagctcgc
     3481 caaccagaac acgataatca ctttcggtaa gtgcagcagc tttacgacgg cgactcccat
     3541 cggcaatttc tatgacacca gatactcttc gaccgaacgc cggtgtctgt tgaccagtca
     3601 gtagaaaaga agggatgaga tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt
     3661 cattacctga ccatacccga gaggtcttct caacactatc accccggagc acttcaagag
     3721 taaacttcac atcccgacca catacaggca aagtaatggc attaccgcga gccattactc
     3781 ctacgcgcgc aattaacgaa tccaccatcg gggcagctgg tgtcgataac gaagtatctt
     3841 caaccggttg agtattgagc gtatgttttg gaataacagg cgcacgcttc attatctaat
     3901 ctcccagcgt ggtttaatca gacgatcgaa aatttcattg cagacaggtt cccaaataga
     3961 aagagcattt ctccaggcac cagttgaaga gcgttgatca atggcctgtt caaaaacagt
     4021 tctcatccgg atctgacctt taccaacttc atccgtttca cgtacaacat tttttagaac
     4081 catgcttccc caggcatccc gaatttgctc ctccatccac ggggactgag agccattact
     4141 attgctgtat ttggtaagca aaatacgtac atcaggctcg aaccctttaa gatcaacgtt
     4201 cttgagcaga tcacgaagca tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc
     4261 agcaggcgtg ggaacaatca gcacatcagc agcacatacg acattaatcg tgccgatacc
     4321 caggttaggc gcgctgtcaa taactatgac atcatagtca tgagcaacag tttcaatggc
     4381 cagtcggagc atcaggtgtg gatcggtggg cagtttacct tcatcaaatt tgcccattaa
     4441 ctcagtttca atacggtgca gagccagaca ggaaggaata atgtcaagcc ccggccagca
     4501 agtgggcttt attgcataag tgacatcgtc cttttcccca agatagaaag gcaggagagt
     4561 gtcttctgca tgaatatgaa gatctggtac ccatccgtga tacattgagg ctgttccctg
     4621 ggggtcgtta ccttccacga gcaaaacacg tagccccttc agagccagat cctgagcaag
     4681 atgaacagaa actgaggttt tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg
     4741 tggaaatacg tcttcagcac gtcgcaatcg cgtaccaaac acatcacgca tatgattaat
     4801 ttgttcaatt gtataaccaa cacgttgctc aacccgtcct cgaatttcca tatccgggtg
     4861 cggtagtcgc cctgctttct cggcatctct gatagcctga gaagaaaccc caactaaatc
     4921 cgctgcttca cctattctcc agcgccgggt tattttcctc gcttccgggc tgtcatcatt
     4981 aaactgtgca atggcgatag ccttcgtcat ttcatgacca gcgtttatgc actggttaag
     5041 tgtttccatg agtttcattc tgaacatcct ttaatcattg ctttgcgttt ttttattaaa
     5101 tcttgcaatt tactgcaaag caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt
     5161 gtttaaaata agagcaacac tacaaaagga gataagaaga gcacatacct cagtcactta
     5221 ttatcactag cgctcgccgc agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag
     5281 caaagaagaa ctgttctgtc agatagctct tacgctcagc gcaagaagaa atatccaccg
     5341 tgggaaaaac tccaggtaga ggtacacacg cggatagcca attcagagta ataaactgtg
     5401 ataatcaacc ctcatcaatg atgacgaact aacccccgat atcaggtcac atgacgaagg
     5461 gaaagagaag gaaatcaact gtgacaaact gccctcaaat ttggcttcct taaaaattac
     5521 agttcaaaaa gtatgagaaa atccatgcag gctgaaggaa acagcaaaac tgtgacaaat
     5581 taccctcagt aggtcagaac aaatgtgacg aaccaccctc aaatctgtga cagataaccc
     5641 tcagactatc ctgtcgtcat ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc
     5701 tggcggcctt tctttttctc aatgtatgag aggcgcattg gagttctgct gttgatctca
     5761 ttaacacaga cctgcaggaa gcggcggcgg aagtcaggca tacgctggta actttgaggc
     5821 agctggtaac gctctatgat ccagtcgatt ttcagagaga cgatgcctga gccatccggc
     5881 ttacgatact gacacaggga ttcgtataaa cgcatggcat acggattggt gatttctttt
     5941 gtttcactaa gccgaaactg cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga
     6001 tatgggttga tatgtacact gtaaagccct ctggatggac tgtgcgcacg tttgataaac
     6061 caaggaaaag attcatagcc tttttcatcg ccggcatcct cttcagggcg ataaaaaacc
     6121 acttccttcc ccgcgaaact cttcaatgcc tgccgtatat ccttactggc ttccgcagag
     6181 gtcaatccga atatttcagc atatttagca acatggatct cgcagatacc gtcatgttcc
     6241 tgtagggtgc catcagattt tctgatctgg tcaacgaaca gatacagcat acgtttttga
     6301 tcccgggaga gactatatgc cgcctcagtg aggtcgtttg actggacgat tcgcgggcta
     6361 tttttacgtt tcttgtgatt gataaccgct gtttccgcca tgacagatcc atgtgaagtg
     6421 tgacaagttt ttagattgtc acactaaata aaaaagagtc aataagcagg gataactttg
     6481 tgaaaaaaca gcttcttctg agggcaattt gtcacagggt taagggcaat ttgtcacaga
     6541 caggactgtc atttgagggt gatttgtcac actgaaaggg caatttgtca caacaccttc
     6601 tctagaacca gcatggataa aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta
     6661 taaaaaaaat aattataaaa atatccccgt ggataagtgg ataaccccaa gggaagtttt
     6721 ttcaggcatc gtgtgtaagc agaatatata agtgctgttc cctggtgctt cctcgctcac
     6781 tcgagggctt cgccctgtcg ctcaactgcg gcgagcacta ctggctgtaa aaggacagac
     6841 cacatcatgg ttctgtgttc attaggttgt tctgtccatt gctgacataa tccgctccac
     6901 ttcaacgtaa caccgcacga agatttctat tgttcctgaa ggcatattca aatcgttttc
     6961 gttaccgctt gcaggcatca tgacagaaca ctacttccta taaacgctac acaggctcct
     7021 gagattaata atgcggatct ctacgataat gggagatttt cccgactgtt tcgttcgctt
     7081 ctcagtggat aacagccagc ttctctgttt aacagacaaa aacagcatat ccactcagtt
     7141 ccacatttcc atataaaggc caaggcattt attctcagga taattgtttc agcatcgcaa
     7201 ccgcatcaga ctccggcatc gcaaactgca cccggtgccg ggcagccaca tccagcgcaa
     7261 aaaccttcgt gtagacttcc gttgaactga tggacttatg tcccatcagg ctttgcagaa
     7321 ctttcagcgg tataccggca tacagcatgt gcatcgcata ggaatggcgg aacgtatgtg
     7381 gtgtgaccgg aacagagaac gtcacaccgt cagcagcagc ggcggcaacc gcctccccaa
     7441 tccaggtcct gaccgttctg tccgtcactt cccagatccg cgctttctct gtccttcctg
     7501 tgcgacggtt acgccgctcc atgagcttat cgcgaataaa tacctgtgac ggaagatcac
     7561 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
     7621 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
     7681 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     7741 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     7801 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     7861 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     7921 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt
     7981 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     8041 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     8101 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     8161 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     8221 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     8281 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     8341 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     8401 tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat
     8461 aataagcgga tgaatggcag aaattcgatg ataagctgtc aaacatgaga attggtcgac
     8521 ggcgcgccaa agcttgcatg cctgcagccg cgtaacctgg caaaatcggt tacggttgag
     8581 taataaatgg atgccctgcg taagcggggc acatttcatt acctctttct ccgcacccga
     8641 catagataat aacttcgtat agtatacatt atacgaagtt atctagtaga cttaattaag
     8701 gatcgatccg gcgcgccaat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     8761 aaggctctca agggcatcgg tcgagcttga cattgtagga ctatattgct ctaataaatt
     8821 t
//
